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pDESTtol2pACrymCherry

Plasmid

#64023

Purpose

Gateway destination vector with zebrafish aA-crystallin promoter driving mCherry GFP in lens

Depositor

Peter Currie, Joachim Berger

Article

Berger et al Genesis. 2013 Jun;51(6):443-7. doi: 10.1

Insert

mCherry

Use

Gateway

Tags

Expression

Mutation

Promoter

alpha-Acrystallin promoter

Available since

May 18, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2(+)-zGrad

Plasmid

#119716

Purpose

Plasmid to generate zGrad mRNA to inactivate proteins in zebrafish

Depositor

Holger Knaut

Article

Yamaguchi et al Elife. 2019 Feb 8;8. pii: 43125. doi: 10

Insert

zGrad

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-gRNA

Plasmid

#46759

Purpose

gRNA cloning vector for in vitro transcription of target gRNA

Depositor

Wenbiao Chen

Article

Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

Insert

gRNA core

Use

CRISPR; Zebrafish expression

Tags

Expression

Mutation

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only

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slc1a3b:mCD8mCherry

Plasmid

#170207

Purpose

Drives mCD8mCherry expression with the slc1a3b promoter in astrocytes

Depositor

Kelly Monk

Article

Chen et al Nat Neurosci. 2020 Oct;23(10):1297-1306.

Insert

mCD8

Use

Zebrafish

Tags

mCherry

Expression

Mutation

Promoter

slc1a3b

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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pT1UciMP

Plasmid

#62215

Purpose

UAS transgenesis vector

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Type

Empty backbone

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

UAS-E1b

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pT1QciMP

Plasmid

#62216

Purpose

QUAS transgenesis vector

Depositor

Harold Burgess

Article

Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

Type

Empty backbone

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

QUAS-E1b

Available since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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p5E-dusp6

Plasmid

#82584

Purpose

5' entry vector containing FGF-responsive regulatory element of the zebrafish dusp6 promoter for FGF-responding cells

Depositor

Kryn Stankunas

Article

Akerberg et al PLoS One. 2014 Mar 18;9(3):e92217. doi:

Insert

FGF-responsive element of the dusp6 promoter

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pCS2-hcar1-4-mKate2

Plasmid

#226712

Purpose

overexpression red fluorescent hcar1-4 protein in zebrafish larvae

Depositor

Philipp Niethammer

Article

Ma et al Cell Rep. 2023 Jan 31;42(1):111974. doi:

Insert

hcar1-4

Use

Unspecified; Zebrafish expression

Tags

mKate2

Expression

Mutation

Promoter

SP6

Available since

Nov. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pT3TS-spRYCas9-ABE-UMax

Plasmid

#222139

Purpose

Near PAMless Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

TadA-nSpRYCas9

Use

CRISPR; Zebrafish expression

Tags

bipartite NLS

Expression

Mutation

Promoter

T3

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Cas9-T2A-GFP

Plasmid

#63155

Purpose

Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

Depositor

Leonard Zon

Article

Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

Insert

Cas9-T2A-GFP

Use

CRISPR; Tol2 middle entry vector for gateway

Tags

Expression

Mutation

Promoter

Available since

April 1, 2015

Availability

Academic Institutions and Nonprofits only

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th-P2A-EGFP donor

Plasmid

#65562

Purpose

Intron targeting-mediated EGFP knockin at the zebrafish th locus.

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

th-P2A-EGFP (th Zebrafish)

Use

Zebrafish injection

Tags

Expression

Mutation

Promoter

none

Available since

June 3, 2015

Availability

Academic Institutions and Nonprofits only

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pT3TS-spCas9-ABE-Umax-rest1

Plasmid

#222144

Purpose

Adenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Article

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Expression

Mutation

Promoter

T3

Available since

July 8, 2024

Availability

Academic Institutions and Nonprofits only

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th-P2A-Gal4 donor

Plasmid

#65563

Purpose

Intron targeting-mediated Gal4 knockin at the zebrafish th locus.

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

th-P2A-Gal4 (th Zebrafish)

Use

Zebrafish injection

Tags

Expression

Mutation

Promoter

none

Available since

June 4, 2015

Availability

Academic Institutions and Nonprofits only

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acta1-Cavin1a-Clover

Plasmid

#163852

Purpose

Zebrafish muscle specific expression of Cavin1a-Clover, Parton lab clone DPR

Depositor

Rob Parton

Article

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Cavin1a (cavin1a Zebrafish)

Use

Zebrafish expression

Tags

Clover

Expression

Mutation

Promoter

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pTol1-UAS:ChR2(H134R)-tdTomato

Plasmid

#124237

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChR2(H134R) fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

ChR2(H134R)-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Expression

Mutation

Promoter

14xUAS-E1b

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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MiniCoopR mitfa:SPRED1

Plasmid

#118851

Purpose

Expresses human SPRED1 and zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Article

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

SPRED1 (SPRED1 Human, Zebrafish)

Use

Zebrafish expression, tol7

Tags

Expression

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pminiTol2-4kb wnt3 promoter-Fzd1mApple

Plasmid

#203795

Purpose

Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Fzd1-mApple for zebrafish transgenesis

Depositor

Thorsten Wohland

Article

Veerapathiran et al Elife. 2020 Nov 25;9:e59489. doi: 10.755

Insert

Fzd1 (fzd1 Zebrafish)

Use

Zebrafish expression

Tags

mApple

Expression

Mutation

Promoter

4kb Wnt3 promoter

Available since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221-zgc:152904

Plasmid

#192740

Purpose

Gateway entry vector encoding zebrafish zgc:152904

Depositor

Summer Thyme

Insert

zgc:152904 (zgc:152904 Zebrafish)

Use

Gateway entry vector

Tags

Expression

Mutation

N56D; 663T_664DinsA; 733V_772Edel (exon 24)

Promoter

None

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-usp25

Plasmid

#192733

Purpose

Gateway entry vector encoding zebrafish usp25

Depositor

Summer Thyme

Insert

usp25 (usp25 Zebrafish)

Use

Gateway entry vector

Tags

Expression

Mutation

S403R; W622R; 725A_726AinsAA (rs508161559); A730T…

Promoter

None

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR221-psmg1

Plasmid

#192723

Purpose

Gateway entry vector encoding zebrafish psmg1

Depositor

Summer Thyme

Insert

psmg1 (psmg1 Zebrafish)

Use

Gateway entry vector

Tags

Expression

Mutation

R96Q (rs514814252); T152M (rs501951686); T189A (r…

Promoter

None

Available since

Nov. 14, 2022

Availability

Academic Institutions and Nonprofits only

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