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pET28a-ybbR-HIS-Xyl-DocI

Plasmid

#58711

Purpose

Characteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tag

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

Xyl-DocI

Use

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Mutation

changed Threonin 120 to Cysteine in Xylanase Doma…

Promoter

T7

Available since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

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PX458_TFE3_2

Plasmid

#86326

Purpose

Encodes gRNA for 3' target of human TFE3

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against TFE3 (TFE3 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_CIC_iso1

Plasmid

#135728

Purpose

Donor vector for 3' FLAG tag of human CIC_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CIC_iso1 Homology arms (CIC Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

March 18, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_NFIA_iso1_2

Plasmid

#104049

Purpose

Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

NFIA_iso1 gRNA (NFIA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_NFIA_iso1_1

Plasmid

#104048

Purpose

Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

NFIA_iso1 gRNA (NFIA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_CIC_iso1_2

Plasmid

#135744

Purpose

Encodes gRNA for 3' target of human CIC_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CIC_iso1 gRNA (CIC Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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PX458_CIC_iso1_1

Plasmid

#135743

Purpose

Encodes gRNA for 3' target of human CIC_iso1

Depositor

Richard M. Myers

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

CIC_iso1 gRNA (CIC Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pFETCh_RXRB

Plasmid

#104040

Purpose

Donor vector for 3' FLAG tag of human RXRB

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

RXRB homology arms (RXRB Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 23, 2018

Availability

Academic Institutions and Nonprofits only

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PX458_RXRB_1

Plasmid

#104050

Purpose

Encodes gRNA for 3' target of human RXRB along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

RXRB gRNA (RXRB Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_MBD1_iso1

Plasmid

#86263

Purpose

Donor vector for 3' FLAG tag of human MBD1_iso1

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

MBD1_iso1 homology arms (MBD1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_TEAD1

Plasmid

#86291

Purpose

Donor vector for 3' FLAG tag of human TEAD1

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

TEAD1 homology arms (TEAD1 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_RXRB_2

Plasmid

#104051

Purpose

Encodes gRNA for 3' target of human RXRB along with Cas9 with 2A GFP

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

RXRB gRNA (RXRB Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_MBD1_iso1_2

Plasmid

#86304

Purpose

Encodes gRNA for 3' target of human MBD1_iso1

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against MBD1_iso1 (MBD1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_GATAD2A_2

Plasmid

#86334

Purpose

Encodes gRNA for 3' target of human GATAD2A

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against GATAD2A (GATAD2A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_GATAD2A_1

Plasmid

#86333

Purpose

Encodes gRNA for 3' target of human GATAD2A

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against GATAD2A (GATAD2A Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_MBD1_iso1_1

Plasmid

#86303

Purpose

Encodes gRNA for 3' target of human MBD1_iso1

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against MBD1_iso1 (MBD1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_ZNF652

Plasmid

#86270

Purpose

Donor vector for 3' FLAG tag of human ZNF652

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

ZNF652 homology arms (ZNF652 Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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PX458_HLF_2

Plasmid

#86302

Purpose

Encodes gRNA for 3' target of human HLF

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HLF (HLF Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Feb. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pFETCh_HLF

Plasmid

#86262

Purpose

Donor vector for 3' FLAG tag of human HLF

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

HLF homology arms (HLF Human)

Use

CRISPR

Tags

3XFLAG-P2A-NeoR

Expression

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
PX458_HLF_1

Plasmid

#86301

Purpose

Encodes gRNA for 3' target of human HLF

Depositor

Richard M. Myers, Eric Mendenhall

Article

Savic et al Genome Res. 2015 Sep 9.

Insert

gRNA against HLF (HLF Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge