Addgene

We narrowed to 3,870 results for: pes

Showing: 1561 - 1580 of 3870 results

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pRepCap6

Plasmid

#110770

Purpose

pRepCap6 contains both rep and cap genes from the AAV6 genome, but lacks the viral terminal repeats (Rutledge et al., 1998). It lacks adenovirus helper functions required for stock production.

Depositor

David Russell

Article

Rutledge et al J Virol. 1998 Jan;72(1):309-19.

Insert

AAV6 cap, AAV6 rep genes

Use

Tags

Expression

Mutation

Promoter

Available since

June 22, 2018

Availability

Academic Institutions and Nonprofits only

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pPM1003

Plasmid

#167550

Purpose

Encodes genes that confer expression of Vibrio cholerae O antigens of the Ogawa serotype in E. coli K-12

Depositor

Renato Morona

Article

Manning et al Infect Immun. 1986 Aug;53(2):272-7. doi:

Insert

Vibrio cholerae O antigens genes of the Ogawa serotype

Use

Cosmid

Tags

Expression

Mutation

Promoter

Available since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-dAPEX2

Plasmid

#117173

Purpose

Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

dAPEX2

Use

AAV

Tags

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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SigmaR1-mNeonGreen

Plasmid

#226566

Purpose

Encodes SigmaR1 protein labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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ss-SigmaR1DTM-mNeonGreen

Plasmid

#226575

Purpose

Encodes mutant SigmaR1 protein (transmembrane region deletion) labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pRFHUE-eGFP

Plasmid

#89469

Purpose

GFP labelling of filamentous fungi by Agrobacterium tumefaciens mediated transformation

Depositor

Luis González-Candelas

Article

Crespo-Sempere et al Int J Food Microbiol. 2011 Aug 2;148(2):

Insert

eGFP

Use

Binary plasmid for atmt of filamentous fungi

Tags

Expression

Mutation

Promoter

A. nidulans gpdA

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP_mNG2(11)_Clathrin light chain

Plasmid

#82608

Purpose

Expresses mNG2(11) tagged clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

Insert

mNG2(11)_Clathrin light chain

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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Lyric-mNeonGreen

Plasmid

#226562

Purpose

Encodes Lyric protein labeled with mNeonGreen

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

Lyric (Lysine-rich CEACAM1 coisolated) (MTDH Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pC0044-EF1a-RanCas13b-NES-mapk

Plasmid

#103855

Purpose

Expresses RanCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

Depositor

Feng Zhang

Article

Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

Insert

RanCas13b

Use

CRISPR and Lentiviral

Tags

3xHA and mapk NES

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pOPTN-EGFP

Plasmid

#27052

Depositor

Beatrice Yue

Article

Park et al Am J Pathol. 2006 Dec . 169(6):1976-89.

Insert

optineurin (OPTN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2011

Availability

Academic Institutions and Nonprofits only

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pMF-TetTight

Plasmid

#193288

Purpose

Lentiviral construct that expresses rtTA-p2a-tTS for non-leaky dox-inducible expression of a transgene

Depositor

Rudolf Jaenisch

Article

Friesen et al Sci Adv. 2022 Jun 17;8(24):eabn7298. doi

Insert

rtTA-P2A-tTS

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF‐1α

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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Lyric-mCherry

Plasmid

#226563

Purpose

Encodes Lyric protein labeled with mCherry

Depositor

Gia Voeltz

Article

Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

Insert

Lyric (Lysine-rich CEACAM1 coisolated) (MTDH Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 12, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-rev-ChR2-tdtomato

Plasmid

#18917

Purpose

Cre-dependent viral expression of ChR2-tdTomato for neuronal excitation

Depositor

Scott Sternson

Article

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Insert

Channelrhodopsin 2-tdtomato

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

tdtomato

Expression

Mutation

Promoter

CAG

Available since

Feb. 9, 2009

Availability

Academic Institutions and Nonprofits only

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pCW-SPI1

Plasmid

#162824

Purpose

Doxycycline-inducible overexpression of human SPI1

Depositor

Matthew Steinhauser

Article

Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

Insert

SPI1 (SPI1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Tet ON

Available since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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AAV-FLEX-rev-ChR2(H134R)-mCherry

Plasmid

#18916

Purpose

Cre-dependent viral expression of ChR2(H134R)-mCherry for neuronal excitation

Depositor

Scott Sternson

Article

Atasoy et al J Neurosci. 2008 Jul 9. 28(28):7025-30.

Insert

Channelrhodopsin 2-mCherry

Use

AAV and Cre/Lox; Adeno-associated virus

Tags

mCherry

Expression

Mutation

H134R

Promoter

CAG

Available since

Jan. 29, 2009

Availability

Academic Institutions and Nonprofits only

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p{CFD4-3xP3::DsRed}

Plasmid

#86864

Purpose

Expresses DsRed in eyes and provides a backbone for expressing two gRNAs. With attB integration site.

Depositor

David Stern

Article

Stern et al G3 (Bethesda). 2017 Apr 3;7(4):1339-1347

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pVITRO-HPV33 L1L2

Plasmid

#52493

Purpose

Expresses HPV33 L1 and L2 in mammalian cells

Depositor

Richard Roden

Article

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV33 L1
HPV33 L2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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PB_iASCL1_P2A_DLX2_T2A_mCherry_Puro

Plasmid

#168806

Purpose

PiggyBac transposon construct with doxycycline inducible ASCL1, DLX2 + mCherry expression

Depositor

Prashant Mali

Article

Dailamy et al Stem Cell Reports. 2021 Sep 8. pii: S221

Insert

ASCL1-P2A-DLX2-T2A-mCherry

Use

Synthetic Biology; Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 31, 2021

Availability

Academic Institutions and Nonprofits only

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pUE1-TSP-BFLF1

Plasmid

#162657

Purpose

Expresses BFLF1 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

Depositor

Britt Glaunsinger

Article

Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

Insert

BFLF1

Use

Tags

N-terminal 2xStrep-Tag II and HRV 3C site

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pVITRO-HPV51 L1L2

Plasmid

#52583

Purpose

Expresses HPV51 L1 and L2 in mammalian cells

Depositor

Richard Roden

Article

Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

Inserts

HPV51 L1
HPV51 L2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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