We narrowed to 19,334 results for: MUT
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Plasmid#123344PurposeNegative control mutant for Venus-cpVenus-FLARE-CKAR biosensor.DepositorInsertVenus-cpVenus-FLARE-CKAR(T/A)
Tags6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…ExpressionMammalianMutationTarget Thr residue in substrate domain mutated to…PromoterCMVAvailable SinceJuly 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
B-HypaSpCas9
Plasmid#126763PurposeExpression plasmid for human codon-opt. increased fidelity Blackjack-HypaSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HypaSpCas9.DepositorInsertB-HypaSpCas9
UseCRISPRTags3xFLAG and NLSExpressionMammalianMutationN692A; M694A; Q695A; H698A; amino acids 1005-1013…PromoterCBhAvailable SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-FLAG-B-eSpCas9
Plasmid#126772PurposeExpression of increased fidelity Blackjack-eSpCas9 in bacterial cells. Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than that of eSpCas9DepositorInsertB-eSpCas9
UseCRISPRTags3xFLAG, 6xHis, MBP, and NLSExpressionBacterialMutationK848A; K1003A; R1060A; amino acids 1005-1013 repl…PromoterT7Available SinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
AdMKP-3 S159AS197A
Plasmid#131523PurposeExpresses MKP-3 with S159A mutation and S197A mutation in mammalian cellsDepositorInsertMitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)
UseAdenoviralTagsNoMutationchanged Serine 159 to Alanine and Serine 197 to A…PromoterCMVAvailable SinceOct. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C423A-2xStrep
Plasmid#131111PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C423A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 423 muta…PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C399A-2xStrep
Plasmid#131112PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C399A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 399 muta…PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C393A-2xStrep
Plasmid#131113PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C393A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 393 muta…PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C355A-2xStrep
Plasmid#131114PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C355A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 355 muta…PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C344A-2xStrep
Plasmid#131115PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C344A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 344 muta…PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO-ORF66 C341A-2xStrep
Plasmid#131116PurposeExpresses KSHV ORF66 a.a. 1-429 (full length) C341A mutant with a C-terminal 2xStrep tag in mammalian cellsDepositorInsertORF66 (ORF66 Kaposi's Sarcoma Associated Herpesvirus (HHV-8))
Tags2xStrepExpressionMammalianMutationORF66 a.a. 1-429 (full-length); cysteine 341 muta…PromoterCMVAvailable SinceOct. 17, 2019AvailabilityAcademic Institutions and Nonprofits only