We narrowed to 10,340 results for: SEC

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IBMc180

Plasmid

#161942

Purpose

Carries Golden Gate substitution insert for acVHH-assisted bisection mapping

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1A3-BbsI-10aa-acVHH-T-cat-PBAD(SapI)-RiboJ-BCD22-acVHH-10aa-SapI

Expression

Bacterial

Promoter

PBAD

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pUASTLOTattB_vhhGFP4::Nrv1::tagBFP

Plasmid

#163925

Purpose

Expression of GrabFP-B-extracellular under control of UAS or LOP enhancer

Depositor

Markus Affolter

Article

Harmansa et al Elife. 2017 Apr 11;6. doi: 10.7554/eLife

Insert

Fusion of vhhGPF4 (extracellular) nanobody with Nrv1 protein and tagBFP fluorophor

Use

Cre/Lox

Tags

tagBFP

Expression

Insect

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCTCON2-BXG-altTAG2

Plasmid

#158134

Purpose

Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

Depositor

James Van Deventer

Article

Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

Insert

BFP-GFP fragment

Expression

Yeast

Mutation

Cloned in a TAG codon at the second serine residu…

Promoter

GAL1-10

Available Since

Jan. 12, 2021

Availability

Academic Institutions and Nonprofits only

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IDA3::HA exon2 (WS13)

Plasmid

#126874

Purpose

p3xHA inserted within second exon of IDA3. Includes ampicilin and hygromycin resistance.

Depositor

Winfield Sale

Article

Hunter et al Mol Biol Cell. 2018 Apr 15;29(8):886-896

Insert

IDA3

Use

Other

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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IDA3::NeonGreen exon2 (WS15)

Plasmid

#126876

Purpose

NeonGreen-tag embedded in the second exon of IDA3. Includes ampicilin and hygromycin resistance.

Depositor

Winfield Sale

Article

Hunter et al Mol Biol Cell. 2018 Apr 15;29(8):886-896

Insert

IDA3

Use

Other

Available Since

Aug. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCASP-SptP92-TD-HilA

Plasmid

#153331

Purpose

Triggers secretion of SptP92 fused to the HA4-7c12 Tandem Monobody from Salmonella upon induction with arabinose

Depositor

Josef Penninger

Article

Chabloz et al Commun Biol. 2020 Jul 3;3(1):342. doi: 1

Insert

SptP92-HA4-7c12 Tandem Monobody

Use

Salmonella expression

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pBz173_CsoS2_N2_GFP

Plasmid

#140853

Purpose

His-tagged second N-peptide of NTD fused to Superfolder GFP ([N2]-GFP) used for BLI, pET-14b backbone

Depositor

David Savage

Article

Oltrogge et al Nat Struct Mol Biol. 2020 Mar;27(3):281-

Insert

CsoS2 NTD repeat 2 fused to GFP

Tags

Hexahistidine tag, Strep-Tag II, and Superfolder …

Expression

Bacterial

Promoter

T7

Available Since

May 20, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP29[dCas9]-1A(lacZ)

Plasmid

#126979

Purpose

Level 1 vector. With lacZ as alternative cloning reporter.OriV 9 (pBBR322/ROP; medium copy number). Constitutive dCas9 in downstream secondary site.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

Cas9 catalytically dead

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pC-KIT3

Plasmid

#118985

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Expression

Mammalian

Mutation

K2-mutSP-K1

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pC-KIT7

Plasmid

#118989

Purpose

Mutational dissection of the G rich region of the c-kit promoter

Depositor

Bjorn Hogberg

Article

Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

Insert

c-kit proto-oncogene {promoter} (KIT Human)

Expression

Mammalian

Mutation

K2-mutSP-mutK1

Available Since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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