Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 211 - 240 of 22804 results

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Flag-TRIM24-F869D/F979A/N980A shRescue

Plasmid

#28145

Depositor

Michelle Barton

Insert

TRIM24 (TRIM24 Human)

Tags

FLAGX2

Expression

Mammalian

Mutation

F869D, F979A, N980A and aa 308 and 309 mutated fo…

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
KL060 - pBlueScript II SK-mAID-EGFP CTCF donor

Plasmid

#232926

Purpose

Donor plasmid for tagging mouse CTCF with mAID-eGFP at the N-terminus. Used with KL061 - PX330-sgRNA006_CTCF sgRNA.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

mAID-eGFP with mCTCF homology arms

Use

Mouse Targeting

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pC0096 RESCUE truncation (Cas13 delta 892-1095)

Plasmid

#130663

Purpose

CMV-dCas13b6(delta 892-1095)-mapkNES-GS-RESCUE

Depositor

Feng Zhang

Article

Abudayyeh et al Science. 2019 Jul 11. pii: science.aax70

Insert

dRanCas13b(delta 892-1095)-ADAR2dd

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET His6 prescission LIC cloning vector (HPKS)

Plasmid

#29720

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis and Prescission cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pzk384-pmax-PUFc-FseI-RESCUE-SalI-NES

Plasmid

#196831

Purpose

Express PUFc-RESCUE-S

Depositor

Albert Cheng

Article

Liu et al Nucleic Acids Res. 2023 Aug 11;51(14):e7

Insert

PUFc-RESCUE

Expression

Mammalian

Available Since

March 29, 2024

Availability

Academic Institutions and Nonprofits only

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KL158 - pBlueScript II SK-T2A-eGFP-SV40pA Podxl donor

Plasmid

#232936

Purpose

Donor plasmid for knock-in of T2A-eGFP-SV40pA at the mouse Podxl locus. Used with PX459-sgRNA047_Podxl sgRNA.

Depositor

Danny Reinberg

Article

Lucero et al bioRxiv, March 19, 2025

Insert

T2A-eGFP-SV40pA with Podxl homology arms

Use

Mouse Targeting

Available Since

March 26, 2025

Availability

Academic Institutions and Nonprofits only

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pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop cKit HDR

Plasmid

#207135

Purpose

DNA sequence source for amplifying an HDR template for mouse cKit knockout and reporter knock-in

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

5'HDR cKit- ZsGreen- sv40(polyA) - 3'HDR cKit (Kit Mouse)

Expression

Mammalian

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBluescript II Ks(+)-ZsGreen1-SV40 PolyA-Stop Mettl3 HDR

Plasmid

#207136

Purpose

DNA sequence source for amplifying an HDR template for mouse Mettl3 knockout and reporter knock-in

Depositor

Silvia Monticelli

Article

Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

Insert

5'HDR Mettl3 - ZsGreen- sv40(polyA) - 3'HDR Mettl3 (Mettl3 Mouse)

Expression

Mammalian

Available Since

Dec. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET His6 MBP prescission LIC cloning vector (HMPKS)

Plasmid

#29721

Depositor

Scott Gradia

Type

Empty backbone

Tags

6xHis, MBP, and Prescission cleavage site

Expression

Bacterial

Promoter

T7-lacO (lactose/IPTG inducible)

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-IRES-puro-NLS-dEsCas13d-EGFP-NLS-3xFlag

Plasmid

#132410

Purpose

overexpression in human cells

Depositor

Ling-Ling Chen

Article

Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

Insert

dEsCas13b

Use

Lentiviral

Tags

flag

Expression

Mammalian

Mutation

R295A; H300A; R849A; H854A

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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CMV-minidCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

Plasmid

#171385

Purpose

Expression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Article

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized minidCas13X.1-RESCUE-S

Expression

Mammalian

Promoter

CMV, U6

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-dCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

Plasmid

#171384

Purpose

Expression vector for encoding a human codon-optimized dCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Article

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized dCas13X.1-RESCUE-S

Expression

Mammalian

Promoter

CMV, U6

Available Since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

Plasmid

#55217

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

July 9, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

Plasmid

#55216

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V1)

Plasmid

#55222

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

June 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick PolyPromoter LIC Subcloning (438-SNAP-V3)

Plasmid

#55223

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available Since

July 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
KHBD00383

Plasmid

#34496

Depositor

Bart Deplancke

Article

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG14941 (esc Fly)

Use

Gateway donor vector

Available Since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pFloxin

Plasmid

#24553

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting

Available Since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pFloxin-MCS2-IRES-MCS

Plasmid

#24642

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting

Available Since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pFloxin-IRES-eGFP

Plasmid

#24557

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Insert

eGFP

Use

Cre/Lox and Mouse Targeting

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pFloxin-TAP

Plasmid

#24555

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting

Tags

TAP

Available Since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pFloxin-IRES

Plasmid

#24554

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pFloxin-IRES-TAP

Plasmid

#24556

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Type

Empty backbone

Use

Cre/Lox and Mouse Targeting

Tags

TAP

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge
pIRES2-eGFP-Ofd1-Myc

Plasmid

#24560

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Insert

Ofd1 (Ofd1 Mouse)

Tags

Myc and eGFP

Expression

Mammalian

Available Since

May 20, 2010

Availability

Academic Institutions and Nonprofits only

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pFloxin-IRES-Ofd1-Myc

Plasmid

#24559

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Available Since

May 27, 2010

Availability

Academic Institutions and Nonprofits only

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pFloxin-Ofd1-Myc

Plasmid

#24558

Depositor

Jeremy Reiter

Article

Singla et al Nat Methods. 2010 Jan . 7(1):50-2.

Insert

Ofd1 (Ofd1 Mouse)

Use

Cre/Lox and Mouse Targeting

Tags

Myc

Mutation

includes exons 4-23 only

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only
pFOSGE_COF1E11

Plasmid

#70850

Purpose

Gateway entry clone

Depositor

Alexander Stark

Article

Stampfel et al Nature. 2015 Nov 9. doi: 10.1038/nature1

Insert

escl (escl Fly)

Use

Gateway entry vector

Available Since

Dec. 7, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3-GFP-Dub3 mouse

Plasmid

#72683

Purpose

mammalian expression of GFP-Dub3

Depositor

Domenico Maiorano

Article

van_der_Laan et al Mol Cell. 2013 Nov 7;52(3):366-79. doi:

Insert

Dub3 (Usp17le Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Dub3 mouse

Plasmid

#72682

Purpose

mammalian expression of mouse Dub3

Depositor

Domenico Maiorano

Article

van_der_Laan et al Mol Cell. 2013 Nov 7;52(3):366-79. doi:

Insert

Dub3 (Usp17le Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only
pPB-tetpA-iRPT

Plasmid

#140763

Purpose

Expresses iRFP713, PuroR and rtTAM2 in mammalian cells, with a TRE cassette

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Neurosci Res. 2019 Oct 3. pii: S0168-010

Inserts

iRFP713
PuroR
rtTAM2

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge

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