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Showing: 3851 - 3900 of 4184 results

  1. Kif3A-EGFP-SNAP

    Plasmid
    #229852
    Purpose
    Kif3A (aa1-342)-Kif1A neck linker(17aa) fused to Kin1 coiled coil (aa345-406)-EGFP-SNAP-his. This Kif3A was used to link an oligo to the motor using the SNAP tag for single molecule experiments
    Depositor
    William Hancock
    Insert
    Kif3A (Kif3a Mouse)
    Use
    Tags
    6xHis, EGFP, and SNAP-tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7 promoter
    Available since
    Feb. 7, 2025
    Availability
    Academic Institutions and Nonprofits only
    229852-plasmid-map-sequence-id-460709

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  5. E547* FL USP7

    Plasmid
    #229001
    Purpose
    E547-mutant of human USP7
    Depositor
    Irina Bezsonova
    Insert
    USP7 1-546 (USP7 Human)
    Use
    Tags
    6xHis cleavable with thrombin
    Expression
    Bacterial
    Mutation
    E547 mutated to stop codon
    Promoter
    T7
    Available since
    Jan. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    229001-plasmid-map-sequence-id-458328

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  13. SUMO CRYGS 16-177 Q16C

    Plasmid
    #217673
    Purpose
    N-terminally truncated human gamma S crystallin residues 16-177 with Q16C mutation for native chemical ligation
    Depositor
    Larry David
    Insert
    CRYGS (CRYGS Human)
    Use
    Tags
    6XHis and SUMO
    Expression
    Bacterial
    Mutation
    Glutamine 16 changed to Cysteine, and amino acids…
    Promoter
    T7
    Available since
    May 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217673-plasmid-map-sequence-id-433254

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  28. NmMetQ

    Plasmid
    #172111
    Purpose
    Expression of Methionine Binding Protein (MetQ) with signal sequence
    Depositor
    Douglas Rees
    Article
    Sharaf et al Elife. 2021 Aug 19;10. pii: 69742. doi:
    Insert
    Methionine Binding Protein
    Use
    Tags
    10x Histidine Tag and N.meningitidis signal seque…
    Expression
    Bacterial
    Mutation
    Changed Proline 22 to Alanine
    Promoter
    T7 Promoter
    Available since
    Oct. 29, 2021
    Availability
    Academic Institutions and Nonprofits only
    172111-plasmid-map-sequence-id-344824

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  30. pLyGo-Ec-4

    Plasmid
    #163130
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PhoA signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    July 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    163130-plasmid-map-sequence-id-338661

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  31. pLyGo-Ec-3

    Plasmid
    #163129
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding OmpA signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    May 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    163129-plasmid-map-sequence-id-332101

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  32. pLyGo-Ec-5

    Plasmid
    #163131
    Purpose
    pLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding DsBA signal peptide and the LyGo cassette (SapI-ccdB-SapI)
    Depositor
    Morten Norholm
    Article
    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    April 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    163131-plasmid-map-sequence-id-329722

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  45. pJK360

    Plasmid
    #71571
    Purpose
    Produces Acetobacter aceti 1023 acetyl-CoA:oxalate CoA-transferase (UctC) with Thr2>Ala mutant (UctC-T2A)
    Depositor
    T. J. Kappock
    Article
    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d
    Insert
    acetyl-CoA:oxalate CoA-transferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    changes threonine-2 to alanine
    Promoter
    T7
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71571-plasmid-map-sequence-id-130928

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  46. pJK385

    Plasmid
    #71572
    Purpose
    Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase with C-terminal His6 tag (AarCH6)
    Depositor
    T. J. Kappock
    Article
    Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d
    Insert
    succinyl-CoA:acetate CoA-transferase
    Use
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Jan. 19, 2016
    Availability
    Academic Institutions and Nonprofits only
    71572-plasmid-map-sequence-id-130931

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Showing: 3851 - 3900 of 4184 results