We narrowed to 4,170 results for: spl

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pB-6HIS-TEV-mEGFP,IRES-NGFR

Plasmid

#164521

Purpose

All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal His-TEV-GFP tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

Depositor

Mitchell Guttman

Publication

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Type

Empty backbone

Expression

Mammalian

Available since

Feb. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe-CD44v4

Plasmid

#107969

Purpose

expression of CD44 variant

Depositor

Georg Weber

Insert

mCD44v4 (Cd44 Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

splice variant; see Depositor Comments below

Available since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/mCherry-DMDEx23

Plasmid

#211367

Purpose

Expresses a DMD exon 23 skipping reporter in mammalian cells (mCherry-DMDEx23)

Depositor

Ulrich Lächelt

Publication

Lessl et al Bioconjug Chem. 2023 Dec 20;34(12):2263-

Insert

mCherry-DMDEx23

Tags

eGFP

Expression

Mammalian

Mutation

mCherry interrupted by mdx dystrophin exon 23 bet…

Promoter

CMV

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIP TRE-BI GFP10-RHOB/RBD-GFP11

Plasmid

#182231

Purpose

Inducible coexpression of GFP10-RHOB and RBD-GFP11 domain

Depositor

Stéphanie Cabantous

Publication

Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

Inserts

ras homolog family member B (RHOB Human)
rhotekin RHO binding domain (RBD)

Use

Lentiviral

Tags

GFP10 (split-GFP tripartite) and GFP11 (M4)

Promoter

TRE bidirectional

Available since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pSLED.ENS

Plasmid

#193016

Purpose

Excitatory neuron-specific reporter (hSyn promoter, Synrg exon, bichromatic reporter)

Depositor

Jonathan Ling

Publication

Ling et al Nat Commun. 2022 Oct 1;13(1):5773. doi:

Insert

Bichromatic reporter (EGFP and dsRed-Express2)

Use

AAV

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pETCON-DnvLB16

Plasmid

#45124

Depositor

David Baker

Publication

Procko et al J Mol Biol. 2013 Sep 23;425(18):3563-75.

Insert

DnvLB16

Tags

Aga2p for yeast surface display and c-myc epitope…

Expression

Yeast

Promoter

GAL

Available since

July 5, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-DN-RFX

Plasmid

#52296

Purpose

Dominant-negatively inhibits RFX (regulatory factor X) transcription factor. Made of mouse RFX1 DNA binding domain (400-527 aa) and C-terminal myc tag.

Depositor

Christopher A Walsh

Publication

Bae et al Science. 2014 Feb 14;343(6172):764-8. do

Insert

Dominant negative RFX

Tags

Myc

Expression

Mammalian

Promoter

CAG promoter

Available since

April 16, 2014

Availability

Academic Institutions and Nonprofits only

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pACUH-sfcherry2(1-10)

Plasmid

#172061

Purpose

UAS vector to express sfcherry2(1-10) in Drosophila

Depositor

Daichi Kamiyama

Publication

Kamiyama et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

Insert

sfcherry2(1-10)

Expression

Insect

Available since

Oct. 20, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pSiMPla_N + pSiMPla_C

Plasmid

#134314

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Publication

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

ampR_gp41-1 N-intein + ampR_gp41-1 C-intein

Expression

Bacterial

Available since

March 2, 2020

Availability

Academic Institutions and Nonprofits only

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pET28b-AF0623

Plasmid

#30164

Depositor

SeWon Suh

Publication

Kim do et al Acta Crystallogr Sect F Struct Biol Crys

Insert

AF0623 (lig Archaeoglobus fulgidus)

Tags

His

Expression

Bacterial

Available since

April 19, 2012

Availability

Academic Institutions and Nonprofits only
pCAGGS-3xFLAG-Rbfox3

Plasmid

#213727

Purpose

Mammalian cell expression vector for overexpression of Rbfox3 in motor neurons.

Depositor

Chaolin Zhang

Publication

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

Rbfox3 (Rbfox3 Mouse)

Expression

Mammalian

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3xFLAG-Rbfox2

Plasmid

#213726

Purpose

Mammalian cell expression vector for overexpression of Rbfox2 in motor neurons.

Depositor

Chaolin Zhang

Publication

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

Rbfox2 (Rbfox2 Mouse)

Expression

Mammalian

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3xFLAG-Rbfox1

Plasmid

#213725

Purpose

Mammalian cell expression vector for overexpression of Rbfox1 in motor neurons.

Depositor

Chaolin Zhang

Publication

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

Rbfox1 (Rbfox1 Mouse)

Expression

Mammalian

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pM355

Plasmid

#173175

Purpose

(Empty Backbone) Expresses gRNA-12xMBS from hU6 promoter

Depositor

Hajin Kim

Publication

Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do

Insert

gRNA-12xMBS backbone

Use

CRISPR

Expression

Mammalian

Promoter

hU6

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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UBQ10:sXVE:GFP1–10

Plasmid

#125663

Purpose

Binary vector for the inducible expression of GFP1–10 fragment that can be used to detect a protein tagged with GFP11

Depositor

Tzu-Yin Liu

Publication

Liu et al Methods Mol Biol. 2021;2200:323-336. doi

Insert

GFP1–10

Use

Synthetic Biology

Expression

Plant

Promoter

UBQ10:sXVE:

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pSiMPlh_N + pSiMPlh_C

Plasmid

#134316

Purpose

Maintenance of 2 plasmids with a single antibiotic in E. coli

Depositor

Barbara Di Ventura

Publication

Palanisamy et al Nat Commun. 2019 Oct 31;10(1):4967. doi:

Insert

hygR_gp41-1 N-intein + hygR_gp41-1 C-intein

Expression

Bacterial

Mutation

P254Q in hygR_gp41-1 N-intein

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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P16ΔS:S10-(MCS)-3xHA

Plasmid

#108183

Purpose

Binary vectors used for the constitutive expression of tripartite split-sfGFP components (with a 3xHA tag) in plants

Depositor

Tzu-Yin Liu

Publication

Liu et al Plant J. 2018 Feb 16. doi: 10.1111/tpj.1

Type

Empty backbone

Use

Synthetic Biology

Expression

Plant

Promoter

P16ΔS

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CD8bM-3

Plasmid

#40080

Depositor

Paula Kavathas

Publication

Thakral et al J Immunol. 2008 Jun 1;180(11):7431-42.

Insert

CD8bM-3

Expression

Mammalian

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only
pN1-CMV-Aff6_V18FdN-IntC-mCherryC-T2A-EGFP (C2)

Plasmid

#210725

Purpose

NIR optogenetic module for conditional protein splicing of split mCherry (C-part)

Depositor

Vladislav Verkhusha

Publication

Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

Insert

C2

Expression

Mammalian

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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SF-U7snRNA-CypA-E4

Plasmid

#190695

Purpose

Control vector expressing a U7-based antisense oligonucleotide targeting CypA exon 4

Depositor

Pim Pijnappel

Publication

van der Wal et al Mol Ther Nucleic Acids. 2017 Jun 16;7:90

Insert

U7 snRNA antisense CypA Exon 4

Use

Lentiviral

Expression

Mammalian

Promoter

U7 promoter

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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