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pFOSGE_COF2H10

Plasmid

#70931

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

CG8939 (CG8939 Fly)

Use

Gateway entry vector

Available Since

Nov. 18, 2015

Availability

Academic Institutions and Nonprofits only
pJK376

Plasmid

#70235

Purpose

Protein production from a synthetic gene encoding Treponema denticola ATCC 35405 PurE (UniProt Q73PV9_TREDE)

Depositor

T. J. Kappock

Article

Tranchimand et al Biochemistry. 2011 May 31;50(21):4623-37

Insert

phosphoribosylaminoimidazole carboxylase

Expression

Bacterial

Promoter

T7

Available Since

Nov. 17, 2015

Availability

Academic Institutions and Nonprofits only

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pcDXNSMG3

Plasmid

#49029

Purpose

FX cloning mammalian cell line expression vector with CMV promoter and N-terminal streptavidin binding peptide, Myc tag, EGFP and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), EGFP, MYC, a…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pcDXC3MS

Plasmid

#49030

Purpose

FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, Myc tag and streptavidin binding peptide

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Tags

3C protease site (PreScission site), MYC, and str…

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pKK233-hCALML3

Plasmid

#47599

Depositor

Emanuel Strehler

Article

Rhyner et al Biochemistry. 1992 Dec 29;31(51):12826-3

Insert

CALML3 (CALML3 Human)

Expression

Bacterial

Mutation

contains 538 bp fragment

Promoter

trc

Available Since

Aug. 27, 2013

Availability

Academic Institutions and Nonprofits only

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p177bp-LacOx50-Hyg

Plasmid

#210213

Purpose

Integrates 50xLacO repeats with a hygromycin drug selection marker into 177bp repeat region.

Depositor

Calvin Tiengwe

Article

Budzak et al Mol Biochem Parasitol. 2023 Dec;256:1115

Insert

50xLacO repeats derived from: PMID: 12864855.

Available Since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003-mi3

Plasmid

#159995

Purpose

Expresses SpyCatcher003-mi3 in bacterial cells as virus-like particle platform for plug-and-display vaccination

Depositor

Mark Howarth

Article

Rahikainen et al Angew Chem Int Ed Engl. 2020 Sep 4. doi:

Insert

SpyCatcher003-mi3

Tags

C-Tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 9, 2020

Availability

Academic Institutions and Nonprofits only

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mTurquoise2

Plasmid

#198196

Purpose

Spectral correction: Expression of mTurquoise2

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mTurquoise2

Expression

Mammalian

Mutation

A206K

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEZT-BM

Plasmid

#74099

Purpose

Multi-purpose rapid titer bacmam expression vector

Depositor

Ryan Hibbs

Article

Morales-Perez et al Structure. 2016 Mar 24. pii: S0969-2126(

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Bacmam, baculovirus

Tags

EGFP following p10 promoter

Expression

Mammalian

Promoter

CMV, p10

Available Since

March 28, 2016

Availability

Academic Institutions and Nonprofits only

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p15aC-4D1D

Plasmid

#107864

Purpose

Bacterial expression of PIS and PI4K

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-kinase β

Tags

myc

Expression

Bacterial

Promoter

proD

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pYPQ230-sbcB-N

Plasmid

#220290

Purpose

Gateway entry plasmid (attL1& attR5) expressing sbcB exonuclease fused to the N-terminus of LbCas12a, connected by a flexible XTEN linker, without promoter

Depositor

Yiping Qi

Article

Wang et al Plant Physiol. 2025 Oct 31;199(3):kiaf50

Insert

sbcB-XTEN linker-LbCas12a

Use

CRISPR; Gateway compatible sbcb-xten linker- lbca…

Expression

Plant

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pNL3.2.CMV.NLuc-PEST.Hygro

Plasmid

#209998

Purpose

In mammalian cells expresses NanoLuc mRNA with no TH as a control. Also expresses a hygromycin resistance marker.

Depositor

Kevin Weeks

Article

Hatfield et al Biochemistry. 2025 Sep 16;64(18):3854-38

Inserts

NanoLuc
Hygromycin resistance marker

Expression

Mammalian

Promoter

CMV and SV40

Available Since

July 2, 2025

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4a

Plasmid

#83166

Purpose

Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions)

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Available Since

Sept. 14, 2016

Availability

Academic Institutions and Nonprofits only

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mVenus

Plasmid

#198192

Purpose

Spectral correction: Expression of mVenus

Depositor

Christian Wahl-Schott

Article

Feldmann et al STAR Protoc. 2023 Jul 28;4(3):102459. do

Insert

mVenus

Expression

Mammalian

Mutation

A206K

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-CA

Plasmid

#105665

Purpose

expresses thermostable variant of human carbonic anhydrase II with C-terminal 6x his tag

Depositor

Ryan Mehl

Article

Mehl et al 2020

Insert

human carbonic anhydrase II thermostable variant (CA2 Human)

Tags

6x his tag

Expression

Bacterial

Promoter

pBad/arabinose

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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peYFP-C1-mTOR

Plasmid

#73384

Purpose

Expresses YFP tagged mTOR

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

mTOR (MTOR Human)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR4-GFP-C1 (w392-1)

Plasmid

#17396

Purpose

Gateway entry vector for N-term eGFP fusion, frame C1

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

GFP

Use

Entry vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pMSCVpuro-TagRFP657

Plasmid

#96939

Purpose

Retroviral plasmid containing TagRFP657 for labeling of cells

Depositor

Lee Grimes

Article

Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

Insert

Tag Red Fluorescent Protein 657

Expression

Mammalian

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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pBad-sfGFP

Plasmid

#85482

Purpose

Arabinose inducible E. coli codon optimized superfolder GFP with C-terminal His6 tag

Depositor

Ryan Mehl

Article

Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

Insert

superfolder GFP

Tags

6x His

Expression

Bacterial

Promoter

Arabinose

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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Hs.KRAS4b G12C

Plasmid

#83130

Purpose

Gateway ORF Entry clone of human KRAS4B [NM_004985.4 ] with stop codon (for native or N-terminal fusions), G12C mutation

Depositor

Dominic Esposito

Insert

KRAS (KRAS Human)

Use

Gateway entry clone

Mutation

G12C

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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