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Plasmid#77983Purpose3rd generation lentiviral gRNA plasmid targeting human BRD2DepositorInsertBRD2 (BRD2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
BRD2 gRNA (BRDN0001148924)
Plasmid#77984Purpose3rd generation lentiviral gRNA plasmid targeting human BRD2DepositorInsertBRD2 (BRD2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
BRD4 gRNA (BRDN0001145474)
Plasmid#77347Purpose3rd generation lentiviral gRNA plasmid targeting human BRD4DepositorInsertBRD4 (BRD4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
BRD4 gRNA (BRDN0001146786)
Plasmid#77346Purpose3rd generation lentiviral gRNA plasmid targeting human BRD4DepositorInsertBRD4 (BRD4 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
BRD2 gRNA (BRDN0001145548)
Plasmid#77986Purpose3rd generation lentiviral gRNA plasmid targeting human BRD2DepositorInsertBRD2 (BRD2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
BRD2 gRNA (BRDN0001148966)
Plasmid#77985Purpose3rd generation lentiviral gRNA plasmid targeting human BRD2DepositorInsertBRD2 (BRD2 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6Available sinceJuly 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
10xHis-3C-BRD4(42-459) BD1-BD2
Plasmid#233886PurposepRSF-DUET1 for protein overexpression of human BRD4DepositorInsertBRD4 (BRD4 Human)
UseTags10x his 3CExpressionBacterialMutationPromoterT7Available sinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Hb9-CD14
Plasmid#204344PurposeKnock-in vector to insert a motor neuron-specific MACS-sortable genetic reporter into the AAVS1 locus in human pluripotent stem cells. Allows isolation of human iPSC-derived motor neurons.DepositorInserthuman CD14 (CD14 Human)
UseTagsExpressionMammalianMutationPromotermouse Hb9 (Mnx1) 9kb promoter fragmentAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pD649-HAsp-NRG2-Fc(DAPA)-AviTag-6xHis
Plasmid#156734PurposeMammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.DepositorInsertNRG2 (NRG2 Human)
UseTagsFc(DAPA)-AviTag-6xHisExpressionMammalianMutationFc region of human IgG contains D265A and P329A m…PromoterCMV/SP6Available sinceMarch 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BRD4_WT
Plasmid#82323PurposeGateway Donor vector containing BRD4 , part of the Target Accelerator Plasmid Collection.DepositorInsertBRD4 (BRD4 Human)
UseGateway entry vectorTagsExpressionMutationP474LPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT
Plasmid#204694PurposeThis plasmid allows for inducible expression of full-length BRD4-NUT fusion tagged with mNeonGreen, in mammalian cells.DepositorUseTagsmNeonGreenExpressionMammalianMutationdirect fusion of BRD4 and NUTPromoterCMVAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mCherry-2A-BRD4 Iso AdelCTD
Plasmid#137722PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)DepositorInsertBRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationTruncated at amino acid 1328PromoterTight TRE promoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4-p300
Plasmid#204697PurposeThis plasmid allows for inducible expression of engineered BRD4-p300 fusion tagged with mNeonGreen, in mammalian cells.DepositorUseTagsmNeonGreenExpressionMammalianMutationdirect fusion of BRD4 to p300PromoterCMVAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57-mCherry-2A-BRD4 Iso CdelET
Plasmid#137723PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)DepositorInsertBRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationDeleted amino acids 600-682PromoterTight TRE promoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
His-GST-TEV-BRD4(42-459) BD1-BD2
Plasmid#233887PurposepGex for protein overexpression of human BRD4DepositorInsertBRD4 (BRD4 Human)
UseTagsHis-GST-TEVExpressionBacterialMutationPromoterT7Available sinceMarch 11, 2025AvailabilityAcademic Institutions and Nonprofits only -
KHC065 pBABE pU6 BRD2 K1-2C
Plasmid#231712PurposeKHC065 (pBABE pU6 BRD2 K1-2C), gRNA and cas9D10A mammalian expression vector for CRISPR mediated cutting of BRD2 at the N-terminus, use with KHC064DepositorInsertgRNA for Brd2 and Cas9D10A (BRD2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
KHC064 pX335 BRD2 K1-1a
Plasmid#231711PurposeKHC064 (pX335 BRD2 K1-1a), gRNA and cas9D10A mammalian expression vector for CRISPR mediated cutting of BRD2 at the N-terminus, use with KHC065DepositorInsertgRNA for Brd2 and Cas9D10A (BRD2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
KHC068 BRD2-KI-GFP-2A-L387A
Plasmid#231713PurposeCRISPR mediated KI of BromoTag at N-terminus of BRD2-GFP-P2A-BromoTag, works well with KHC064 and KHC065DepositorInsertBrd2 (BRD2 Human)
UseCRISPRTagsBromoTag and GFPExpressionMammalianMutationL387APromoterAvailable sinceFeb. 19, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4-p300(deltaI)
Plasmid#204701PurposeThis plasmid allows for inducible expression of engineered BRD4-p300(I) fusion tagged with mNeonGreen, in mammalian cells.DepositorUseTagsmNeonGreenExpressionMammalianMutationdeletion of intrinsically disordered regions of p…PromoterCMVAvailable sinceSept. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-mNeonGreen-BRD4-NUT(deltaMIN)
Plasmid#204696PurposeThis plasmid allows for inducible expression of BRD4-NUT(deltaMIN) mutant fusion tagged with mNeonGreen, in mammalian cells.DepositorUseTagsmNeonGreenExpressionMammalianMutationdeletion of the amino acids 355-505 of NUTPromoterCMVAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only