We narrowed to 5,067 results for: codon optimized

1. SpCas9-NRRH

   Plasmid

   #198540

   Purpose

   Expresses human codon-optimized SpCas9-NRRH and blasticidin resistance: EFS promoter-SpCas9-NRRH-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRRH
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SpCas9-NRCH

   Plasmid

   #198542

   Purpose

   Expresses human codon-optimized SpCas9-NRCH and blasticidin resistance: EFS promoter-SpCas9-NRCH-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-NRCH
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EFS

   Available since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGEX-parkin Ubl P37L

   Plasmid

   #227366

   Purpose

   For bacterial expression of mutated parkin Ubl.

   Depositor

   Kalle Gehring

   Article

   Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

   Insert

   Parkin 1-76 P37L (PRKN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Chan…

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pGEX-parkin K161N

   Plasmid

   #227365

   Purpose

   For bacterial expression of parkin with a mutated pUbl/pUb binding site on RING0.

   Depositor

   Kalle Gehring

   Article

   Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

   Insert

   Parkin K161N (PRKN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Chan…

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGEX-parkin delta 101-109

   Plasmid

   #227364

   Purpose

   For bacterial expression of parkin with a mutated ACT.

   Depositor

   Kalle Gehring

   Article

   Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

   Insert

   Parkin delta 101-109 (PRKN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Resi…

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGEX-parkin D106A

   Plasmid

   #227362

   Purpose

   For bacterial expression of parkin with a mutated ACT.

   Depositor

   Kalle Gehring

   Article

   Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

   Insert

   Parkin D106A (PRKN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Chan…

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pGEX-parkin V105K

   Plasmid

   #227361

   Purpose

   For bacterial expression of parkin with a mutated ACT.

   Depositor

   Kalle Gehring

   Article

   Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

   Insert

   Parkin V105K (PRKN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Chan…

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGEX-parkin T103A

   Plasmid

   #227360

   Purpose

   For bacterial expression of parkin with a mutated ACT.

   Depositor

   Kalle Gehring

   Article

   Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

   Insert

   Parkin T103A (PRKN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Chan…

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pGEX-parkin L102K

   Plasmid

   #227359

   Purpose

   For bacterial expression of parkin with a mutated ACT.

   Depositor

   Kalle Gehring

   Article

   Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

   Insert

   Parkin L102K (PRKN Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Codon usage optimized for E.coli expression. Chan…

   Promoter

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX-parkin M458L

    Plasmid

    #227356

    Purpose

    For bacterial expression of hyperactive parkin mutant.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin M458L (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pGEX-parkin R256C

    Plasmid

    #227355

    Purpose

    For bacterial expression of hyperactive parkin mutant.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin R256C (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX-parkin R234Q

    Plasmid

    #227354

    Purpose

    For bacterial expression of hyperactive parkin mutant.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin R234Q (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGEX-parkin V224A

    Plasmid

    #227353

    Purpose

    For bacterial expression of hyperactive parkin mutant.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin V224A (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX-parkin P37L

    Plasmid

    #227352

    Purpose

    For bacterial expression of hyperactive parkin mutant.

    Depositor

    Kalle Gehring

    Article

    Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

    Insert

    Parkin P37L (PRKN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Codon usage optimized for E.coli expression. Chan…

    Promoter

    Available since

    Nov. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSF_ApyADO

    Plasmid

    #226248

    Purpose

    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD and the cytochrome P450 enzyme ApyO

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Inserts

    His6_ApyA
    ApyD
    ApyO
    

    Use

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSF_ApyAO

    Plasmid

    #226242

    Purpose

    Production of ApyA peptide modified by the cytochrome P450 enzyme ApyO

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Inserts

    His6_ApyA
    ApyO
    

    Use

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSF_ApyAD

    Plasmid

    #226241

    Purpose

    Production of ApyA peptide modified by the B12-rSAM enzyme ApyD

    Depositor

    Wilfred van der Donk

    Article

    Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

    Inserts

    His6_ApyA
    ApyD
    

    Use

    Tags

    Hexa-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTE5418_pMBP-GlcR

    Plasmid

    #221192

    Purpose

    Expression vector for 10x-MBP-GlcR (MGlcR). GlcR is a glycolate-responsive transcriptional repressor and the gene product of pden4400 from Paracoccus denitrificans, codon optimized for E. coli.

