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CAMK2A gRNA (BRDN0001162256)

Plasmid

#76929

Purpose

3rd generation lentiviral gRNA plasmid targeting human CAMK2A

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CAMK2A (CAMK2A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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EEF2K gRNA (BRDN0001162560)

Plasmid

#77856

Purpose

3rd generation lentiviral gRNA plasmid targeting human EEF2K

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EEF2K (EEF2K Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pMX-Flag-GFP_TMEM256

Plasmid

#227955

Purpose

Co-localisation experiment

Depositor

Alessandro Ori

Insert

TMEM256 (C17orf61 Human)

Use

Lentiviral

Tags

3xFLAG-EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin R256C

Plasmid

#227355

Purpose

For bacterial expression of hyperactive parkin mutant.

Depositor

Kalle Gehring

Article

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin R256C (PRKN Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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Salsa-BC256-P3F4

Plasmid

#203097

Purpose

Expression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC256

Depositor

Noah Palm

Article

Chen et al Cell. 2023 Jul 6;186(14):3095-3110.e19.

Insert

EGFP/BC256

Use

Tags

Expression

Mammalian

Mutation

Promoter

Tet Response Element (TRE)

Available since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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A0A1D1UNQ6_RAMVA pSecTag2(pm) (pBS0256)

Plasmid

#185145

Purpose

For the mammalian expression of the tardigrade protein A0A1D1UNQ6_RAMVA. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

Depositor

Pamela Silver

Article

Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

Insert

A0A1D1UNQ6_RAMVA

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pHAGE-EGFR-D256Y

Plasmid

#116236

Purpose

Lentiviral expression of EGFR D256Y

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

Expression

Mutation

D256Y

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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PRKX gRNA (BRDN0001162568)

Plasmid

#78013

Purpose

3rd generation lentiviral gRNA plasmid targeting human PRKX

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PRKX (PRKX Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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PFKFB2 gRNA (BRDN0001147256)

Plasmid

#77688

Purpose

3rd generation lentiviral gRNA plasmid targeting human PFKFB2

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PFKFB2 (PFKFB2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CDC42BPG gRNA (BRDN0001162561)

Plasmid

#77527

Purpose

3rd generation lentiviral gRNA plasmid targeting human CDC42BPG

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

CDC42BPG (CDC42BPG Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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MYO3A gRNA (BRDN0001146256)

Plasmid

#76226

Purpose

3rd generation lentiviral gRNA plasmid targeting human MYO3A

Depositor

David Root, John Doench

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

MYO3A (MYO3A Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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R777-E256 Hs.RASSF2-nostop

Plasmid

#70540

Purpose

Gateway ORF clone of human RASSF2 [NM_170774.1] without stop codon (for C-terminal fusions)

Depositor

Dominic Esposito

Insert

RASSF2 (RASSF2 Human)

Use

Gateway entry clone

Tags

Expression

Mutation

Promoter

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsTNRC6Biso1_1-1256_H

Plasmid

#146509

Purpose

Mammalian Expression of HsTNRC6Biso1_1-1256

Depositor

Elisa Izaurralde

Article

Eulalio et al RNA. 2009 Apr 21. ():.

Insert

HsTNRC6Biso1_1-1256 (TNRC6B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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puC19 preACT1(del6) C256A

Plasmid

#111331

Purpose

T7 transcription

Depositor

Christine Guthrie

Article

Villa et al Genes Dev. 2005 Aug 15;19(16):1894-904.

Insert

ACT1

Use

Tags

Expression

Bacterial

Mutation

branch site C256A

Promoter

Available since

Availability

Academic Institutions and Nonprofits only

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pSJ1256 (pFA6-link-yGFP1-10-CaURA3MX)

Plasmid

#86419

Purpose

C-terminal PCR tagging module for yeast codon optimized split GFP1-10

Depositor

Sue Jaspersen

Article

Smoyer et al J Cell Biol. 2016 Nov 21;215(4):575-590.

Insert

GFP1-10

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2017

Availability

Academic Institutions and Nonprofits only

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XE256 pCDNA3.1(zeo)-hDsh3-deltaCTER

Plasmid

#16753

Depositor

Randall Moon

Article

Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

Insert

FLAG-hDsh3-deltaCTER (DVL3 Human)

Use

Tags

flag

Expression

Mammalian

Mutation

delta CTER

Promoter

Available since

March 14, 2008

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC25A46[I349D]-His (SB256)

Plasmid

#227605

Purpose

Inducible expression of His-tagged SLC25A46 with I349D mutation in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Use

Tags

10xHis

Expression

Yeast

Mutation

I349D

Promoter

AOX1

Available since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIbleo-iRFP713(V256C)-P2A-EGFP

Plasmid

#198061

Purpose

The lentiviral vector for iRFP713(V256C) and EGFP

Depositor

Michiyuki Matsuda

Insert

iRFP713(V256C)

Use

Lentiviral

Tags

P2A-EGFP

Expression

Mammalian

Mutation

V256C mutation

Promoter

CAG

Available since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMH-HT_CtCHAD-H253A-R256A-R260A

Plasmid

#127788

Purpose

Expresses a CtCHAD mutant protein in E. coli cells

Depositor

Michael Hothorn

Article

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

CtCHAD (CT0884 Chlorobium tepidum)

Use

Tags

6xHis tag + TEV cleavage site

Expression

Bacterial

Mutation

Residues 208-522 (CHAD domain), mutations in His2…

Promoter

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pHBS1256 TDP43 RRM-GFP W385G

Plasmid

#107807

Purpose

To test the effect of sequence on TDP43 droplet properties

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

TARDBP mutant (TARDBP Human)

Use

Tags

Expression

Mammalian

Mutation

W385G mutation in LLPS reporter

Promoter

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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