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Showing: 3851 - 3900 of 4075 results

  2. pCSN071

    Plasmid
    #166729
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-Mxi1-NLS
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166729-plasmid-map-sequence-id-332207

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  3. pCSN072

    Plasmid
    #166730
    Purpose
    Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS, Mxi1 and NLS, codon optimized for Saccharomyces cerevisiae
    Depositor
    Rene Verwaal
    Article
    Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312
    Insert
    dCas12a-NLS-Mxi1-NLS
    Use
    CRISPR
    Tags
    Mxi1 and SV40 NLS
    Expression
    Yeast
    Mutation
    E925A
    Promoter
    S. cerevisiae TEF1
    Available since
    June 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    166730-plasmid-map-sequence-id-332216

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  13. GFP-X2DM

    Plasmid
    #115571
    Purpose
    GFP-X2 where both of the consensus motif's lysines are mutated to arginine
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    Green Fluorescent Protein
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Two repeats of the Gcn5 consensus motif are tagge…
    Promoter
    ADH1
    Available since
    Oct. 29, 2018
    Availability
    Academic Institutions and Nonprofits only

  14. GFP-X2R2

    Plasmid
    #115570
    Purpose
    GFP-X2 where the second consensus motif's lysine is mutated to arginine
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    Green Fluorescent Protein
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Two repeats of the Gcn5 consensus motif are tagge…
    Promoter
    ADH1
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    115570-plasmid-map-sequence-id-226341

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  15. GFP-X2R1

    Plasmid
    #115569
    Purpose
    GFP-X2 where the first consensus motif's lysine is mutated to arginine
    Depositor
    Michael Downey
    Article
    Rossl et al J Biol Chem. 2019 Apr 19;294(16):6227-62
    Insert
    Green Fluorescent Protein
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Two repeats of the Gcn5 consensus motif are tagge…
    Promoter
    ADH1
    Available since
    Oct. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    115569-plasmid-map-sequence-id-226337

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  16. Hsh155 T178A R181A R182A R186A (N4A)

    Plasmid
    #111940
    Purpose
    This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the T178A R181A R182A R186A mutation
    Depositor
    Aaron Hoskins
    Article
    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d
    Insert
    Hsh155 T178A R181A R182A R186A (N4A)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    T178A R181A R182A R186A
    Promoter
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    111940-plasmid-map-sequence-id-213574

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  17. Hsh155 Q773A R775A R776A Q778A (C4A)

    Plasmid
    #111946
    Purpose
    This is a shuffle plasmid containing the HSH155 gene and the surrounding genomic regions. It bears a TRP marker for shuffle into a HSH155 deletion strain. It has the Q773A R775A R776A Q778A mutation
    Depositor
    Aaron Hoskins
    Article
    Carrocci et al RNA. 2018 May 11. pii: rna.065664.118. d
    Insert
    Hsh155 Q773A R775A R776A Q778A (C4A)
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Q773A R775A R776A Q778A
    Promoter
    Available since
    June 21, 2018
    Availability
    Academic Institutions and Nonprofits only
    111946-plasmid-map-sequence-id-213550

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  19. pTCW018

    Plasmid
    #83531
    Purpose
    Aromatic amino acid inducible expression of alpha pheromone, and pheromone inducible exression of an ARO7 RNA interference hairpin
    Depositor
    Claudia Vickers
    Article
    Williams et al Metab Eng. 2015 May;29:124-134. doi: 10.
    Insert
    pARO9-mf_2-pFUS1J2-ARO7i-CYC1t
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    pARO9/pFUS1J2
    Available since
    Oct. 18, 2016
    Availability
    Academic Institutions and Nonprofits only
    83531-plasmid-map-sequence-id-159463

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  22. pDuRCC-K

    Plasmid
    #81193
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Kan Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please see depositor comments below., Please view…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81193-plasmid-map-sequence-id-154419

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  29. pPFO*

    Plasmid
    #120400
    Purpose
    Expresses Perfringolysin O cholesterol sensor, has N-terminal His6-tag and three point mutations, A71T, C459A, and Y181A. Y181A prevents PFO's lytic activity at 4°C.
    Depositor
    Arun Radhakrishnan
    Article
    Das et al Proc Natl Acad Sci U S A. 2013 Jun 25;11
    Insert
    Mutant form of Perfringolysin O
    Use
    Tags
    His6 tag
    Expression
    Bacterial
    Mutation
    Amino acids 1-27 are deleted, starts at amino aci…
    Promoter
    Available since
    Jan. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    120400-plasmid-map-sequence-id-233255

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  42. pIMVAu1

    Plasmid
    #98301
    Purpose
    Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of galactose-inducible promoters; integrated into genome HMG2 locus; overexpressing HMG2 K6R.
    Depositor
    Claudia Vickers
    Article
    Peng et al Biotechnol Biofuels. 2017 Feb 21;10:43.
    Insert
    HMG2(-309,-153)-HIS3-TEFM1ACS2>TACS2-PGAL2> EfmvaE >TEBS1-PGAL7>HMG2K6A(1,292)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Sept. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    98301-plasmid-map-sequence-id-190182

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  48. pIMVAd40R

    Plasmid
    #98309
    Purpose
    Enhancing the lower-part mevalonate pathway; overexpressing yeast mevalonate pathway genes under the control of GAL promoters; integrated into ERG9 locus;
    Depositor
    Claudia Vickers
    Article
    Peng et al Metab Eng. 2018 Feb 19. pii: S1096-7176(
    Insert
    ERG9C-terminal-TURA3- PGAL7>MVD1>TPRM9-PGAL2>-ERG12>TNATTIDP1
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    98309-plasmid-map-sequence-id-182601

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Showing: 3851 - 3900 of 4075 results