We narrowed to 834 results for: Anti-CRISPR

1. pAAV-CMV-CASANOVA

   Plasmid

   #113036

   Purpose

   AAV vector for CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV CMV-driven AcrIIA4-2xmiR-122 target sites

   Plasmid

   #120298

   Purpose

   AAV vector for expression of AcrIIA4 with two miR-122 binding sites

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIA4-2xmiR-122 binding sites
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

3. AAV Intein-C-SpyCas9, CMV-AcrIIA4-scaffold (2xBsmBI sites)

   Plasmid

   #120294

   Purpose

   AAV Vector for expression of C-terminal SpyCas9 fragemnt with split-intein and a CMV-driven AcrIIA4 (no miR binding sites)

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   C-terminal fragment of SpyCas9
   

   Use

   AAV and CRISPR

   Tags

   split-intein

   Expression

   Mammalian

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV CMV-driven AcrIIA4-2xmiR-1 target sites

   Plasmid

   #120299

   Purpose

   AAV vector for expression of AcrIIA4 with two miR-1 binding sites

   Depositor

   Dominik Niopek

   Article

   Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

   Insert

   AcrIIA4-2xmiR-1 binding sites
   

   Use

   AAV and CRISPR

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Jan. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

   Plasmid

   #171129

   Purpose

   LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

   Depositor

   Benjamin Kleinstiver

   Article

   Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

   Insert

   U6 promoter entry plasmid for LwaCas13a crRNA cloning
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-T7-null (BPK1077)

   Plasmid

   #171128

   Purpose

   Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

   Depositor

   Benjamin Kleinstiver

   Article

   Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

   Insert

   pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)
   

   Use

   CRISPR

   Expression

   Bacterial and Mammalian

   Promoter

   pCMV and T7

   Available Since

   July 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

   Plasmid

   #129530

   Purpose

   Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Inserts

   codon-optimized AcrIIC3Nme
   sgRNA_Rosa26
   

   Use

   AAV

   Tags

   FLAG/NLS

   Promoter

   U6 and cytomegalovirus-enhancer chicken β-actin p…

   Available Since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

   Plasmid

   #129532

   Purpose

   Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Inserts

   codon-optimized AcrIIA4
   sgRNA_Rosa26
   

   Use

   AAV

   Tags

   FLAG/NLS

   Promoter

   U6 and cytomegalovirus-enhancer chicken β-actin p…

   Available Since

   Sept. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

   Plasmid

   #113039

   Purpose

   AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1-Puro CAG PSMA nanobody T-CAR

    Plasmid

    #171962

    Purpose

    Donor plasmid for PSMA nanobody T-CAR expression in human cells

    Depositor

    Xiaoping Bao

    Article

    Harris et al J Immunol Regen Med. 2023 May;20:100074.

    Insert

    Synthetic anti-PSMA nanobody CAR
    

    Use

    CRISPR, Synthetic Biology, and TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV Intein-C-SpyCas9, CMV-AcrIIA4-2xmiR-122 target sites

    Plasmid

    #120295

    Purpose

    AAV Vector for expression of C-terminal SpyCas9 fragement with split-intein and a CMV-driven AcrIIA4 with two miR-122 binding sites

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

    Insert

    C-terminal fragment of SpyCas9
    

    Use

    AAV and CRISPR

    Tags

    split-intein

    Expression

    Mammalian

    Available Since

    Jan. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-hCjeCas9-NLS(SV40)-3xFLAG (KAC579)

    Plasmid

    #133789

    Purpose

    CAG promoter expression plasmid for human codon optimized CjeCas9 nuclease with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized CjeCas9
    

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CAG

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)

    Plasmid

    #134331

    Purpose

    CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized Nme2Cas9
    

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEJS1026-pMCSG7-HpaCas9

    Plasmid

    #121540

    Purpose

    Expresses a 6X-His tagged type II-C Cas9 from H. parainfluenzae in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    HpaCas9
    

    Expression

    Bacterial

    Available Since

    Feb. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEJS1027-pMCSG7-SmuCas9

    Plasmid

    #121541

    Purpose

    Expresses a 6X-His tagged type II-C Cas9 from S. muelleri in bacterial cells

    Depositor

    Erik Sontheimer

    Article

    Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

    Insert

    SmuCas9
    

    Expression

    Bacterial

    Available Since

    Feb. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. p1193-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS-3XmiR122BS

    Plasmid

    #129531

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme with 3x miR-122 binding sites in 3' UTR

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIC3Nme
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)

    Plasmid

    #133788

    Purpose

    CAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized St3Cas9
    

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CAG

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-hNmeCas9-NLS(SV40)-3xFLAG (KAC409)

    Plasmid

    #133790

    Purpose

    CAG promoter expression plasmid for human codon optimized NmeCas9 nuclease with C-terminal NLS (SV40)

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized NmeCas9
    

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    CAG

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. CNC3 (CASANOVA-C3)

    Plasmid

    #137191

    Purpose

    Expresses CNC3 (CASANOVA-C3), a blue light dependent NmeCas9 inhibitory protein

    Depositor

    Dominik Niopek

    Article

    Hoffmann et al Nucleic Acids Res. 2021 Mar 18;49(5):e29

    Insert

    CNC3 (CASANOVA-C3)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcloDF-kan

    Plasmid

    #184837

    Purpose

    Backbone that confers Kanamycin resistance, contains cloDF ori

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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