163,584 results

1. pEGFP(N3)-PP1beta

   Plasmid

   #44223

   Depositor

   Laura Trinkle-Mulcahy, Angus Lamond

   Article

   Trinkle-Mulcahy et al J Cell Sci. 2001 Dec;114(Pt 23):4219-28.

   Insert

   PP1B (PPP1CB Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-28b-T7-bLbCas12a-NLS(nucleoplasmin)-6xHis (RTW645)

   Plasmid

   #114070

   Purpose

   T7 promoter bacterial expression plasmid for bacterial codon optimized LbCas12a with C-terminal NLS and His-tag

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   bacteria codon optimized LbCas12a with C-terminal NLS and His-tag
   

   Use

   Tags

   NLS(nucleoplasmin) and 6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRL092 pattB_cryaa:Venus_MCS

   Plasmid

   #213513

   Purpose

   attB-containing vector backbone, for use in restriction enzyme- or PCR-based cloning

   Depositor

   Christian Mosimann

   Article

   Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

   Type

   Empty backbone

   Use

   Zebrafish expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLSSmOrange-mKate2 caspase-3 biosensor

   Plasmid

   #37132

   Depositor

   Vladislav Verkhusha

   Article

   Shcherbakova et al J Am Chem Soc. 2012 May 9;134(18):7913-2

   Insert

   LSSmOrange-DEVD-mKate2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   A44V/F84L/W145M/I165D/M167L/G202D compared to mOr…

   Promoter

   CMV

   Available Since

   Sept. 17, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLVX-TetOne-Puro-PSMD4

   Plasmid

   #202666

   Purpose

   Tet-ON 3rd generation expression vector with inducible expression of PSMD4 and a puromycin selection cassette

   Depositor

   Jason Sheltzer

   Article

   Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

   Insert

   proteasome 26S subunit ubiquitin receptor, non-ATPase 4 (PSMD4 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAV-FLEX-FLIM-AKAR

   Plasmid

   #60445

   Purpose

   Expresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in a Cre dependent manner in an AAV backbone

   Depositor

   Bernardo Sabatini

   Article

   Chen et al Front Pharmacol. 2014 Apr 2;5:56. doi: 1

   Insert

   FLIM-AKAR
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   Oct. 22, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAAV-CAG-flex-iGluSnFR4f-PDGFR-WPRE

   Plasmid

   #234448

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and fast deactivation kinetics (4f = fast); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4f-PDGFR
   

   Use

   AAV and Cre/Lox

   Tags

   IgK chain and Myc epi tag-PDGFR TM Domain

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. V5-LOV-Turbo-ERM

   Plasmid

   #199664

   Purpose

   expresses LOV-Turbo on the mammalian ER membrane, lentiviral vector

   Depositor

   Alice Ting

   Article

   Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

   Insert

   LOV-Turbo
   

   Use

   Lentiviral

   Tags

   SEC61B and V5

   Expression

   Mammalian

   Mutation

   Promoter

   TRE3G

   Available Since

   May 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEGFP-MRLC1

   Plasmid

   #35680

   Depositor

   Tom Egelhoff

   Article

   Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

   Insert

   MRLC1 (MYL9 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 24, 2012

   Availability

   Academic Institutions and Nonprofits only

10. TFORF0172

    Plasmid

    #142551

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ONECUT2 (ONECUT2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    June 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge