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pcDNA5_TO_FRT_myc_GLUT4_WT_APEX2

Plasmid

#250766

Purpose

Mammalian cell expression of GLUT4

Depositor

Braden Lobingier

Article

Dagunts et al Sci Adv. 2026 Mar 20;12(12):eadx8715. do

Insert

GLUT4(WT)

Tags

APEX2 and myc (in exofacial loop)

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2-shH1

Plasmid

#241994

Purpose

Sleeping Beauty vector for inducible expression of a chicken H1 targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H1

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2-shH3

Plasmid

#241995

Purpose

Sleeping Beauty vector for inducible expression of a chicken H3 targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H3

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2-shH2B

Plasmid

#241996

Purpose

Sleeping Beauty vector for inducible expression of a chicken H2B targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H2B

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2-shH2A

Plasmid

#241997

Purpose

Sleeping Beauty vector for inducible expression of a chicken H2A targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H2A

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-1-shH2B

Plasmid

#240417

Purpose

Sleeping Beauty vector for inducible expression of a chicken H2B targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H2B

Expression

Mammalian

Promoter

TRE

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2-shH4

Plasmid

#240431

Purpose

Sleeping Beauty vector for inducible expression of a chicken H4 targeting shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

H4

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pHREP-KD-2-shCtrl

Plasmid

#240580

Purpose

Sleeping Beauty vector for inducible expression of a unspecific control shRNA using a shortened mir30 sequence. Allows co-selection with Puromycin.

Depositor

Lienhard Schmitz

Article

Pfisterer et al PLoS One. 2026 Feb 10;21(2):e0342014. do

Insert

Scramble shRNA

Expression

Mammalian

Available Since

Feb. 4, 2026

Availability

Academic Institutions and Nonprofits only

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pESC-leu2D-LjCPR1-GW

Plasmid

#233735

Purpose

Expresses plant cytochrome P450 in yeast for enzyme assay. Contains CPR1 from Lotus japonicus

Depositor

Sangeeta Dhaubhadel

Article

Khatri et al PLoS One. 2023 May 15;18(5):e0285698. do

Type

Empty backbone

Expression

Yeast

Promoter

Gal 1 and Gal 10

Available Since

Sept. 29, 2025

Availability

Academic Institutions and Nonprofits only

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MPS1 Schizosaccharomyces pombe

Plasmid

#232147

Purpose

MPS1 from Schizosaccharomyces pombe codon optimized for S. cerevisiae in pUCIDT

Depositor

Mark Winey

Article

Fabritius et al PLoS One. 2024 Mar 26;19(3):e0301084. do

Insert

MPS1 (mph1 Fission Yeast)

Expression

Yeast

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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fabp10a:FLAG-SpiCee-mRFP1; cryaa:mCherry

Plasmid

#230062

Purpose

Expression of genetically encoded calcium chelator in zebrafish hepatocytes.

Depositor

Sumeet Pal Singh

Article

Pozo-Morales et al Hepatology. 2023 Mar 1;77(3):789-801. do

Insert

SpiCee

Use

Zebrafish expression

Tags

FLAG and mRFP1

Promoter

fabp10a

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pRDB_268

Plasmid

#231112

Purpose

SpG-Cas9

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

Cas9-SpG

Use

CRISPR and Lentiviral

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA566

Plasmid

#231115

Purpose

Fragmid fragment: (guide cassette) ABE activity-based selection cloning

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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AA568

Plasmid

#231117

Purpose

Fragmid fragment: (guide cassette) CBE activity-based selection cloning

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC1_pPromALB_enh5898_FireflyLuc

Plasmid

#220285

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 5898 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 5898

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC2_pPromALB_enh29774_FireflyLuc

Plasmid

#220286

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 29774 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 29774

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC3_pPromALB_enh50363_FireflyLuc

Plasmid

#220287

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 50363 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 50363

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTYC4_pPromALB_enh50358_FireflyLuc

Plasmid

#220288

Purpose

Firefly luciferase with mouse liver DNase hypersensitive site # 50358 cloned into KpnI/XhoI-digested pPromALB_FireflyLuc

Depositor

David Waxman

Article

Chang et al BMC Genomics. 2024 Dec 24;25(1):1240. do

Insert

mouse liver DNase hypersensitive site # 50358

Use

Luciferase

Promoter

minimal albumin promoter

Available Since

Jan. 2, 2025

Availability

Academic Institutions and Nonprofits only

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insulator_seq_UENU_donor

Plasmid

#221459

Purpose

Vector from which reporter gene 3'UTRs, an enhancer, and a neutral DNA spacer are cloned for assembling an insulator-seq reporter library.

Depositor

Maria Cristina Gambetta

Article

Tonelli et al STAR Protoc. 2024 Oct 24;5(4):103391. do

Insert

3'UTR-OpIE2 enhancer-neutral DNA spacer-3'UTR

Use

Synthetic Biology

Available Since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-a(+)-6xHis-miRFP(-5)-HO1

Plasmid

#219424

Purpose

Fluorescent protein to reconstruct the mixture that mimics the charge profile of E. coli lysate

Depositor

Allie Obermeyer

Article

Ahn et al JACS Au. 2024 Sep 17;4(10):3800-3812. do

Inserts

miRFP682
HO1

Tags

MGHHHHHGG

Expression

Bacterial

Mutation

Contains Ribosomal Binding Site for expression of…

Promoter

T7

Available Since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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