We narrowed to 13,284 results for: sequence
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Plasmid#207535PurposeHomologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
TagsHaloTagExpressionMammalianPromoterEndogenousAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
StrKDEL-IRES_mScarleti_Sec23A
Plasmid#117273PurposeExpresses an Str-KDEL Hook for the RUSH system and separately mScarleti-Sec23ADepositorInsertsTagsmScarlet-iExpressionMammalianMutationHuman Sec23A; Sequencing showed a mutation in Sec…PromoterCMV and IRESAvailable SinceJan. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-GGG-ELP(120nm)-Cys
Plasmid#91572PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertGGG-ELP(120nm)-Cys
TagsCysteine and Sortase-TagExpressionBacterialPromoterT7Available SinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFK210B-AtMIR390a-B/c
Plasmid#51777PurposePlant expression vector (35S) for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursorDepositorInsertAtMIR390a-B/c
UsePlant expressionMutationA. thaliana MIR390a precursor sequence including …Available SinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Lyn-RAB-EKARev
Plasmid#118477PurposeddRFP-based single-color ERK biosensor targeted to the plasma membrane.DepositorInsertLyn-RAB-EKARev
TagsN-terminal targeting sequence from Lyn-kinaseExpressionMammalianPromoterCMVAvailable SinceMay 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCLV3Cas-LT1
Plasmid#210549PurposeBinary vector expressing the Lineage Tracing system. Cas9 expression driven by CLV3 promoter. LT1 reporterDepositorInsertCLV3p::spCas9::PEA3At::U6p::AAVS1gRNA::pHTR5::LT1::mCherrySSM
ExpressionPlantMutation2bp insertion after AAVS1 target sequence that di…Available SinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
4A3.PBAD-repL(WT).J23115-gfp
Plasmid#197872PurposeArabinose induced expression of wildtype repL gene, which triggers DNA replication in cis and in trans, weak level of constitutive GFP expressionDepositorInsertspart kilA/repL
gfp
ExpressionBacterialMutationcontains only the 24 bp of 3'-end of kilA ge…PromoterBba_J23115 and PBADAvailable SinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Crimson)
Plasmid#140162PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Crimson-FKBP(LV)(C22V)
TagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianPromoterEF-1aAvailable SinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV Ef1a DIO GeNL_SS hGtACR1
Plasmid#233511PurposeLuciferase opsin fusion protein (luminopsin) for bioluminescent optogeneticsDepositorInsertsmNeonGreen
ISN-IE
hGtACR1
UseAAVTagsGaussia luciferase signal sequenceAvailable SinceApril 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pRvUBC-mEGFP-NLS
Plasmid#205010PurposeThe vector contains 1kbp of the upstream and downstream regions of the ubiquitin c gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the ubiquitin c gene from R. varieornatus
UseTardigrade expressionAvailable SinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only