    Depositor

    Tobias Erb

    Article

    Barthel et al Synth Biol (Oxf). 2024 Sep 20;9(1):ysae0

    Insert

    glcR (pden4400 from Paracoccus denitrificans)
    

    Use

    Tags

    10x His tag with E. coli maltose binding protein …

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter and T7 promoter-lacO

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMXS-IRES-Blast coUCK2 D62A

    Plasmid

    #211530

    Purpose

    Expresses codon optimized catalytic mutant UCK2

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    UCK2 D62A (UCK2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    D62A (catalytic mutant)

    Promoter

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMXS-IRES-Blast CG1358

    Plasmid

    #218541

    Purpose

    Drosophila CG1358 cDNA (human codon optimized)

    Depositor

    Kivanc Birsoy

    Article

    Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

    Insert

    CG1358 (HisT Fly)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. NylB-SCY_pET29b

    Plasmid

    #215415

    Purpose

    Expression construct for NylB-SCY gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    NylB-SCY
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    R187S/F264C/D370Y

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. M-NylC_pET28a

    Plasmid

    #215409

    Purpose

    Expression construct for M-NylC gene, codon optimized for expression in E. coli

    Depositor

    Gregg Beckham

    Article

    Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

    Insert

    M-NylC
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET23a-H6-TEV-AncSZ

    Plasmid

    #214235

    Purpose

    Bacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylation

    Depositor

    Neel Shah

    Article

    Hobbs et al Protein Sci. 2022 Oct;31(10):e4411. doi:

    Insert

    Syk kinase (SYK Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Sequence gained through ancestral reconstruction …

    Promoter

    T7

    Available since

    Feb. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCOA-Leu-FvPPT1

    Plasmid

    #179923

    Purpose

    Expression vector for heterologous expression of a polyketide synthase in S. cerevisiae. Carries and empty insertion site and the Fusarium verticillioides 4´-phosphopantetheinyltransferase PPT1

    Depositor

    Jens Laurids Sørensen

    Article

    Bejenari et al J Microbiol Biol Educ. 2023 Nov 27;24(3)

    Insert

    FvPPT1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Codon optimized for expression in Saccharomyces c…

    Promoter

    Yarrowia lipolytica EXP1

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GB1-His-TEV-A7 (2-180)

    Plasmid

    #190066

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain.

    Depositor

    Lalit Deshmukh

    Article

    Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

    Insert

    Annexin A7 PRD (ANXA7 Human)
    

    Use

    Tags

    GB1-His-TEV

    Expression

    Bacterial

    Mutation

    PRD only, residues 2-180

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. GB1-His-TEV-A7-Strep (2-180)

    Plasmid

    #190067

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses Annexin A7 proline rich domain with strep.

    Depositor

    Lalit Deshmukh

    Article

    Yu et al Biochemistry. 2023 Oct 3. doi: 10.1021/a

    Insert

    Annexin A7 PRD (ANXA7 Human)
    

    Use

    Tags

    GB1-His-TEV and Strep

    Expression

    Bacterial

    Mutation

    PRD only, residues 2-180

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. 1BR9-AtWUSSTOP

    Plasmid

    #202053

    Purpose

    E. coli expression vector for N-ter GFP11 tagged Arabidopsis thaliana WUSCHEL

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    AtWUS E. Coli Codon Optimized (WUS Mustard Weed)
    

    Use

    Tags

    6xHis and GFP11

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. 1BR9-AtWUS

    Plasmid

    #193865

    Purpose

    E. coli expression vector for N-ter GFP11 and C-ter R9 tagged WUSCHEL protein from Arabidopsis thaliana.

    Depositor

    Markita Landry

    Article

    Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

    Insert

    AtWUS E. Coli Codon Optimized
    

    Use

    Tags

    6xHis, GFP11, and R9

    Expression

    Bacterial

    Mutation

    Promoter

    T7 Promoter

    Available since

    Aug. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. GB1-PRD(703-868, P801G)

    Plasmid

    #180029

    Purpose

    Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-ALIX(703-868, P801G). P801G mutation facilitates expression in E. coli.

