Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 442 results for: SHB

Showing: 281 - 320 of 442 results

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pcDNA5FRT/Flag-hINO80N1 (1-262)

Plasmid

#29441

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 1-262 (a.a.)

Available Since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only
pEGFPc1 QFR IP3R3

Plasmid

#236454

Purpose

transient overexpression of IP3R3 in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nature. 2017 Jun 22;546(7659):554-558. d

Insert

IP3R3 (Itpr3 Rat)

Expression

Mammalian

Mutation

Q-FR/A-AA

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Step-FLAG FBXO21

Plasmid

#236458

Purpose

transient overexpression of FBXO21 in mammalian cells

Depositor

Michele Pagano

Article

Donato et al Nat Cell Biol. 2017 Apr;19(4):341-351. d

Insert

FBXO21 (FBXO21 Human)

Tags

Strep-FLAG

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT to BioID-nDIC2-3xFlag

Plasmid

#232418

Purpose

Expresses human dynein subunit DIC2 in mammalian cells.

Depositor

Samara Reck-Peterson

Article

Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

Insert

Dynein Intermediate Chain 2 (DYNC1I2 Human)

Tags

BioID and Flag-3x

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5 FRT to BioID-cDIC2-3xFlag

Plasmid

#232416

Purpose

Expresses human dynein subunit DIC2 in mammalian cells.

Depositor

Samara Reck-Peterson

Article

Redwine et al Elife. 2017 Jul 18;6:e28257. doi: 10.755

Insert

Dynein Intermediate Chain 2 (DYNC1I2 Human)

Tags

BioID and Flag-3x

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-GAL4-PARP1-CAT-L713F

Plasmid

#175949

Purpose

Bacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

GAL4 DNA binding domain (GAL4 Budding Yeast)

Tags

6xHis tag

Expression

Bacterial

Mutation

L713F, V762A for the 'PARP1-CAT-L713F' …

Promoter

T7

Available Since

July 19, 2024

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-A762V

Plasmid

#174795

Purpose

Bacterial expression of wild type PARP1 mutant (A762V is reversion of the common V762A polymorphism)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A762V (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

A762V

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas9-mD3A

Plasmid

#78257

Purpose

Expresses dead Cas9 (dCas9) fused to inactive DNMT3A catalytic domain (CD) under the control of the CMV promoter

Depositor

Grant Challen

Article

McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

Insert

DNMT3A CD aa 598-912 (DNMT3A Human)

Use

CRISPR

Tags

Flag epitope tag

Expression

Mammalian

Mutation

E756A

Promoter

pCMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas9-D3A

Plasmid

#78256

Purpose

Expresses dead Cas9 (dCas9) fused to DNMT3A catalytic domain (CD) under the control of the CMV promoter

Depositor

Grant Challen

Article

McDonald et al Biol Open. 2016 May 11. pii: bio.019067.

Insert

DNMT3A CD aa 598-912 (DNMT3A Human)

Use

CRISPR

Tags

Flag epitope tag

Expression

Mammalian

Promoter

pCMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only

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pOGS539_haPD-1

Plasmid

#226716

Purpose

Plasmid enabling yeast-mediated expression and secretion of high affinity PD-1 microbody (haPD-1)

Depositor

Gautam Dantas

Article

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

High affinity PD-1 microbody

Tags

HA tag and Mating factor alpha secretion signal

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-Flag-Med25

Plasmid

#64771

Purpose

Mammalian protein expression of flag-tagged Med25

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

Med25 (MED25 Human)

Tags

Flag

Expression

Mammalian

Promoter

pCMV

Available Since

May 19, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5-F-Med26-NTD

Plasmid

#49255

Purpose

Mammalian expression of flag-tagged Med26-NTD

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med 26 (MED26 Human)

Tags

Flag

Expression

Mammalian

Mutation

NTD (aa 1-339)

Available Since

Dec. 30, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5-F-Med26-R61A K62A

Plasmid

#49254

Purpose

Mammalian expression of flag-tagged human Med26 R61A K62A

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

Med26 (MED26 Human)

Tags

Flag

Expression

Mammalian

Mutation

R61A K62A

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1-E988K

Plasmid

#173945

Purpose

Bacterial expression of inactive PARP1 mutant (E988K attenuates catalytic and PAR-branching activity of PARP1)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-E988K (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

V762A, E988K

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1deltaART

Plasmid

#174804

Purpose

Bacterial expression of an inactive PARP1 mutant lacking the ART subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1deltaART (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 785-1014

Available Since

Oct. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1-CATdeltaHD

Plasmid

#174811

Purpose

Bacterial expression of a hyperactive PARP1 CAT lacking the autoinhibitory HD subdomain

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CATdeltaHD (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Residues 678-787 replaced by eight-residue linker…

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28_6xHis-PARP1delZn2delBRCT

Plasmid

#173939

Purpose

Bacterial expression of PARP1 lacking Zn2 and BRCT domains

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1delZn2delBRCT (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Deletion of residues 97-213 and 374-520; V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pOGS539_rPD-L1nb

