We narrowed to 2,278 results for: dcas9

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pCMV MN (eGFP)

Plasmid

#89932

Purpose

Expresses the MN fragment only of the sMTase system for targeted DNA methylation in mammalian cells. Contains a eGFP marker expressed off separate promoter.

Depositor

Carl Novina

Article

Xiong et al Sci Rep. 2017 Jul 27;7(1):6732. doi: 10.

Insert

MN

Tags

HA

Expression

Mammalian

Mutation

M.SssI residues 2-272 fragment

Promoter

CMV

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pVC-Ds-Pax3a_enh5-E1b:eGFP-Ds

Plasmid

#119059

Purpose

Fluorescent vector with zebrafish pax3a enhancer 5 (strong activity)

Depositor

Tatjana Sauka-Spengler

Article

Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

Insert

pax3a_enh5-E1b:eGFP

Expression

Bacterial

Promoter

E1b minimal promoter

Available Since

Feb. 11, 2019

Availability

Academic Institutions and Nonprofits only

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JH401: pMVP/Ad/RGD-PK-DEST

Plasmid

#121846

Purpose

pMVP destination vector, empty RGD/PK fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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HB901: pMVP (L3-L2) polyA

Plasmid

#121746

Purpose

pMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

Polyadenylation signal (polyA)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JEH127

Plasmid

#179299

Purpose

Expresses dCas9 fused to VPR

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

dCas9-VPR

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

CAG

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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IF701: pMVP (L5-L4) APEX2

Plasmid

#121725

Purpose

pMVP L5-L4 entry plasmid, contains APEX2 for 4-component MultiSite Gateway Pro assembly. Allows fusion of N-term APEX2 to gene of interest.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

APEX2 (open)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IG301: pMVP (L1-R5) CMV promoter

Plasmid

#121692

Purpose

pMVP L1-R5 entry plasmid, contains CMV promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

CMV promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IZ401: pMVP/Lenti/Neo-DEST

Plasmid

#121850

Purpose

pMVP destination vector, empty Lentivirus vector backbone w/ Neo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Lentiviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KK001: pMVP/SB/Hygro-DEST

Plasmid

#121867

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Hygro selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LentiU6-mNeurog2 gRNA_1-MS2-Puro

Plasmid

#192680

Purpose

Lentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Mouse Neurog2 activating gRNA #1 (Neurog2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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HE401: pMVP (L1-R5) shRNA

Plasmid

#121698

Purpose

pMVP L1-R5 entry plasmid, contains empty H1-driven shRNA expression cassette for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JZ901: pMVP (L5-L4) EF1a promoter

Plasmid

#121727

Purpose

pMVP L5-L4 entry plasmid, contains EF1a promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

EF1a promoter

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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IG001: pMVP (L1-L4) TBG promoter

Plasmid

#121689

Purpose

pMVP L1-L4 entry plasmid, contains TBG promoter for 3-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TBG promoter (Liver specific)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JT504: pMVP/SB/eGFP-DEST

Plasmid

#121861

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eGFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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JT601: pMVP/SB/mCherry-DEST

Plasmid

#121862

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ mCherry selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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LF601: pMVP (L1-R5) TBG promoter

Plasmid

#121695

Purpose

pMVP L1-R5 entry plasmid, contains TBG promoter for 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

TBG promoter (Liver specific)

Use

Synthetic Biology; Pmvp gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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BPK1160

Plasmid

#179298

Purpose

Expresses dCas9 fused to P65

Depositor

Keith Joung

Article

Tak et al Nat Methods. 2021 Sep;18(9):1075-1081. d

Insert

dCas9-p65

Expression

Mammalian

Mutation

D10A, H840A (catalytically inactive Cas9)

Promoter

CAG

Available Since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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KN302: pMVP/SB/eCFP-DEST

Plasmid

#121863

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ eCFP selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KK901: pMVP/SB/Zeo-DEST

Plasmid

#121868

Purpose

pMVP destination vector, empty Sleeping Beauty transposon vector backbone w/ Zeo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Sleeping beauty transposon, pmvp gateway destinat…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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KJ101: pMVP (R4-R3) osTir1

Plasmid

#121742

Purpose

pMVP R4-R3 entry plasmid, contains osTir1 for 3- or 4-component MultiSite Gateway Pro assembly

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Insert

osTir1

Use

Synthetic Biology; Pmvp gateway entry plasmid

Tags

9x myc epitope tag

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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