We narrowed to 6,904 results for: crispr cas9 plasmids

1. pX330-U6-BRD4-HaloTag-Guide-hspCas9

   Plasmid

   #228576

   Purpose

   Expression of Halo-tagged human BRD4 gRNA; CRISPR-mediated gene insertion

   Depositor

   Marcin Imielinski

   Insert

   BRD4 gRNA (BRD4 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CMV-FLEX-SaCas9-U6-sgPenk

   Plasmid

   #159916

   Purpose

   Mutagenesis of Penk

   Depositor

   Larry Zweifel

   Insert

   Penk (Penk Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-CMV-FLEX-SaCas9-U6-sgSlc32a1

   Plasmid

   #159905

   Purpose

   Mutagenesis of Slc32a1

   Depositor

   Larry Zweifel

   Insert

   Slc32a1 (Slc32a1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-CMV-FLEX-SaCas9-U6-sgKcnn3

   Plasmid

   #159904

   Purpose

   Mutagenesis of Kcnn3

   Depositor

   Larry Zweifel

   Insert

   Kcnn3 (Kcnn3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CMV-FLEX-SaCas9-U6-sgKcnq3

   Plasmid

   #159911

   Purpose

   Mutagenesis of Kcnq3

   Depositor

   Larry Zweifel

   Insert

   Kcnq3 (Kcnq3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CMV-FLEX-SaCas9-U6-sgDrd2

   Plasmid

   #159918

   Purpose

   Mutagenesis of Drd2

   Depositor

   Larry Zweifel

   Insert

   Drd2 (Drd2 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CMV-FLEX-SaCas9-U6-sgDrd1

   Plasmid

   #159917

   Purpose

   Mutagenesis of Drd1

   Depositor

   Larry Zweifel

   Insert

   Drd1 (Drd1 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

   Plasmid

   #159912

   Purpose

   Mutagenesis of Kcnd3

   Depositor

   Larry Zweifel

   Insert

   Kcnd3 (Kcnd3 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6

   Plasmid

   #159913

   Purpose

   Mutagenesis of Kcnj6

   Depositor

   Larry Zweifel

   Insert

   Kcnj6 (Kcnj6 Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pdCas9-M-G6

    Plasmid

    #73437

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant G6.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor G6 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available since

    March 3, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pdCas9-M-C4

    Plasmid

    #73427

    Purpose

    CRISPR synthetic transcription factor repressor plasmid encoding dCas9, tracrRNA, and a single spacer CRISPR array encoding crRNA for orthogonal repression of T7-lac promoter variant C4.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Repressor C4 (orthogonal T7-lac repressor)
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Constitutive wild-type S. pyogenes promoter

    Available since

    April 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. lenti-dCas9-VP64-BFP

    Plasmid

    #196711

    Purpose

    CRISPR-activation. dCas9-VP64 with TagBFP for sorting

    Depositor

    Bernhard Schmierer

    Insert

    dCas9-VP64-T2A-TagBFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mutation

    D10A, N863A

    Promoter

    EF1a

    Available since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. p-dCas9-SET7-Hygro

    Plasmid

    #104407

    Purpose

    transient expression of dCas9-SET7 fusion protein

    Depositor

    Stephan Beck

    Insert

    SET7 (SETD7 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJAK184.tetR.PT5-3.dCas9

    Plasmid

    #232482

    Purpose

    optimized ahTet inducible dCas9 for insertion into C. difficile genome

    Depositor

    James Collins

    Article

    Chua et al Microbiol Resour Announc. 2025 Apr 28:e0

    Insert

    Catalytically dead mutant of the Cas9 endonuclease from the Streptococcus pyogenes Type II CRISPR/Cas system
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    TetR-PT5-3

    Available since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. p-dCas9-p300-S1396R-S1397R-Hygro

    Plasmid

    #104419

    Purpose

    transient expression of dCas9-dp300 fusion protein

    Depositor

    Stephan Beck

    Insert

    dp300
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    S1396R-S1397R

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUB-Cas9

    Plasmid

    #86556

    Purpose

    For ubiquitous expression of Cas9 in planta. sgRNA can be introduced as described in Hahn et al. (2017)

    Depositor

    Andreas Weber

    Article

    Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Cas9 is codon optimized for C. reinhardtii

    Promoter

    UBIQUITIN10

    Available since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PtPuc3_diaCas9_sgRNA

    Plasmid

    #109219

    Purpose

    Episome based vector with CRISPR/Cas9_sgRNA module for genome editing in Phaeodactylum tricornutum

    Depositor

    Per Winge

    Article

    Sharma et al Sci Rep. 2018 Sep 26;8(1):14401. doi: 10

    Inserts

    LHCF2 promoter
    diaCas9
    LHCF1 terminator
    U6 promoter
    sgRNA
    U6 3' region
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    LHCF1 terminator, LHCF2 promoter, U6 3' regi…

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. CROPseq-EFS-SpCas9-P2A-blast

    Plasmid

    #106477

    Purpose

    single-vector CROPseq system with blasticidin resistance selection for use in the CROPseq CRISPR knockout screens

    Depositor

    Alex Hewitt

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    April 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti_dCas9-2xAM

    Plasmid

    #92220

    Purpose

    Lentiviral plasmid expressing dCas9-2xAM and gRNA cloned in Bbs I sites

    Depositor

    Hodaka Fujii

    Article

    Fujita et al BMC Res Notes. 2018 Feb 27;11(1):154. do

    Inserts

    Sp-dCas9-2xAM tag
    gRNA to be inserted into Bbs I sites
    

    Use

    CRISPR and Lentiviral

    Tags

    2xAM tag

    Expression

    Mammalian

    Mutation

    human codon-optimized, D10A + H840A

    Promoter

    CBh and U6

    Available since

    June 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. p-dCas9-dDNMT3a-C706S-Hygro

    Plasmid

    #104412

    Purpose

    transient expression of dCas9-dDNMT3a fusion protein

    Depositor

    Stephan Beck

    Insert

    dDNMT3a
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    C706S

    Promoter

    CMV promoter

    Available since

    April 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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