Skip to main content
Addgene
Showing: 211 - 240 of 943 results
  1. pTJK477

    Plasmid
    #138005
    Purpose
    3xMYC-SIX2 expression. Vector also expresses GFP under control of hUBC promoter
    Depositor
    Insert
    3xMYC tagged SIX2 (SIX2 Human)
    Use
    Lentiviral
    Tags
    3xMYC
    Expression
    Mutation
    None
    Promoter
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
  2. pTJK421

    Plasmid
    #138007
    Purpose
    BirA*-SIX2 expression. Vector also expresses GFP under control of hUBC promoter
    Depositor
    Insert
    BirA* tagged SIX2 (SIX2 Human)
    Use
    Lentiviral
    Tags
    BirA*
    Expression
    Mutation
    None
    Promoter
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
  3. mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177435
    Purpose
    To express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.
    Depositor
    Insert
    Phosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  4. pLenti-L3US2-RFP-HDAC3

    Plasmid
    #83966
    Purpose
    Lentiviral CRISPR HDAC3 dual gRNA targeting vector
    Depositor
    Insert
    HDAC3 dual gRNAs (HDAC3 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
  5. pSLIK DNM1L-V5 neo

    Plasmid
    #220363
    Purpose
    Lentiviral expression vector for an inducible V5-tagged DNM1L construct
    Depositor
    Insert
    DNM1L (DNM1L Human)
    Use
    Lentiviral
    Tags
    V5
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 23, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. pLgw ELK1Y158A-V5-EcoDam

    Plasmid
    #98609
    Purpose
    Mammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloning
    Depositor
    Insert
    ELK1Y158A (Elk1 Mouse)
    Use
    Lentiviral; Damid
    Tags
    V5 and Dam (DNA adenine methyltransferase)
    Expression
    Mammalian
    Mutation
    Promoter
    Heat Shock Minimal Promoter
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
  7. pLgw EcoDam-V5-ELK1Y158A

    Plasmid
    #98608
    Purpose
    Mammalian DamID lentiviral vector for fusing ELK1Y158A with Dam-V5 using Gateway cloning
    Depositor
    Insert
    ELK1Y158A (Elk1 Mouse)
    Use
    Lentiviral; Damid
    Tags
    Dam (DNA adenine methyltransferase) and V5
    Expression
    Mammalian
    Mutation
    Promoter
    Heat Shock Minimal Promoter
    Available since
    July 28, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pCAGGS-FuG-B

    Plasmid
    #67523
    Purpose
    This is an envelope gene encoding plasmid to make retrogradely lentiviral vector. You can make type B envelope coating virus particle with HiRet by using this plasmid.
    Depositor
    Insert
    Fusion Glycoprotein type B
    Use
    Lentiviral
    Tags
    Fusion protein of Vesicular stomatitis Indiana vi…
    Expression
    Mutation
    Promoter
    CAGGS
    Available since
    July 31, 2015
    Availability
    Academic Institutions and Nonprofits only
  9. lenti.Cas9.BFP.sgRNA.parental

    Plasmid
    #196715
    Purpose
    Sortable WT-SpCas9 expression and guide cassette (1-vector system).
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Nucleoplasmin NLS and SV40 NLS
    Expression
    Mutation
    Promoter
    Available since
    April 12, 2023
    Availability
    Academic Institutions and Nonprofits only
  10. pTJK438

    Plasmid
    #138004
    Purpose
    3xMYC-SIX1 expression. Vector also expresses GFP under control of hUBC promoter
    Depositor
    Insert
    3xMYC tagged SIX1 (SIX1 Human)
    Use
    Lentiviral
    Tags
    3xMYC
    Expression
    Mutation
    None
    Promoter
    Available since
    May 18, 2020
    Availability
    Academic Institutions and Nonprofits only
  11. pLKO.1 puro-humanU6-shRNA FASN

    Plasmid
    #82327
    Purpose
    used to silence target human FASN expression via RNA interference (3rd gen lentiviral vector)
    Depositor
    Insert
    shRNA to target Fatty Acid Synthase (FASN) (FASN Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Sept. 30, 2016
    Availability
    Academic Institutions and Nonprofits only
  12. pCD/NL-BH∆1

    Plasmid
    #41791
    Purpose
    Packaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding Rev
    Depositor
    Insert
    HIV-1 Gag/Pol and RRE sequences
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
  13. pBEG R2-L3 #BGV193

    Plasmid
    #48952
    Purpose
    For inserting selection gene between AttR2 and AttL3 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)
    Depositor
    Type
    Empty backbone
    Use
    Gateway cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  14. pBEG R3-L4 #BGV194

    Plasmid
    #48954
    Purpose
    For inserting selection gene between AttR3 and AttL4 sites for use in Gateway recombination cloning system (corresponds to a module 3 plasmid)
    Depositor
    Type
    Empty backbone
    Use
    Gateway cloning vector
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
  15. LentiCRISPRv2-mCherry

    Plasmid
    #99154
    Purpose
    Lentiviral vector encoding sgRNA cloning site + hSpCAS9-P2A-mCherry.
    Depositor
    Inserts
    Cas9
    mCherry
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    Aug. 10, 2017
    Availability
    Academic Institutions and Nonprofits only
  16. pLV-EBFP2-nuc

    Plasmid
    #36085
    Depositor
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Replace EYFP of the vector pEYFP-Nuc from Clontec…
    Promoter
    Available since
    June 1, 2012
    Availability
    Academic Institutions and Nonprofits only
  17. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid
    #106477
    Purpose
    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens
    Depositor
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
  18. pUltra-H2B-mTagBFP2

    Plasmid
    #161791
    Purpose
    3rd generation lentiviral multicistronic vector for mammalian expression of EGFP, H2B-mTagBFP2, and one gene of interest
    Insert
    H2B-mTagBFP2
    Use
    Lentiviral; Multicistronic
    Tags
    EGFP and mTagBFP2
    Expression
    Mammalian
    Mutation
    I174A in the mTagBFP2 protein according to PLoS O…
    Promoter
    UbC
    Available since
    Nov. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
  19. psd44-GqQL

    Plasmid
    #46826
    Purpose
    2nd generation lentiviral vector for expression of mutant G protein alpha q in mammalian cells
    Depositor
    Insert
    g protein alpha q (GNAQ Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Q209L (The mutation reduces GTPase activity resul…
    Promoter
    PGK promoter
    Available since
    July 30, 2013
    Availability
    Academic Institutions and Nonprofits only
  20. mCherry-Cb5-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177434
    Purpose
    To express mCherry fused to Cb5 endoplasmic reticulum (ER) targeting sequence. Lentiviral vector used to make cell lines expressing this ER landmark.
    Depositor
    Insert
    Cb5 tail anchor (Cyb5r4 Rat)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
  21. pLV-SFFV-CALU-WPRE-UbC-Emerald

    Plasmid
    #225951
    Purpose
    Lentiviral vector plasmid expressing human calumenin (CALU) under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter
    Inserts
    CALU (CALU Human)
    Emerald
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    SFFV and UbC
    Available since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
Showing: 211 - 240 of 943 results