We narrowed to 13,284 results for: sequence
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Plasmid#163137PurposepLyGo cloning vector for a sequence of interest (LPMO) in Bacillus subtilis. Vector encoding the AmyL signal peptide and the LyGo casette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterPamyL-PamyQ-PcryIIIA-cryIIIAstab49Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only
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pSBbi_RP_2XMTS-MicroID2-FLAG
Plasmid#188524PurposeEncodes the biotin proximity labeling enzyme MicroID2 with a 2X mitochondrial targeting sequence (MTS) for expression and stable integration into mammalian cellsDepositorInsert2XMTS-MicroID2
UseTransposon mediated gene integrationTagsFLAGExpressionMammalianPromoterEF1a/RPBSAAvailable SinceSept. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWPT-/GCCACC-mEGFP-IRES-mCherry
Plasmid#49235PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianPromoterEF1-shortAvailable SinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-LifeAct-FRB-Venus-173-N
Plasmid#87616PurposeFRB-tagged N-terminal Venus (YFP) fragment for BiFC; localizes to actin cytoskeleton.DepositorInsertLifeAct-FRB-Venus173-N
Tags17-aa sequence from Abp140 (aka "Lifeact&quo…ExpressionMammalianPromoterCMVAvailable SinceFeb. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
pUC19/N-mChe
Plasmid#183159PurposePlant transient expression vector containing mCherry tag. Unique nucleotide sequences Uc and Ud for cloning at N terminus of mCherry.DepositorTypeEmpty backboneExpressionPlantAvailable SinceApril 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-HaloTag
Plasmid#66993PurposeG protein alpha-s internally tagged with HaloTag and EE epitopeDepositorInsertG-alpha-s-EE-HaloTag (Gnas Rat)
TagsHaloTag flanked by SGGGGSGGGGS on each side was i…ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable SinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCIB1(deltaNLS)-pmGFP
Plasmid#28240PurposeExpresses CIB1 fused to plasma membrane-localized EGFPDepositorInsertCIB1(deltaNLS)-pmGFP (CIB1 Mustard Weed)
TagsEGFPExpressionMammalianMutationNLS sequences mutatedAvailable SinceMarch 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-dInPAkt(R23/25A)
Plasmid#181835PurposePhospholipid binding-deficient Lyn-dInPAkt negative-control plasmid.DepositorInsertLyn-dInPAkt
TagsN-terminal targeting sequence from Lyn kinase, dd…ExpressionMammalianMutationArginines 23 and 25 in PH domain mutated to alani…PromoterCMVAvailable SinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRvTUB-mEGFP-NLS
Plasmid#205011PurposeThe vector contains 1kbp of the upstream and downstream regions of the tubulin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the tubulin gene from R. varieornatus
UseTardigrade expressionAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-mios in pRK5
Plasmid#154212PurposeN-terminal HA-tagged Mios cDNA expression vector for transfectionsDepositorInsertMios
TagsHA tagExpressionMammalianMutationcDNA sequence has been codon optimized using huma…PromoterCMVAvailable SinceNov. 19, 2020AvailabilityAcademic Institutions and Nonprofits only