    Depositor

    Lalit Deshmukh

    Article

    Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

    Insert

    GB1-His6-TEV-ALIX(703-868, P801G) (PDCD6IP Human)
    

    Use

    Tags

    GB1-His6-TEV

    Expression

    Bacterial

    Mutation

    P801G

    Promoter

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEPQD1CB0001

    Plasmid

    #185625

    Purpose

    MoClo Level 1, position 2 reverse, transcriptional unit for expression of plant codon optimized Cas9 from Streptococcus pyogenes driven by 35S promoter

    Depositor

    Nicola Patron

    Article

    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:

    Insert

    SpCas9
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pNB203

    Plasmid

    #169114

    Purpose

    TESEC expression vector for mEGFP-Alr fusion construct

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    mEGFP-alr (alr Mycobacterium tuberculosis H37Rv, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pBAD

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pNB204

    Plasmid

    #169115

    Purpose

    TESEC expression vector for 6xHis-Alr fusion constrcut

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    6xHis-alr (alr Mycobacterium tuberculosis H37Rv, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pBAD

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pNB205

    Plasmid

    #169116

    Purpose

    TESEC expression vector for Asd from M. tuberculosis

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    asd (asd Mycobacterium tuberculosis H37Rv)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pBAD

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pNB206

    Plasmid

    #169117

    Purpose

    TESEC expression vector for CysH from M. tuberculosis

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    cysH (cysH Mycobacterium tuberculosis H37Rv)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pBAD

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pNB207

    Plasmid

    #169118

    Purpose

    TESEC expression vector for DapB from M. tuberculosis

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    dapB (dapB Mycobacterium tuberculosis H37Rv)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pBAD

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pNB208

    Plasmid

    #169119

    Purpose

    TESEC expression vector for TrpD from M. tuberculosis

    Depositor

    Edwin Wintermute

    Article

    Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

    Insert

    trpD (trpD Mycobacterium tuberculosis H37Rv)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for E. coli

    Promoter

    pBAD

    Available since

    July 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTL464

    Plasmid

    #69881

    Purpose

    a dCirl transcriptional reporter allele that contains an optimized gal4.2::p65 cassette at the start codon of the genomic dCirl ORF

    Depositor

    Robert Kittel, Tobias Langenhan

    Article

    Scholz et al Cell Rep. 2015 May 12;11(6):866-74. doi:

    Insert

    CIRL (Cirl Fly)
    

    Use

    Phic31-integration vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pcDNA3.1-Flag-stm2585-gp130

    Plasmid

    #174393

    Purpose

    Chimera of codon-optimized stm2585 (sarA/steE) and human gp130 sequence.

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    sarA-IL6ST chimera
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    140-177 of sarA replaced with homologous IL6ST se…

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA3.1-Flag-stm2585_Y167F

    Plasmid

    #174392

    Purpose

    S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with STAT3-binding YxxQ motif mutated to FxxQ

    Depositor

    Dennis Ko

    Article

    Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

    Insert

    stm2585
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Tyr 167 changed to Phe

    Promoter

    Available since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pUPD2 LbCas12a (GB1439)

    Plasmid

    #160573

    Purpose

    Human codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).

    Depositor

    Diego Orzaez

    Article

    Bernabe-Orts et al Plant Biotechnol J. 2019 Apr 5. doi: 10.

    Insert

    LbCas12a
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    BsaI and BsmBI sites removed

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge