We narrowed to 165,528 results for: addgene

1. DCAF1:PBC020-D05:C246337

   Plasmid

   #183974

   Purpose

   Baculovirus expression for structure determination. May not contain entire coding region of gene.

   Depositor

   Cheryl Arrowsmith

   Insert

   DCAF1:PBC020-D05:C246337 (DCAF1 Human)
   

   Use

   Baculovirus expresssion

   Tags

   6xHis-TEV and MHHHHHHSSGRENLYFQG

   Promoter

   Polyhedrin

   Available Since

   May 10, 2022

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. 7TC

   Plasmid

   #24315

   Depositor

   Roel Nusse

   Article

   ten Berge et al Cell Stem Cell. 2008 Nov 6. 3(5):508-18.

   Insert

   7xTcf-mCherry
   

   Use

   Lentiviral

   Available Since

   April 5, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. 5KO9 (HMCES)

   Plasmid

   #101877

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   HMCES (HMCES Human)
   

   Tags

   6x His (modified C-term tag is AENLYFQSHHHHHH)

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 30, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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4. pLV hUbC- VP64-dCas9-VP64-T2A- GFP(No LoxP sites)

   Plasmid

   #66708

   Purpose

   expresses VP64-dCas9-VP64, same plasmid as Addgene 59791 but with LoxP sites removed

   Depositor

   Charles Gersbach

   Insert

   S.Pyogenes VP64-dCas9-VP64
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   VP64

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available Since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSADdeltaG-BFP

   Plasmid

   #32639

   Depositor

   Edward Callaway

   Article

   Osakada et al Neuron. 2011 Aug 25;71(4):617-31.

   Insert

   BFP
   

   Use

   Rabies virus

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 16, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNM3725

   Plasmid

   #154113

   Purpose

   UAS 11X ∆pes-10 promoter driving GFP-C1 in an RCME vector

   Depositor

   Michael Nonet

   Article

   Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

   Insert

   UAS 11X::∆pes-10::GFP-C1::tbb-2 3'
   

   Use

   Rmce

   Expression

   Worm

   Promoter

   not applicable

   Available Since

   July 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDD379

   Plasmid

   #91834

   Purpose

   SapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffold

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Worm

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. AMPKA2

   Plasmid

   #36194

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   AMPKA2 (PRKAA2 Human)
   

   Tags

   His tag with thrombin cleavage

   Expression

   Bacterial

   Mutation

   contains KINASE DOMAIN only

   Available Since

   Aug. 8, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pICH-roGFP2-synORP1

   Plasmid

   #191680

   Purpose

   Ti plasmid pICH86988 harbouring roGFP2-synORP1 for H2O2 detection in plant cells.

   Depositor

   Philip Mullineaux

   Article

   Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

   Insert

   rredox(roGFP2-synORP1
   

   Expression

   Plant

   Promoter

   CaMV 35S

   Available Since

   March 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. VV063: 1xCox8 - superecliptic pHluorin in fck

    Plasmid

    #58500

    Purpose

    Expresses the pH sensor superecliptic pHluorin in the mitochondria of mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    1xCox8 - Superecliptic pHluorin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    a-CamKII

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. JAK3 (JAK3A-c023)

    Plasmid

    #98229

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    JAK3A (JAK3 Human)
    

    Use

    Baculovirus

    Tags

    His-6-TEV

    Mutation

    pfam00069:Pkinase 822-1091

    Promoter

    polyhedrin

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. 6CCR (GID4)

    Plasmid

    #119960

    Purpose

    Bacterial expression for structure determination

    Depositor

    Cheryl Arrowsmith

    Insert

    GID4 (C17orf39 Human)
    

    Tags

    6xHIS, thrombin,SUMO and TEV cleavage

    Expression

    Bacterial

    Mutation

    contains amino acids 116-300

    Promoter

    T7

    Available Since

    Jan. 8, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. VV064: 1xCox8 - ratiometric pHluorin in fck

    Plasmid

    #58502

    Purpose

    Expresses the pH sensor ratiometric pHluorin in the mitochondria of mammalian cells

    Depositor

    Adam Cohen

    Article

    Venkatachalam et al Biophys J. 2014 Oct 7;107(7):1554-63. do

    Insert

    1xCox8 - Ratiometric pHluorin
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    a-CamKII

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNM3814

    Plasmid

    #154122

    Purpose

    mec-4 promoter driving tet ON rtetR-L-QFAD in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    mec-4p::rtetR-L-QFAD::act-4 '3
    

    Use

    Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. 5T5G (SETD8)

    Plasmid

    #101886

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    SETD8 (KMT5A Human)
    

    Tags

    6xHis, TEV

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 30, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pDD121

    Plasmid

    #91833

    Purpose

    Cas9 expression in C. elegans

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Worm

    Mutation

    Codon optimized and with synthetic introns for C.…

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiGuide-BC-start

    Plasmid

    #127168

    Purpose

    Entry plasmid for two-step guide-barcode cloning

    Depositor

    Paul Blainey

    Article

    Feldman et al Cell. 2019 Oct 17;179(3):787-799.e17. do

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    N/A

    Available Since

    June 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGlnA-Ypet

    Plasmid

    #98278

    Purpose

    mammalian expression of GlnA-Ypet

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    GlnA
    

    Tags

    Ypet

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNM3724

    Plasmid

    #154112

    Purpose

    tetO 7X ∆pes-10 promoter driving GFP-C1 in an RCME vector

    Depositor

    Michael Nonet

    Article

    Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

    Insert

    tetO 7X::∆pes-10::GFP-C1::tbb-2 3'
    

    Use

    Rmce

    Expression

    Worm

    Promoter

    not applicable

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. p6VP1

    Plasmid

    #24725

    Depositor

    Christopher Buck

    Article

    Schowalter et al Cell Host Microbe. 2010 Jun 25;7(6):509-

    Insert

    VP1 from Human Polyomavirus 6
    

    Expression

    Mammalian

    Available Since

    June 21, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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