We narrowed to 5,560 results for: Chia

1. pST50Trc3-DHFRSTR

   Plasmid

   #63986

   Purpose

   Position 3 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   Strep II (STR)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pST50Trc3-STRDHFR

   Plasmid

   #63973

   Purpose

   Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   Strep II (STR)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMAT10

   Plasmid

   #112591

   Purpose

   Used for expression of protein in E. coli as Thrombin cleavable N-termina 6His-tag-MBP fusions. Like pMAT9, but without the second BamHI site in the polylinker

   Depositor

   Johan Peränen, Marko Hyvönen

   Article

   Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

   Type

   Empty backbone

   Use

   Tags

   6His-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pST50Trc4-FLAGHISNDHFR

   Plasmid

   #64001

   Purpose

   Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   6xHis & FLAG; TEV cleavable

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pST50Trc4-FLAGDHFR

   Plasmid

   #63992

   Purpose

   Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pST50Trc3-HISDHFR

   Plasmid

   #63971

   Purpose

   Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Dec. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pST50Trc4-DHFRSTR

   Plasmid

   #64004

   Purpose

   Position 4 transfer plasmid for pST44 polycistronic plasmid suite; C-term single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   Strep II (STR)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pST50Trc4-FLAGNDHFR

   Plasmid

   #63997

   Purpose

   Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   FLAG; TEV cleavable

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET21a-AcrR

   Plasmid

   #51148

   Depositor

   Christopher Voigt

   Article

   Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

   Insert

   AcrR
   

   Use

   Synthetic Biology

   Tags

   6x-his

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pST50Trc2-FLAGDHFR

    Plasmid

    #63956

    Purpose

    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term non-cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Dec. 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pST50Trc2-FLAGHISNDHFR

    Plasmid

    #63965

    Purpose

    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable double tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

    Depositor

    Song Tan

    Article

    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

    Type

    Empty backbone

    Use

    Tags

    6xHis & FLAG; TEV cleavable

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDEST17_A1LigN_EC

    Plasmid

    #22296

    Depositor

    Oscar Millet

    Article

    Tadeo et al PLoS Biol. 2009 Dec;7(12):e1000257. doi:

    Insert

    A1 domain NAD+ dependent DNA ligase N
    

    Use

    Tags

    His tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 5, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pSF18

    Plasmid

    #222593

    Purpose

    BB2 ZWF1 tet-on overexpression cassette

    Depositor

    Matthias Steiger

    Article

    Matthias et al PLOS One

    Insert

    ZWF1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Internal BsaI and BpiI sites removed from insert

    Promoter

    tet-on

    Available since

    March 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pIA036

    Plasmid

    #200425

    Purpose

    amyE::(Ppen-lacIΔ11-bsdronPA cm). PALM integration vector includes codon optimized dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.

    Depositor

    Rut Carballido-López

    Article

    Altinoglu et al bioRxiv 2023

    Insert

    LacI
    

    Use

    Tags

    bsdronPA

    Expression

    Bacterial

    Mutation

    Promoter

    Ppen

    Available since

    Aug. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGU08

    Plasmid

    #212149

    Purpose

    Plasmid expressing catalase-peroxidase and D-amino acid dehydrogenase under constitutive promoter PgyrA control. Best E. coli glycine utilization plasmid.

    Depositor

    Kang Zhou

    Insert

    catalase-peroxidase, D-amino acid dehydrogenase (katG katG: E. coli, dadA6: Cupriavidus necator)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PgyrA

    Available since

    March 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCYP10

    Plasmid

    #212843

    Purpose

    Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Insert

    cytochrome P450
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PcymRC

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCYP13

    Plasmid

    #212846

    Purpose

    Expresses the Acidovorax avenae subsp. Avenae LRD-modifying cytochrome P450 enzyme

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Insert

    cytochrome P450
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PcymRC

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. INT_Cargo_PgyrA_katG_dadA6

    Plasmid

    #212142

    Purpose

    Cargo plasmid for integrating PgyrA expression cassette of catalase-peroxidase and D-amino acid dehydrogenase into the genome. Plasmid can be removed by incubating cells at 37 C.

    Depositor

    Kang Zhou

    Insert

    catalase-peroxidase, D-amino acid dehydrogenase (katG katG: E. coli, dadA6: Cupriavidus necator)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PgyrA

    Available since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLRD20

    Plasmid

    #208271

    Purpose

    Production of 13R-manoyl oxide in E. coli MEV20

    Depositor

    Christopher Voigt

    Article

    Lin et al Nature Catalysis. 6, 1016–1029 (2023). h

    Inserts

    tHMGR
    abCAS
    cfTPS3
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    abCAS(D537A)

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL0_101 [PnptII]

    Plasmid

    #199002

    Purpose

    Level 0 part

    Depositor

    Charlie Gilbert, Nili Ostrov, Cultivarium Tools

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    PnptII
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge