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We narrowed to 4,300 results for: Pet2

Showing: 3851 - 3900 of 4300 results

  1. pJK281

    Plasmid
    #71137
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H59F mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-59 to phenylalanine; additional…
    Promoter
    T7
    Available Since
    Jan. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71137-plasmid-map-sequence-id-131036

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  2. pJK280

    Plasmid
    #71136
    Purpose
    Expression of Acetobacter aceti 1023 PurE (H89F mutant) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes histidine-89 to phenylalanine; additional…
    Promoter
    T7
    Available Since
    Jan. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    71136-plasmid-map-sequence-id-131035

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  3. pJK175

    Plasmid
    #71132
    Purpose
    Expression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase (class I PurE) in E. coli
    Depositor
    T. J. Kappock
    Article
    Constantine et al Biochemistry. 2006 Jul 11;45(27):8193-20
    Insert
    N5-carboxyaminoimidazole ribonucleotide mutase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    additional N-terminal methionine encoded by this …
    Promoter
    T7
    Available Since
    Jan. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    71132-plasmid-map-sequence-id-131032

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  4. pfMDH_Triple

    Plasmid
    #67733
    Purpose
    Expresses H303AR373AK381A mutant of mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli
    Depositor
    Justin Siegel
    Article
    Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:
    Insert
    Mannitol 2-Dehydrogenase from Pseudomonas fluorescens
    Use
    Tags
    6x-His tag
    Expression
    Bacterial
    Mutation
    Changed histidine 303 to alanine, arginine 373 to…
    Promoter
    T7
    Available Since
    Sept. 9, 2015
    Availability
    Academic Institutions and Nonprofits only

  5. pET:CACT:GFP

    Plasmid
    #60737
    Purpose
    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'CACT' between -11 and -8.
    Depositor
    Matthew Bennett
    Article
    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11
    Insert
    GFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available Since
    Nov. 5, 2014
    Availability
    Academic Institutions and Nonprofits only
    60737-plasmid-map-sequence-id-97723

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  7. Fast-GCaMP-RS09

    Plasmid
    #52649
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-RS09
    Use
    Tags
    Expression
    Mammalian
    Mutation
    L414T mutated from GCaMP3
    Promoter
    T7
    Available Since
    May 15, 2014
    Availability
    Academic Institutions and Nonprofits only

  8. Fast-GCaMP-RS05

    Plasmid
    #52646
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-RS05
    Use
    Tags
    Expression
    Mammalian
    Mutation
    M373Q mutated from GCaMP3
    Promoter
    T7
    Available Since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  9. Fast-GCaMP-RS08

    Plasmid
    #52648
    Purpose
    genetically encodable fluorescent calcium indicator protein to monitor neuronal activity
    Depositor
    Samuel Wang
    Article
    Sun et al Nat Commun. 2013 Jul 17;4:2170. doi: 10.
    Insert
    Fast-GCaMP-RS08
    Use
    Tags
    Expression
    Mammalian
    Mutation
    T412Q/L414T mutated from GCaMP3
    Promoter
    T7
    Available Since
    April 23, 2014
    Availability
    Academic Institutions and Nonprofits only

  10. DMTF1

    Plasmid
    #36895
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    DMTF1 (DMTF1 Human)
    Use
    Tags
    His tag with TEV cleavage
    Expression
    Bacterial
    Mutation
    contains Myb-like 1 domain, residues 220-274
    Promoter
    Available Since
    June 19, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  26. mEGFP-NbALFA

    Plasmid
    #159986
    Purpose
    For the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)
    Depositor
    Motoyuki Hattori
    Article
    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:
    Insert
    mEGFP-NbALFA
    Use
    Tags
    8histidine-HRV3C protease cleavage site
    Expression
    Bacterial
    Mutation
    3GS linker between mEGFP and NbALFA
    Promoter
    Available Since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    159986-plasmid-map-sequence-id-314744

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  27. mCherry-NbALFA

    Plasmid
    #159987
    Purpose
    For the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)
    Depositor
    Motoyuki Hattori
    Article
    Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:
    Insert
    mCherry-NbALFA
    Use
    Tags
    8histidine-HRV3C protease cleavage site
    Expression
    Bacterial
    Mutation
    3GS linker between mCherry and NbALFA
    Promoter
    Available Since
    Oct. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    159987-plasmid-map-sequence-id-314745

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  31. pBJG1 T7-8xHis-OGT

    Plasmid
    #154271
    Purpose
    Bacterial expression of full length human OGT. N-terminal T7_leader-8xHis tag followed by HRV3C protease site.
    Depositor
    Suzanne Walker
    Article
    Gross et al J Am Chem Soc. 2005 Oct 26;127(42):14588
    Insert
    O-GlcNAc Transferase (OGT Human)
    Use
    Tags
    8x His, HRV3C protease site, and T7 leader
    Expression
    Bacterial
    Mutation
    bacterial codon optimized
    Promoter
    Available Since
    Sept. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154271-plasmid-map-sequence-id-304821

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  34. pT22-GluCNZ-6His

    Plasmid
    #213504
    Purpose
    Designed for cytoplasmic E.coli overexpression of glucanase (β-1,3-glycosidase) for gentle yeast spheroblasting that unlike Zymolyase prevents proteolysis of intracellular components.
    Depositor
    John Denu
    Article
    Kuznetsov et al J Biol Chem. 2018 Aug 3;293(31):12271-12
    Insert
    Glucanase (glucan endo-1,3-beta-glucosidase)
    Use
    Tags
    6His tag
    Expression
    Bacterial
    Mutation
    1-36 Signal Sequence Deletion
    Promoter
    T7
    Available Since
    March 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    213504-plasmid-map-sequence-id-424932

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  38. AviTag-SpyTag003-MBP

    Plasmid
    #184227
    Purpose
    Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.
    Depositor
    Mark Howarth
    Article
    Vester et al Nat Commun. 2022 Jun 28;13(1):3714. doi:
    Insert
    AviTag-SpyTag003-MBP
    Use
    Tags
    AviTag, His6, SpyTag003, and Thrombin cleavage si…
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available Since
    June 29, 2022
    Availability
    Academic Institutions and Nonprofits only
    184227-plasmid-map-sequence-id-361606

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  48. pSUMO-YHRC

    Plasmid
    #54336
    Purpose
    Expression vector (pT7) for untagged expression as well as fusions to His6-SUMO or His6 tags. Designed for single or multiple fragment cloning by yeast homologous recombination.
    Depositor
    Claes Andréasson
    Article
    Holmberg et al Protein Expr Purif. 2014 Jun;98:38-45. d
    Type
    Empty backbone
    Use
    Tags
    6His-SUMO (yeast gene: SMT3)
    Expression
    Bacterial
    Mutation
    Promoter
    T7-LacO
    Available Since
    Aug. 1, 2014
    Availability
    Academic Institutions and Nonprofits only
    54336-plasmid-map-sequence-id-84530

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  50. pHYP-NPC-10H

    Plasmid
    #162789
    Purpose
    SARS-CoV-2 Nucleocapsid Protein expression in E.coli
    Depositor
    Ivan Vorobiev
    Article
    Kolesov et al PeerJ. 2022 Jan 7;10:e12751. doi: 10.771
    Insert
    Nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    10xHis
    Expression
    Bacterial
    Mutation
    Promoter
    T7lac
    Available Since
    Jan. 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    162789-plasmid-map-sequence-id-333793

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Showing: 3851 - 3900 of 4300 results