Plasmid

#226717

Purpose

Plasmid enabling yeast-mediated expression and secretion of recombinant PD-L1 nanobody (rPD-L1nb)

Depositor

Gautam Dantas

Article

Rebeck et al Cell Chem Biol. 2024 Nov 16:S2451-9456(2

Insert

Recombinant PD-L1 nanobody

Tags

HA tag, Histidine tag, and Mating factor alpha se…

Expression

Yeast

Promoter

pTEF1

Available Since

Dec. 13, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_6xHis-PARP1-CAT-L713F

Plasmid

#173944

Purpose

Bacterial expression of isolated PARP1 CAT domain containing L713F gain-of-function mutation

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-CAT-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

L713F, V762A

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_6xHis-PARP1-L713F

Plasmid

#174794

Purpose

Bacterial expression of a hyperactive PARP1 mutant (L713F destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-L713F (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

L713F, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_6xHis-PARP1-C125G

Plasmid

#174792

Purpose

Bacterial expression of a less active PARP1 mutant (C125G reduces affinity for single-strand DNA break)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-C125G (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C125G, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a-Med25-VBD-6His

Plasmid

#64773

Purpose

Bacterial expression of His-tagged Med25-VBD

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

Med25-VBD (MED25 Human)

Expression

Bacterial

Mutation

insert is 394-543 only

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5-F-ELL2

Plasmid

#49422

Purpose

Mammalian expression of flag-tagged human ELL2

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

ELL2 (ELL2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5-Flag-EAF1

Plasmid

#49249

Purpose

Mammalian expression of flag-tagged human EAF1

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

EAF1 (EAF1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/FLAG-Ino80 ΔN (267-1556)

Plasmid

#29440

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 267-1556 (a.a.)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1-Y986H

Plasmid

#174801

Purpose

Bacterial expression of a PARP1 mutant that produces highly branched but short PAR chains overall

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Y986H (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Y986H, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_6xHis-PARP1-Y986S

Plasmid

#174802

Purpose

Bacterial expression of a PARP1 mutant that produces mainly short PAR oligomers

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-Y986S (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

Y986H, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_6xHis-PARP1-G972R

Plasmid

#174800

Purpose

Bacterial expression of a PARP1 mutant that produces less PAR oligomers overall

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-G972R (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

G972R, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5-Flag-EAF2

Plasmid

#49251

Purpose

Mammalian expression of flag-tagged human EAF2

Depositor

Joan Conaway, Ronald Conaway

Article

Takahashi et al Cell. 2011 Jul 8;146(1):92-104.

Insert

EAF2 (EAF2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Dec. 5, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/FLAG-Ino80 ΔN-EQ (267-1556)

Plasmid

#29438

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 267-1556 (a.a.) E653Q

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5FRT/Flag-hINO80 ΔN ΔHSA (520-1556)

Plasmid

#29446

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 520-1556 (a.a.)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/FLAG-Ino80 ΔNC (267-1261)

Plasmid

#29439

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 267-1261 (a.a.) with Q1116K mutation

Available Since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/FLAG-Ino80 ΔNC-EQ (267-1261)

Plasmid

#29437

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 267-1261 (a.a.) E653Q

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only
pET28_6xHis-PARP1-A774L

Plasmid

#174796

Purpose

Bacterial expression of a hyperactive PARP1 mutant (A774L destabilizes the autoinhibitory HD subdomain)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-A774L (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

A774L, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28_6xHis-PARP1-W318R

Plasmid

#174793

Purpose

Bacterial expression of an inactive PARP1 (W318R disrupts interdomain communication and HD subdomain unfolding)

Depositor

Joan Conaway

Article

Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

Insert

PARP1-W318R (PARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

W318, V762A

Available Since

Sept. 23, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5FRT/Flag-hINO80 HSA (212-526)

Plasmid

#29443

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 212-526 (a.a.)

Available Since

June 7, 2011

Availability

Academic Institutions and Nonprofits only
pET41s-GST-ATF6a-AD

Plasmid

#64774

Purpose

bacterial expression of GST-tagged ATF6 (1-150)

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

ATF6a-AD (1-150) (ATF6 Human)

Tags

GST

Expression

Mammalian

Mutation

C-term deletion

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5FRT/Flag-hINO80C (1224-1556)

Plasmid

#29445

Depositor

Joan Conaway, Ronald Conaway

Article

Chen et al J Biol Chem. 2011 Apr 1;286(13):11283-9.

Insert

INO80 (INO80 Human)

Tags

Flag

Expression

Mammalian

Mutation

INO80 1224-1556 (a.a.)

Available Since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5-Flag-Med25-VWA

Plasmid

#64772

Purpose

Mammalian expression of Flag-tagged Med25-VWA

Depositor

Joan Conaway, Ronald Conaway

Article

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

Med25-VWA (MED25 Human)

Tags

Flag

Expression

Mammalian

Mutation

C-terminal deletion

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pFB-Flag-MyoD(QQQ)-E12

Plasmid

#26003

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD(QQQ)-E12 Heterodimer Fusion (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Mutation

Changed Lysine 99 to Glutamine; Lysine 102 to Glu…

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge

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