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pAPInt-mNeonGreen-Hyg

Plasmid

#236483

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Hyg.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-mNeonGreen-Nat

Plasmid

#236482

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with mNeonGreen and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al bioRxiv [Preprint]. 2025 Jan 27:2025.01.

Insert

mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Promoter

Available since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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lenti_U6_miR-Oct4e_TRE12_OCT4(co)-mNeonGreen_WPRE

Plasmid

#229544

Purpose

Lentiviral construct to express synthetic miRNA and OCT4-mNeonGreen fusion protein

Depositor

Domitilla Del Vecchio

Article

Ilia et al Sci Adv. 2023 Dec;9(48):eadg8495. doi: 1

Insert

Oct4(co)-mNeonGreen (POU5F1 Human)

Use

Lentiviral

Tags

Oct4-mNeonGreen

Expression

Mammalian

Mutation

codon-optimized OCT4

Promoter

TRE12

Available since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pJM220-J23100-mNeongreen

Plasmid

#210866

Purpose

Tn7 insertion plasmid for Pseudomonas synxantha 2-79. Inserts mNeongreen driven by the J23100 promoter

Depositor

Richard Murray

Article

Meyerowitz et al ACS Synth Biol. 2024 Feb 16;13(2):530-53

Insert

mNeongreen

Use

Tags

Expression

Bacterial

Mutation

Promoter

J23100

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pJM220-Syn35-mNeongreen

Plasmid

#210867

Purpose

Tn7 insertion plasmid for Pseudomonas synxantha 2-79. Inserts mNeongreen driven by the Syn35 promoter.

Depositor

Richard Murray

Article

Meyerowitz et al ACS Synth Biol. 2024 Feb 16;13(2):530-53

Insert

mNeongreen

Use

Tags

Expression

Bacterial

Mutation

Promoter

Syn35

Available since

Dec. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-HBEGF-mNeonGreen

Plasmid

#209912

Purpose

To monitor the expression of HB-EGF, the plasmid encodes a recombinant HB-EGF fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

HBEGF-mNeonGreen (HBEGF Human)

Use

Lentiviral

Tags

mNeonGreen

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-TGFa-mNeonGreen

Plasmid

#209913

Purpose

To monitor the expression of TGFα, the plasmid encodes a recombinant TGFα fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

TGFa-mNeonGreen (TGFA Human)

Use

Lentiviral

Tags

mNeonGreen

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pCSIIpuro-EREG-mNeonGreen

Plasmid

#209914

Purpose

To monitor the expression of Epiregulin, the plasmid encodes a recombinant Epiregulin fused intracellularly to the mNeonGreen fluorescent protein

Depositor

Michiyuki Matsuda

Article

Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

Insert

EREG-mNeonGreen (EREG Human)

Use

Lentiviral

Tags

mNeonGreen

Expression

Mutation

Promoter

Available since

Nov. 18, 2024

Availability

Academic Institutions and Nonprofits only

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CUP1-∆ssCPY*-mNeonGreen

Plasmid

#221156

Purpose

Fluorescent reporter for cytoplasmic aggregates

Depositor

Mark Leake

Insert

Carboxypeptidase Y (PRC1 Budding Yeast)

Use

Tags

mNeonGreen

Expression

Yeast

Mutation

Deletion signal peptide, G255A mutation

Promoter

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pPtet-1_mNeonGreen_Ptet-2_mCherry

Plasmid

#213839

Purpose

Expression of mNeonGreen and mCherry from aTc-inducible Ptet promoters.

Depositor

James Collins

Article

Alcantar et al Mol Cell. 2024 Jun 20;84(12):2382-2396.e

Insert

mNeonGreen, mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_MSX1-FKBP-mNeonGreen-V5

Plasmid

#215025

Purpose

AAV vector for knocking in a C-terminal tag (FKBP12F36V, mNeonGreen, and V5) for human MSX1

Depositor

Joanna Wysocka

Article

Kim et al Cell. 2024 Feb 1;187(3):692-711.e26. doi

Insert

msh homeobox 1 (MSX1 Human)

Use

AAV

Tags

FKBP-mNeonGreen-V5

Expression

Mutation

Promoter

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLSU1/o35S[]:mNeonGreen:NosT

Plasmid

#212161

Purpose

This binary vector expresses mNeonGreen with the original 35sCAMV promoter

Depositor

Wayne Curtis

Insert

Tomato codon optimized version of mNeonGreen

Use

Tags

Expression

Plant

Mutation

mNeonGreen codon-optimized for tomato

Promoter

original 35sCaMV

Available since

March 6, 2024

Availability

Academic Institutions and Nonprofits only

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ALIX(P801G)-mNeonGreen

Plasmid

#191192

Purpose

Full-length ALIX with P801G mutation in proline-rich domain; C-terminally tagged with mNeonGreen.

Depositor

Jin Zhang

Article

Elias et al Sci Adv. 2023 Jul 14;9(28):eadg3913. doi

Insert

ALIX (P801G) (PDCD6IP Human, Synthetic)

Use

Tags

6xHIS and mNeonGreen

Expression

Mammalian

Mutation

Pro 801 mutated to Gly

Promoter

CMV

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-DARPinOFF7

Plasmid

#182414

Purpose

Bacterial expression of mNeongreen-DARPinOFF7 fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-DARPinOFF7

Use

Tags

mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-Uricase

Plasmid

#182416

Purpose

Bacterial expression of mNeongreen-uricase fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-uricase

Use

Tags

mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-StefinA

Plasmid

#182418

Purpose

Bacterial expression of mNeongreen-stefinA fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-stefin (CSTA Human)

Use

Tags

mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_mNeongreen-Erythropoietin

Plasmid

#182422

Purpose

Bacterial expression of mNeongreen-Erythropoietin fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

mNeongreen-eythropoietin (EPO Human)

Use

Tags

mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMKRQ_11_DExCon-mNeonGreen-R25

Plasmid

#179896

Purpose

Donor with homologous arms for Rab25 to knock in DExCon-mNeonGreen module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB25, member RAS oncogene family (RAB25 Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

Promoter

TRE3GS

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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ymTq2-ymNeongreen pDRF1-GW

Plasmid

#168069

Purpose

FRET fusion between ymTq2 and ymNeongreen for use in yeast

Depositor

Bas Teusink

Insert

ymTq2-ymNeongreen

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 18, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-UbC-mNeonGreen

Plasmid

#124102

Purpose

Expression of the fluorescent protein mNeonGreen in mammalian cells

Depositor

Pantelis Tsoulfas

Insert

mNeonGreen

Use

AAV

Tags

Expression

Mammalian

Mutation

Codon usage was optimized for Homo Sapiens

Promoter

UbC

Available since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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