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Showing: 7901 - 7910 of 7994 results

  7. pLV-dCas9-p300-P2A-PuroR

    Plasmid
    #83889
    Purpose
    Lentiviral Sp dCas9-p300-P2A-PuroR
    Depositor
    Charles Gersbach
    Article
    Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038
    Inserts
    S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 Human, S. pyogenes, Synthetic)
    pac from Streptomyces alboniger
    Use
    CRISPR and Lentiviral
    Tags
    Flag and nucleoplasmin NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    EFS
    Available Since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    83889-plasmid-map-sequence-id-297764

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  8. MB 94C thsS(t3)R-Bxb1_P7-sfGFP_mCherry

    Plasmid
    #232471
    Purpose
    Optimized thiosulfate sensor with recombinase switch and fluorescent reporter (GFP), constitutive mCherry
    Depositor
    Mikhail Shapiro
    Article
    Buss et al Nat Commun. 2025 Aug 25;16(1):7931. doi:
    Inserts
    thsS(t3)
    thsR
    PphsA342
    sfGFP
    AxeTxe
    mCherry
    Bxb1 integrase
    Tags
    ssrA degradation tag
    Expression
    Bacterial
    Mutation
    Contains the following mutations K286Q, Q350H, I…
    Promoter
    J23100 (TTGACGGCTAGCTCAGTCCTAGGTACAGTGCTAGC), J23…
    Available Since
    Sept. 17, 2025
    Availability
    Academic Institutions and Nonprofits only
    232471-plasmid-map-sequence-id-472144

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  10. pcDNA3.1-NLS-myc-dL5-2xG4S-mCer3

    Plasmid
    #73205
    Purpose
    Expresses myc-dL5(E52D)-mCer3 fusion protein in nuclei of mammalian cells. (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    NLS-myc-dL5-2XG4S-mCer3 (MYC Human, Synthetic)
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available Since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73205-plasmid-map-sequence-id-139718

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Showing: 7901 - 7910 of 7994 results