We narrowed to 4,220 results for: PRS
-
Plasmid#71789PurposeBrdU-incorporating plasmid with selectable marker HIS3DepositorAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only
-
pHAGE PGK-GFP-IRES-LUC-W
Plasmid#46793Purpose2nd generation lentiviral vectorDepositorInsertsUseLentiviralAvailable SinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNCS mOrange
Plasmid#91764PurposeBacterial expression of a fluorescent protein, monomeric Orange (mOrange)DepositorInsertmonomeric Orange
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialPromoterT7Available SinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMK425 (GAL-OsTIR1(F74A))
Plasmid#140657PurposeGAL-OsTIR1(F74A)DepositorInsertGAL-OsTIR1(F74A)
ExpressionYeastAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)
Plasmid#78231PurposeExpresses Cas9 protein, under the control of the TEF1 promoter, and CYC terminator. KanMX resistanceDepositorInsertCas9
ExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant pLV-SigmaR1-mNeonGreen
Plasmid#226569PurposeEncodes siRNA resistant SigmaR1 protein labeled with mNeonGreen (lentiviral vector)DepositorAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
CFP-Parkin
Plasmid#47560PurposeCFP tagged Parkin for colocalization studyDepositorAvailable SinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMXs-IP HA-Parkin
Plasmid#38248DepositorAvailable SinceAug. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMK198 (GAL-OsTIR1)
Plasmid#140655PurposeGAL-OsTIR1(WT)DepositorInsertOsTIR1(WT)
ExpressionYeastAvailable SinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNCS mStrawberry
Plasmid#91768PurposeBacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry)DepositorInsertmonomeric Strawberry
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialPromoterT7Available SinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pV1382
Plasmid#111436PurposeS. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatRDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
p306-BrdU-Inc
Plasmid#71792PurposeBrdU-incorporating plasmid with selectable marker URA3DepositorAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mCherry
Plasmid#226567PurposeEncodes siRNA resistant SigmaR1 protein labeled with mCherryDepositorAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZS157 CRISPEY RT/Cas9
Plasmid#114454PurposeYeast Integration Plasmid for galactose inducible Ec86-RT and SpCas9 expressionDepositorInsertSpCas9, Ec86-RT
UseCRISPRTagsSV40-NLSExpressionYeastMutationS. cerevisiae codon optimizedPromoterGAL1-GAL10Available SinceOct. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCSN075
Plasmid#166732PurposeSingle copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiaeDepositorInsertdCas12a-NLS-Mxi1
UseCRISPRTagsMxi1 and SV40 NLSExpressionYeastMutationE925APromoterS. cerevisiae TEF1Available SinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pV1326
Plasmid#111435PurposeS. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatRDepositorInsertCaCas9/sgRNA-BsmBI stuffer
UseCRISPRExpressionYeastAvailable SinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBK.079
Plasmid#187218PurposeExpresses Eco1 RT and Cas1 + Cas2DepositorInsertsRetron Eco1 Reverse Transcriptase
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterJ23115 and T7/lacAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPEPY-PF6-lacI
Plasmid#85589PurposeWork as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniaeDepositorInsertlacI
ExpressionBacterialPromoterPF6Available SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
M249-5'csr-1-Pgsy-1-luciferase-Bar-3'csr-1
Plasmid#89693PurposeLuciferase reporter plasmid targeted at csr-1 locusDepositorInsertgsy-1 promoter, luciferase
UseYeast episomal vectorTagsluciferasePromotergsy-1Available SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mNeonGreen R119A
Plasmid#226572PurposeEncodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
TagsmNeonGreenExpressionMammalianMutationR119AAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mNeonGreen D188A
Plasmid#226573PurposeEncodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
TagsmNeonGreenExpressionMammalianMutationD188AAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mNeonGreen F191A
Plasmid#226571PurposeEncodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
TagsmNeonGreenExpressionMammalianMutationF191AAvailable SinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDuRCC-K
Plasmid#81193PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease see depositor comments below., Please view…PromoterROX3 and SNP52pAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNCS tdTomato
Plasmid#91767PurposeBacterial expression of a fluorescent protein, tandem dimer Tomato (tdTomato)DepositorInserttandem dimer Tomato
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialPromoterT7Available SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
p415-PtrpC-Cas9-TtrpC-CYC1t
Plasmid#68059PurposeExpress humanized Cas9 under the control of trpC promoter and terminator from A. nidulans.DepositorInserthumanized Cas9
UseCRISPR; N. crassa, fungiExpressionYeastAvailable SinceOct. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
(189) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4)
Plasmid#160739PurposeExpresses a truncated OGT (4.5 TPRs) fused to a GFP nanobodyDepositorInsertHA-nGFP-(EAAAK)4-OGT(4)
TagsHA-TagExpressionMammalianPromoterT7/CMVAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
p405-BrdU-Inc
Plasmid#71791PurposeBrdU-incorporating plasmid with selectable marker LEU2DepositorAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-PEX3
