Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 4,195 results for: spl

Clear keywords
Showing: 1561 - 1580 of 4195 results

  8. pCAG-DN-RFX

    Plasmid
    #52296
    Purpose
    Dominant-negatively inhibits RFX (regulatory factor X) transcription factor. Made of mouse RFX1 DNA binding domain (400-527 aa) and C-terminal myc tag.
    Depositor
    Christopher A Walsh
    Publication
    Bae et al Science. 2014 Feb 14;343(6172):764-8. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Dominant negative RFX
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    CAG promoter
    Available since
    April 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    52296-plasmid-map-sequence-id-80942

    Enlarge

  9. SF3A1

    Plasmid
    #25608
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SF3A1 (SF3A1 Human)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Ubiquitin-like domain
    Available since
    Aug. 30, 2010
    Availability
    Industry, Academic Institutions, and Nonprofits

  10. pAGK62

    Plasmid
    #228122
    Purpose
    Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells
    Depositor
    Seth Shipman
    Publication
    Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103
    Insert
    Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
    Expression
    Mammalian
    Promoter
    CAG for RT, U6/H1 for ncRNA/gRNA
    Available since
    Dec. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    228122-plasmid-map-sequence-id-455403

    Enlarge

  14. pM355

    Plasmid
    #173175
    Purpose
    (Empty Backbone) Expresses gRNA-12xMBS from hU6 promoter
    Depositor
    Hajin Kim
    Publication
    Chaudhary et al Genome Res. 2020 Sep;30(9):1306-1316. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    gRNA-12xMBS backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    hU6
    Available since
    Sept. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    173175-plasmid-map-sequence-id-341548

    Enlarge

  15. pSLQ8518 PB-PGK-N term (1-406) dLbCpf1-EE12RR345L-pA

    Plasmid
    #140221
    Purpose
    Constitutive PGK driven expression of N terminal dCpf1 (Cas12a) 406-split half with leucine zipper
    Depositor
    Stanley Qi
    Publication
    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dLbCpf1 (Cas12a) N terminal half (406) with zipper
    Expression
    Mammalian
    Promoter
    PGK
    Available since
    April 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    140221-plasmid-map-sequence-id-291991

    Enlarge

  18. mCherry-hPMCA2wb

    Plasmid
    #47752
    Purpose
    mammalian expression of mCherry tagged hPMCA2, wb splice variant
    Depositor
    Emanuel Strehler
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PMCA2w/b (ATP2B2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    wb splice variant
    Promoter
    CMV
    Available since
    Nov. 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  19. mCherry-hPMCA2zb

    Plasmid
    #47750
    Purpose
    mammalian expression of mCherry tagged hPMCA2, zb splice variant
    Depositor
    Emanuel Strehler
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PMCA2z/b (ATP2B2 Human)
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    zb splice variant
    Promoter
    CMV
    Available since
    Nov. 23, 2018
    Availability
    Academic Institutions and Nonprofits only

  20. pU6_B2M_sgRNA(PP7)

    Plasmid
    #232438
    Purpose
    sgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloop
    Depositor
    Gil Westmeyer
    Publication
    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter
    Use
    CRISPR
    Promoter
    U6
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    232438-plasmid-map-sequence-id-462755

    Enlarge

Showing: 1561 - 1580 of 4195 results