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(194) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4,K852A)

Plasmid

#160742

Purpose

Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to the EPEA nanobody. A different nanobody can be inserted by using Sgfl + Sgsl restriction enzymes.

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

HA-nEPEA-(EAAAK)4-OGT(4,K852A)

Use

Tags

HA-Tag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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(282) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4,K852A)

Plasmid

#160741

Purpose

Expresses an inactive truncated OGT (4.5 TPRs, K852A) that can't bind O-GlcNAc fused to a GFP nanobody

Depositor

Christina Woo

Article

Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

Insert

HA-nGFP-(EAAAK)4-OGT(4,K852A)

Use

Tags

HA-Tag

Expression

Mammalian

Mutation

Promoter

T7/CMV

Available since

May 25, 2021

Availability

Academic Institutions and Nonprofits only

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pQlinkG2-PTPIP51(236-470)

Plasmid

#170534

Purpose

Expresses Cleavable GST-tagged PTPIP51 TPR domain (236-470) in E. Coli

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (RMDN3 Human)

Use

Tags

GST tag

Expression

Bacterial

Mutation

Promoter

T5 promoter

Available since

Aug. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pQlinkH-PTPIP51(236-470)

Plasmid

#170530

Purpose

Expresses Cleavable His-tagged PTPIP51 TPR domain (236-470) in E. Coli

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (236-470) (RMDN3 Human)

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T5 promoter

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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pLX302-PTP-SL-V5 puro

Plasmid

#87774

Purpose

Expresses V5 tagged human PTP-SL

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

PTP-SL (PTPRR Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2017

Availability

Academic Institutions and Nonprofits only

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pFlare9A-CD45i4e5i5

Plasmid

#131834

Purpose

A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a CTCF binding site.

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

PTPRC (PTPRC Human)

Use

Reporter construct

Tags

Expression

Mutation

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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pPN204

Plasmid

#91655

Purpose

Express sgRNA targeting human PTPRF

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

PTPRF (PTPRF Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN205

Plasmid

#91656

Purpose

Express sgRNA targeting human PTPRF

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

PTPRF (PTPRF Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFlare9A-CD45i4e5i5mutCBS

Plasmid

#131835

Purpose

A reporter construct containing the Exon 5 of PTPRC (CD45), which contains a mutant CTCF binding site, making the exon to be always excluded.

Depositor

Mark Minden

Article

Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

Insert

PTPRC (PTPRC Human)

Use

Tags

Expression

Mammalian

Mutation

partial intron 4 full exon 5 and partial intron 5…

Promoter

Available since

May 3, 2022

Availability

Academic Institutions and Nonprofits only

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TFORF1275

Plasmid

#143781

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TPRX1 (TPRX1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1 hSCN5AdeltaSIV GFP Nter

Plasmid

#145770

Purpose

expresses human Nav1.5 lacking the SIV motif and tagged with GFP at its N-terminus in mammalian cells

Depositor

Hugues Abriel

Article

Petitprez et al Circ Res. 2011 Feb 4;108(3):294-304. doi

Insert

sodium voltage-gated channel alpha subunit 5 (SCN5A Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Stop codon at Serine 2013

Promoter

CMV

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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HT PTP-RN

Plasmid

#24056

Depositor

Frederick Stanley

Insert

PTPRN (PTPRN Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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HT PTP-RN 695/979

Plasmid

#24057

Depositor

Frederick Stanley

Insert

PTPRN (PTPRN Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Only contains aa 695-979

Promoter

Available since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only
pTPTP alpha

Plasmid

#17691

Depositor

Xinmin Zheng

Article

Zheng et al EMBO J. 2000 Mar 1. 19(5):964-78.

Insert

PTPalpha (PTPRA Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pNTPTP alpha

Plasmid

#17696

Depositor

Xinmin Zheng

Article

Zheng et al EMBO J. 2000 Mar 1. 19(5):964-78.

Insert

PTPalpha (PTPRA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pTPTP alpha (Y789F)

Plasmid

#17693

Depositor

Xinmin Zheng

Article

Zheng et al EMBO J. 2000 Mar 1. 19(5):964-78.

Insert

PTPalpha Y789F (PTPRA Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Y789F

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pTPTP alpha (CCSS)

Plasmid

#17692

Depositor

Xinmin Zheng

Article

Zheng et al EMBO J. 2000 Mar 1. 19(5):964-78.

Insert

PTPalpha CCSS (PTPRA Human)

Use

Tags

HA

Expression

Mammalian

Mutation

C433S / C723S

Promoter

Available since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pNTPTP alpha (Y789F)

Plasmid

#17702

Depositor

Xinmin Zheng

Article

Zheng et al EMBO J. 2000 Mar 1. 19(5):964-78.

Insert

PTPalpha Y789F (PTPRA Human)

Use

Tags

Expression

Mammalian

Mutation

Y789F

Promoter

Available since

April 7, 2008

Availability

Academic Institutions and Nonprofits only
pSAM_Ec

Plasmid

#102939

Purpose

Transposon mutagenesis and Tn-seq

Depositor

Matthew Mulvey

Article

Wiles et al PLoS Genet. 2013;9(8):e1003716. doi: 10.

Insert

mariner transposon flanked by MmeI modified inverted repeats and the himar1C9 transposase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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phR-Luc

Plasmid

#139644

Purpose

Mammalian gene expression

Depositor

Markku Varjosalo

Article

Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

Insert

Renilla luciferase

Use

Luciferase

Tags

Expression

Mutation

Promoter

SV40

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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