161,256 results

1. EF1a mCherry

   Plasmid

   #129431

   Purpose

   expression of mCherry from EF1a promoter

   Depositor

   Oskar Laur

   Insert

   mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-TO-hNGN2-BSD-mApple

   Plasmid

   #182308

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into glutamatergic neurons via NGN2 expression, bsd selection, mApple

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   hNGN2 (NEUROG2 Human)
   bsd-T2A-mycNLS-mApple
   

   Use

   Tags

   T2A-mycNLS-mApple

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and TRE3G

   Available since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDH-EF1-FHC

   Plasmid

   #64874

   Purpose

   lentiviral vector with Flag, HA tags

   Depositor

   Richard Wood

   Article

   Yousefzadeh et al PLoS Genet. 2014 Oct 2;10(10):e1004654.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Flag and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. iBE4max YE1 NG CLYBL

   Plasmid

   #174570

   Purpose

   doxycycline inducible BE4max YE1 NG expression for human CLYBL locus knock-in

   Depositor

   Mathew Garnett

   Article

   Coelho et al Cancer Cell. 2023 Feb 13;41(2):288-303.e

   Insert

   BE3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tet ON

   Available since

   Jan. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti6.3 GCN1-3xFlag_IRES-iRFP

   Plasmid

   #198388

   Purpose

   GCN1 expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   GCN1 (GCN1 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pST50Trc3-HISNDHFR

   Plasmid

   #63976

   Purpose

   Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control

   Depositor

   Song Tan

   Article

   Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9

   Type

   Empty backbone

   Use

   Tags

   6xHis; TEV cleavable

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pT7-VP3SA11

   Plasmid

   #89164

   Purpose

   Express Rotavirus SA11 VP3 in mammalian cells

   Depositor

   Takeshi Kobayashi

   Article

   Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

   Insert

   Rotavirus SA11 VP3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   T7 promotor

   Available since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRK5-HA-YFP-Raptor K840R

   Plasmid

   #204418

   Purpose

   Acetylation mutant for human raptor

   Depositor

   David Rubinsztein

   Article

   Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

   Insert

   Regulatory Associated Protein Of MTOR, Complex 1
   

   Use

   Tags

   HA and YFP

   Expression

   Mammalian

   Mutation

   Lysine 840 to Arginine (K840R)

   Promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5-HA-YFP-Raptor K1097R

   Plasmid

   #204417

   Purpose

   Acetylation mutant for human raptor

   Depositor

   David Rubinsztein

   Article

   Son et al Cell Metab. 2019 Jan 8;29(1):192-201.e7.

   Insert

   Regulatory Associated Protein Of MTOR, Complex 1
   

   Use

   Tags

   HA and YFP

   Expression

   Mammalian

   Mutation

   Lysine 1097 to Arginine (K1097R)

   Promoter

   Available since

   Nov. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. PB-U6insert-EF1puro

    Plasmid

    #104537

    Purpose

    A piggybac vector with puromycin resistance and a U6 promoter that allows cloning of guide RNAs

    Depositor

    George Church

    Article

    Kalhor et al Science. 2018 Aug 9. pii: science.aat980

    Type

    Empty backbone

    Use

    CRISPR, Mouse Targeting, and Synthetic Biology ; …

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    March 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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