We narrowed to 3,167 results for: tef
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Plasmid#64741PurposeYeast expression plasmidDepositorTypeEmpty backboneExpressionYeastAvailable sinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by
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p6347 MSCV-CMV-Flag-HA-Brd4-444-722
Plasmid#31353DepositorAvailable sinceOct. 20, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
p6348 MSCV-CMV-Flag-HA-Brd4 1047-1362
Plasmid#31354DepositorAvailable sinceSept. 28, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDS2-aurZ
Plasmid#70151PurposeFusarium graminearum AurZ encoded on CEN/ARS yeast shuttle vector with DS2 selection markerDepositorInsertaurZ
ExpressionYeastPromoterTefAvailable sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FRB-NLS-3HA-KanMX6
Plasmid#228022PurposeTo integrate TID-FRB-NLS fusion at yeast promoter; yeast BioID with KanMX6 markerDepositorInsertTurboID-FRB
TagsHA tagExpressionYeastAvailable sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFa6-TID-FKBP-NLS-3HA-KanMX6
Plasmid#228026PurposeTo integrate TID-FKBP-NLS fusion at yeast promoter; yeast BioID with KanMX6 markerDepositorInsertTurboID-FKBP
TagsHA tagExpressionYeastAvailable sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEasyG2_hph
Plasmid#184915PurposeTemplate to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.DepositorInsertgRNA scaffold
UseCRISPRExpressionBacterialAvailable sinceOct. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
RCP83
Plasmid#163466PurposeBackbone plasmid for insertion of the Saccharomyces cerevisiae promoter library and yeGFPDepositorInsertsPromoter fragment (Sharon2012_1922)
His3 minimal promoter
yeGFP
ExpressionBacterial and YeastAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKP4010
Plasmid#113336PurposeBeta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoterDepositorInsertsArtificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase
Tags3X-FLAG/8X-Gly linkerExpressionYeastAvailable sinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
pK3F-PYK1
Plasmid#85771PurposeN-ICE plasmid pK3F-PYK1DepositorInsertloxP-KanMX-loxP-PYK1p-3FLAG
UseCre/LoxExpressionYeastAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pITEu2
Plasmid#218282PurposeUse together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locusDepositorPublicationInsertHO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
ExpressionYeastAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJE13HD7
Plasmid#218281PurposeUse together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.DepositorPublicationInsertHO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
ExpressionYeastAvailable sinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJQMVAu7
Plasmid#262471PurposeOverexpressing HMG2K6R plasmid under the GAL promoter; used in combination with pJQMVAd2DepositorInsertLEU2(-235,-1)-PsbGAL2-HMG2K6R-TIDP1-LoxP-IsceIsite-PAgTEF-ble
ExpressionYeastAvailable sinceNov. 13, 2026AvailabilityAcademic Institutions and Nonprofits only -
pCas9-EcRT-Z3-Kan
Plasmid#232096PurposeYeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.DepositorInsertZ3 promoter and Z3EV transcription factor
UseCRISPRExpressionYeastPromoterZ3Available sinceJan. 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
HYG-TET-OFF – ubiquitin – LEU – 4FLAG
Plasmid#193315PurposeDoxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.DepositorInsertUbiquitin - Leucine - 4FLAG N-terminal tagging
Tags4FLAGExpressionBacterialAvailable sinceJan. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a- mECitrine-kanMX6
Plasmid#105150PurposeYeast gene targetingDepositorInsertmECitrine-kanMX6
TagsmECitrineExpressionBacterial and YeastMutationF64L, Q69M, A206KAvailable sinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCY 3030-02
Plasmid#36215DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertyEmCFP
UseYeast cassette for pcr and homologous recombinati…TagsyEpolylinker-yEmCFP-hphMX4Available sinceAug. 3, 2012AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pALD4 T4
Plasmid#161485PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T4_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (targeting T4 of pTHI11 promoter)
ExpressionYeastPromoterpALD4, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
THI VP64 pALD4 T5
Plasmid#161486PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pALD4_THI11_T5_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (targeting T5 of pTHI11 promoter)
ExpressionYeastPromoterpALD4, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
RIB VP64 R2
Plasmid#161494PurposeCRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_RIB1_R2_gRNA_MS2DepositorInsertsdCAS9
MS2-VP64
gRNA (Targeting R2 in the RIB1 promoter)
ExpressionYeastPromoterpGAP, pPOR1, and pTEF2Available sinceMarch 15, 2021AvailabilityAcademic Institutions and Nonprofits only