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Plasmid#67285PurposeCPD photolyase from Drosophila with Flag tag in pFastbac vector. Intermediate in insect cell expressionDepositorInsertCPD photolyase (phr Fly)
UseBacterial vector, insert not expressedTagsFlagExpressionMutationV275E*PromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZA31
Plasmid#158509PurposeIn planta gene expression of Golden gate compatible N. benthamiana ZAR1 L17E-4xMyc. Km plant selection marker.DepositorInsertZAR1-4xMyc
UseTags4xMycExpressionPlantMutationL17EPromoterAvailable sinceAug. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAC1748
Plasmid#127982PurposeAMA1 plasmid with Aspergillus optimized Cpf1, argB selection marker, and USER cassette (PacI/Nt.BbvCI)DepositorInsertCpf1
UseCRISPR; Fungal expressionTagsSV40 NLSExpressionBacterialMutationcodon optimized for Aspergillus nidulansPromoterAspergillus nidulans tef1 promoterAvailable sinceDec. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCLC-mEos2
Plasmid#38010DepositorInsertClathrin, light polypeptide (LCa) (Clta Mouse)
UseTagsmEos2ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceSept. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGAD424-hSyntenin2b
Plasmid#89427PurposeTwo-hybrid systemDepositorInsertSDCBP2 (SDCBP2 Human)
UseTagsGal4 ADExpressionYeastMutation*see belowPromoterADH1Available sinceJuly 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUPD+Red-F
Plasmid#170874PurposePhytobrick - Red-F Luciferase CDSDepositorInsertRed-F
UseLuciferase and Synthetic BiologyTagsExpressionMutationp.S286Y Red mutantPromoterAvailable sinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_Lyn11-STf
Plasmid#182008PurposeCMV overexpression of SNAP-tag fast at the inner leaflet of the plasma membrane of mammalian cell linesDepositorInsertLyn11-STf
UseTagsExpressionMammalianMutationFast mutation on SNAP-tag E30RPromoterCMVAvailable sinceNov. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pNB813
Plasmid#60161PurposeYeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.DepositorInsertyEVenus-PEST
UseTagsPEST degronExpressionYeastMutationPromoterSIC1prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNB330
Plasmid#60147PurposeDisintegrator plasmid encoding MET17 promoter driven NanoLuc (Promega) with nuclear localization signal (NLS)DepositorInsertNLuc
UseTagsNuclear localization signal (NLS)ExpressionYeastMutationPromoterMET17prAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.244
Plasmid#73756PurposeNabi2.244 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.244
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pZA13
Plasmid#158491PurposeEntry clone for Golden Gate assembly. Golden gate compatible N. benthamiana ZAR1 without STOP codonDepositorInsertZAR1
UseGolden gate entry vectorTagsExpressionMutationno STOP codonPromoterAvailable sinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCoofy62
Plasmid#122014PurposeMammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag optionsDepositorTypeEmpty backboneUseTagsHis6-Smt3Star and Multi3: depending on the LP2 pr…ExpressionMammalianMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNB655
Plasmid#60142PurposeFirefly luciferase (Promega) yeast codon-optimized yellow fluorescent protein (yEVenus) fusion driven by MET17 promoter.DepositorInsertFLuc-yEVenus
UseTagsExpressionYeastMutationA4VPromoterMET17prAvailable sinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
Nabi2.242
Plasmid#73754PurposeNabi2.242 is a FRET (Förster Resonance Energy Transfer)-based protein voltage sensor. This probe contains Clover and mRuby2 inserted at different locations in the Ciona voltage sensitive domain.DepositorInsertNabi2.242
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmEos2-Linker_PCNA-MutC
Plasmid#98272Purposefor low level expression of mEos2-PCNA in mammalian cellsDepositorInsertPCNA (PCNA Human)
UseTagsmEos2ExpressionMammalianMutationSilent mutated Sequence: GACGCCGTAGTTATATCTTGC (O…PromoterCMVAvailable sinceJuly 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNB690
Plasmid#60134PurposeYellow beetle luciferase with nuclear localization signal (NLS) and ClpXP degron tag (ssrA), driven by MET17 promoter.DepositorInsertYeLuc
UseTagsClpXP degron and Nuclear localization signal (NLS…ExpressionYeastMutationPromoterMET17prAvailable sinceOct. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRKVSV FRNK
Plasmid#50534DepositorInsertprotein tyrosine kinase, domain
UseTags2X VSVExpressionMammalianMutationtruncated (NT2186-3268)PromoterCMVAvailable sinceMarch 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSG5-hSyntenin2b
Plasmid#89433Purposemammalian expressio of human Syntenin2bDepositorInsertSDCBP2 (SDCBP2 Human)
UseTagsExpressionMammalianMutationPromoterSV40Available sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBT9-hSyntenin2a
Plasmid#89421PurposeTwo-hybrid systemDepositorInsertSDCBP2 (SDCBP2 Human)
UseTagsGAL4 DBDExpressionYeastMutationPromoterADH1Available sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUAST td tomato Rab35 CA
Plasmid#58383Purposeexpression of drosophila tdtomato-Rab35 constitutively active mutantDepositorInsertRab35 (Rab35 Fly)
UseTagstd tomatoExpressionInsectMutationQ67L (constitutive active)PromoterGAL4Available sinceJuly 21, 2014AvailabilityAcademic Institutions and Nonprofits only