Addgene

Showing: 141 - 160 of 10226 results

Results per page:

Sort results by:

T7-15-QUAS-GFP_ColE1

Plasmid

#171658

Purpose

QUAS fifteen base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-15-QUAS

Use

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
QUAS-0-T7-GFP_ColE1

Plasmid

#171649

Purpose

QUAS directly upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

QUAS-0-T7

Use

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-0-QUAS-GFP_ColE1

Plasmid

#171650

Purpose

QUAS directly downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-0-QUAS

Use

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
QUAS-0-T7-CcdB_ColE1

Plasmid

#171652

Purpose

QUAS directly upstream of a T7 promoter. Expresses CcdB in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

CcdB-ssrA

Use

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
QUAS-5-T7-GFP_ColE1

Plasmid

#171653

Purpose

QUAS five base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

QUAS-5-T7

Use

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
QUAS-10-T7-GFP_ColE1

Plasmid

#171654

Purpose

QUAS ten base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

QUAS-10-T7

Use

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
QUAS-15-T7-GFP_ColE1

Plasmid

#171655

Purpose

QUAS fifteen base pairs upstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

QUAS-15-T7

Use

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
T7-5-QUAS-GFP_ColE1

Plasmid

#171656

Purpose

QUAS five base pairs downstream of a T7 promoter. Expresses GFP in BL21(DE3) E. coli. Contains ColE1 origin of replication.

Depositor

Tara Deans

Article

MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:

Insert

T7-5-QUAS

Use

Tags

ssrA degradation tag (DAS+4)

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pET30-T7-lacO-sfGFP

Plasmid

#180756

Purpose

pET30 vector missing N-terminal His tag with sfGFP-S-tag inserted between the Bgl II and T7 terminator

Depositor

Michael Black

Article

Jew et al Front Bioeng Biotechnol. 2022 Jun 23;10:

Insert

superfolder green fluorescent protein

Use

Tags

strep tag

Expression

Bacterial

Mutation

Promoter

Available since

July 19, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-RiboJ-mTFP1-T7

Plasmid

#173225

Purpose

Encodes mTFP fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

mTFP1

Use

Cell-free system

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-T7-null (BPK1077)

Plasmid

#171128

Purpose

Plasmid encoding a pCMV-T7 with a 2 amino acid peptide prior to a stop codon.

Depositor

Benjamin Kleinstiver

Article

Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

Insert

pCMV-T7 null expression plasmid that encodes a two amino acid peptide (Met-Asp)

Use

CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

pCMV and T7

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pHCKan-T7-CBD-hGLY

Plasmid

#134940

Purpose

T7 based expression of human glycine acyltransferase with an N-terminal chitin binding domain

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

human glycine acyltransferase 2 (GLYATL2 )

Use

Tags

chitin binding domain

Expression

Bacterial

Mutation

Promoter

t7

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET-20b-T7-Ntx

Plasmid

#58714

Purpose

Expresses T7-Notexin in Escherichia coli

Depositor

Fiorella Tonello

Article

Simonato et al Toxicon. 2014 Jun 18. pii: S0041-0101(14

Insert

Notexin

Use

Tags

T7 peptide tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 T7 BimL (S44D)

Plasmid

#24530

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(S44D) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Changed serine 44 to aspartic acid

Promoter

Available since

Aug. 13, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL (AA)

Plasmid

#24243

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(AA) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

changed Threonine 56 to Alanine changed Serine …

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL (AAA)

Plasmid

#24244

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(AAA) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

changed Serine 44 to Alanine changed Threonine 5…

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL (T56A)

Plasmid

#24247

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

Bim L(T56A) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Changed threonine 56 to alanine

Promoter

Available since

June 1, 2010

Availability

Academic Institutions and Nonprofits only
pCDNA3 T7 BimL (S44A)

Plasmid

#24245

Depositor

Roger Davis

Article

Lei et al Proc Natl Acad Sci U S A. 2003 Mar 4. 10

Insert

BimL (S44A) (BCL2L11 Human)

Use

Tags

T7

Expression

Mammalian

Mutation

Changed Serine 44 to Alanine

Promoter

Available since

May 10, 2010

Availability

Academic Institutions and Nonprofits only
pUC19 - T7 pro - IRES - EGFP

Plasmid

#138586

Purpose

Express EGFP with an upstream T7 promoter in pUC19 backbone

Depositor

Fei Chen

Article

Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

EGFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ICMAB - pET22b T7 EncFtn Mms6

Plasmid

#225538

Purpose

Construction of intracellular magnetite accumulating bacterial systems.

Depositor

Urartu Seker

Article

Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

Inserts

Iron binding protein
Ferroxidase
Material binding peptite
Iron transporter protein

Use

Tags

no

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge