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Showing: 1561 - 1580 of 8297 results

  7. pT-GEM(0)

    Plasmid
    #62891
    Purpose
    Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons
    Depositor
    Benjamin White
    Article
    Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:
    Type
    Empty backbone
    Use
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    62891-plasmid-map-sequence-id-103965

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  8. pDuRCC-N

    Plasmid
    #81194
    Purpose
    Expression of Cas9 and two gRNA cassettes in S. cerevisiae; Nourseothricin Resistance
    Depositor
    Eckhard Boles
    Article
    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.
    Inserts
    Cas9
    empty gRNA cassette
    empty gRNA cassette
    Use
    CRISPR
    Tags
    NTS
    Expression
    Yeast
    Mutation
    Please view depositor comments below and WT - cod…
    Promoter
    ROX3 and SNP52p
    Available since
    Sept. 23, 2016
    Availability
    Academic Institutions and Nonprofits only
    81194-plasmid-map-sequence-id-160426

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  9. pGS1222

    Plasmid
    #63791
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eGFP Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eGFP-Twin-Strep tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eGFP-Twin-Strep tag
    Expression
    Mutation
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63791-plasmid-map-sequence-id-106478

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  10. pDOC-GG

    Plasmid
    #149377
    Purpose
    Chassis plasmid for building mutagenesis cassettes by Golden Gate assembly to form a donor plasmid for recombineering by Gene Doctoring
    Depositor
    Mark Pallen
    Article
    Thomson et al BMC Biotechnol. 2020 Oct 7;20(1):54. doi
    Inserts
    Fragment containing a kanamycin resistance cassette
    Fragment containing pMB1 origin of replication and an I-SceI recognition sequence
    Fragment containing a lacZ⍺ expression cassette flanked by BsaI sites
    Fragment containing an I-SceI recognition sequence
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    149377-plasmid-map-sequence-id-308794

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  14. pGS1011

    Plasmid
    #63783
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal Strep tag II followed by TRP1 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    Strep-tag II followed by TRP1 selection marker
    Use
    E. coli cloning vector
    Tags
    Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63783-plasmid-map-sequence-id-106469

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  15. pCIFR-3

    Plasmid
    #233295
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    msfGFP
    Use
    Tags
    Expression
    Mutation
    Promoter
    p14G
    Available since
    April 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    233295-plasmid-map-sequence-id-468475

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  16. pCIFR-8

    Plasmid
    #227666
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Am) used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Article
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    AmR
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227666-plasmid-map-sequence-id-458899

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  17. CMV-10xQb oligo-library based

    Plasmid
    #158201
    Purpose
    Cassette of 10 different binding sites with high affinity to QB phage coat protein, as extracted from the oligo pool experiment.
    Depositor
    Roee Amit
    Article
    Katz et al Nat Commun. 2021 Mar 11;12(1):1576. doi:
    Insert
    10 binding sites of QB based on oligo library experiment
    Use
    Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    March 11, 2021
    Availability
    Academic Institutions and Nonprofits only
    158201-plasmid-map-sequence-id-311814

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  18. pGS1623

    Plasmid
    #63795
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal tDimer2 Twin-Strep tag followed by HIS5 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    tDimer2-Twin-Strep tag followed by HIS5
    Use
    E. coli cloning vector
    Tags
    tDimer2-Twin-Strep
    Expression
    Mutation
    Promoter
    none
    Available since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    63795-plasmid-map-sequence-id-110439

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  19. pGS1322

    Plasmid
    #63792
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eCFP Twin-Strep tag followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eCFP-Twin-tag followed by URA3
    Use
    E. coli cloning vector
    Tags
    eCFP-Twin-tag
    Expression
    Mutation
    Promoter
    none
    Available since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63792-plasmid-map-sequence-id-106481

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  20. pGS1112

    Plasmid
    #63784
    Purpose
    Vector to create a cassette for homologous recombination in S. cerevisiae; adds a C-terminal eSapphire Strep-tag II followed by URA3 selection marker
    Depositor
    Monika Golas, Bjoern Sander
    Article
    Rai et al Mol Biotechnol. 2014 Jun 27.
    Insert
    eSapphire-Strep-tag II followed by URA3
    Use
    E. coli cloning vector
    Tags
    Sapphire-Strep-tag II
    Expression
    Mutation
    Promoter
    none
    Available since
    April 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    63784-plasmid-map-sequence-id-106474

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Showing: 1561 - 1580 of 8297 results