Plasmid#51572PurposeConstitutively express peroxisome localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
TagsPEX3 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLS.405
Plasmid#187203PurposeExpresses wild-type retron Eco1 ncRNA + Eco1 RT, and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA (wildtype)
Cas1 + Cas2
UseSynthetic BiologyExpressionBacterialPromoterT7/LacAvailable SinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd3
Plasmid#98298PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters.DepositorInsertPRPL8B-ERG12-TNAT5-PSSB1-ERG8-TIDP1-PRPL3-MVD1-TIDP1-PYEF3-IDI1-TRPL15A
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGEX-parkin
Plasmid#227367PurposeFor bacterial expression of parkinDepositorInsertParkin (PRKN Human)
TagsGSTExpressionBacterialMutationCodon usage optimized for E.coli expression.Available SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
p404-BrdU-Inc
Plasmid#71790PurposeBrdU-incorporating plasmid with selectable marker TRP1DepositorAvailable SinceFeb. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-1_VNp-mNeongreen_CydAB-mCherry
Plasmid#182391PurposeBacterial expression of Vesicle Nucleating peptide-mNeongreen and cydAB-mCherry fusionsDepositorInsertsVNp-mNeongreen-His
cydAB-mCherry
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDuRCC-N
Plasmid#81194PurposeExpression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin ResistanceDepositorInsertsCas9
empty gRNA cassette
empty gRNA cassette
UseCRISPRTagsNTSExpressionYeastMutationPlease view depositor comments below and WT - cod…PromoterROX3 and SNP52pAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJT9R
Plasmid#98305PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p and Actinidia chinensis nerolidol/linalool synhtase AcNES1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3AcNES1>TRPL41B
ExpressionYeastAvailable SinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
BAS286 C terminal SF-GFP hygBR
Plasmid#74081PurposeS.pombe recoded superfolder GFP in universal C-terminal tagging plasmid, hygB resistancDepositorInsertsuper-folder GFP
Available SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLAREG
Plasmid#73044PurposeInducible expression of EGFP in yeast. Has dual-mode promoter with activation and repression capability for tuning gene expression in yeast.DepositorInsertsEGFP
LacI
ExpressionYeastPromoterGAL1 promoter and hybrid dual-mode promoter-see c…Available SinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSin-GFP-Fg
Plasmid#174307PurposeLentiviral expression of GFPDepositorAvailable SinceSept. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SENP8 (full length))
Plasmid#16361DepositorInserthuman SENP8 full length (SENP8 Human)
TagsHisExpressionBacterialMutationhuman SENP8 full length, see Depositor CommentsAvailable SinceDec. 19, 2007AvailabilityAcademic Institutions and Nonprofits only -
pVG1
Plasmid#111444PurposeUnified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempateDepositorInsertCaCas9/sgScADE2/stop-codon repair
UseCRISPRExpressionYeastAvailable SinceDec. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
ZJOM11
Plasmid#133637PurposeIntegration of GFP-TLG1 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast late golgi/early endosome.DepositorAvailable SinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
(424) pcDNA3.1(+)-HA-LaG6-(EAAAK)4-OGT(4.5, K852A)
Plasmid#168191PurposeHA-tagged GFP nanobody Lag6 (310 nM affinity) fused to an inactive truncated OGT of 4 TPRs with K852A mutationDepositorInsertHA-nLag6-(EAAAK)4-OGT(4,K852A)
TagsHA-TagExpressionMammalianPromoterT7/CMVAvailable SinceMay 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM09
Plasmid#135479PurposeSpizellomyces Hsp70 promoter driving expression of Hygromycin resistance fused to GFPDepositorInsertSpunHsp70pr
TagsGFP and hphExpressionBacterial and YeastPromoterSpun Hsp70prAvailable SinceMay 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
POT2-RFP
Plasmid#65318Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109AsAmp30E
Plasmid#123144PurposeArsenic sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-30arsR-t-ParsR-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, ParsR, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
(137) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4)
Plasmid#160740PurposeExpresses a truncated OGT (4.5 TPRs) fused to the EPEA nanobodyDepositorInsertHA-nEPEA-(EAAAK)4-OGT(4)
TagsHA-TagExpressionMammalianPromoterT7/CMVAvailable SinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPMVAd36
Plasmid#98302PurposeEnhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of consitutive promoters or CUP1 promoter.DepositorInsertPCUP1>ERG12>TNAT5-PTEF2>ERG8>TIDP1-TIDP1ExpressionYeastAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJT11
Plasmid#98308PurposeOverexpressing yeast farnesyl pyrophosphate synthase Erg20p mutant N127W and Citrus limon limonene synthase LIS1 under the control of GAL1/GAL2 promoterDepositorInsertTRPL3ClLIS1>TRPL41B
ExpressionYeastAvailable SinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pXWJ109HgAmp30E
Plasmid#123149PurposeMercury sensor with an optimized HrpRS-based amplifier. pSB3K3 carrying J109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-tDepositorInsertJ109-32merR-t-PmerT-30hrpR-30hrpS-t-PhrpLE-30gfp-t
UseSynthetic BiologyExpressionBacterialPromoterJ109, PmerT, PhrpLEAvailable SinceMay 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
BAS285 C terminal mKO2 hygBR
Plasmid#74080PurposeS.pombe recoded mKO2 in universal C-terminal tagging plasmid, hygB resistance.DepositorInsertmKO2
Available SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only