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ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor

Plasmid

#173208

Purpose

HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

ATP1A1-T804N mScarlet-I-TOSI donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hPGK1

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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PMT-mScarlet_2

Plasmid

#203780

Purpose

Brightness calibration

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

PMT-mScarlet dimer

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2023

Availability

Academic Institutions and Nonprofits only

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H11-mScarletLAMA4

Plasmid

#172439

Purpose

Hipp11 targeting vector, expresses mScarlet-mLAMA4

Depositor

Tyler Jacks

Article

Freed-Pastor et al Cancer Cell. 2021 Oct 11;39(10):1342-136

Insert

mScarlet-mLAMA4

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

June 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDx_mScarlet-SYFP2

Plasmid

#189762

Purpose

Dual expression vector for bacteria and mammalian cells producing mScarlet-SYFP2 fusion protein for FRET analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-SYFP2

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn_CapChR1_mScarlet

Plasmid

#188037

Purpose

Expresses CapChR1-mScarlet in mammalian neurons

Depositor

Peter Hegemann

Article

Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

Insert

CapChR1-mScarlet

Use

AAV

Tags

mScarlet

Expression

Mutation

S63D, L132C, T159C and N258E mutations in CrChR2

Promoter

hSyn

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pIN3_enpp-1_mScarlet

Plasmid

#182934

Purpose

Expresses enpp-1 (C27A7.1) under its native promoter (male reproductive tract and ciliated neurons IL2, CEMs, HOB, RnBs in C. elegans)

Depositor

Maureen Barr

Article

Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

Insert

genomic enpp-1 fused to mScarlet

Use

Tags

flexible linker (GGGGS)x3 and mScarlet tag

Expression

Worm

Mutation

Promoter

enpp-1

Available since

Sept. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pMT335-mScarletI

Plasmid

#174506

Purpose

High-copy number plasmid (pMT335) harboring the mScarletI gene for its cytoplasmic expression, inducible by vanillate (sequence of mScarletI optimized)

Depositor

Suliana Manley

Article

Mahecic et al Nat Methods. 2022 Sep 8. pii: 10.1038/s4

Insert

mScarletI

Use

Tags

mScarletI

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pIN2_mcm-3_mScarlet

Plasmid

#182933

Purpose

Expresses mcm-3 under its native promoter (embryonic nuclei and ciliated neurons IL2, CEMs, HOB, RnBs in C. elegans)

Depositor

Maureen Barr

Article

Nikonorova et al Curr Biol. 2022 May 9;32(9):1924-1936.e6

Insert

genomic mcm-3 fused to mScarlet

Use

Tags

flexible linker (GGGGS)x3 and mScarlet tag

Expression

Worm

Mutation

Promoter

mcm-3

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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RPL13A_PQR_mScarlet_NOL_LoxP_PQR_zeo_PQR_MCS_RPL13A-3'UTR

Plasmid

#165043

Purpose

Repair template for CAS9 complex genome editing

Depositor

Brian Chen

Article

Lo et al Biotechniques. 2017 Apr 1;62(4):165-174.

Insert

RPL13A (RPL13A Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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68Q-ymScarlet

Plasmid

#128562

Purpose

Httex1 (without proline-rich region) fused to ymScarlet

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

68Q-Httex1 (without proline-rich region) fused to ymSarlet

Use

Tags

ymScarlet

Expression

Yeast

Mutation

Promoter

Gal1

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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25Q-ymScarlet

Plasmid

#128561

Purpose

Httex1 (without proline-rich region) fused to ymScarlet

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

25Q-Httex1 (without proline-rich region) fused to ymSarlet

Use

Tags

ymScarlet

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pTU1-A-SP44-mScarlet-I

Plasmid

#163756

Purpose

Expresses Streptomyces codon-optimised mScarlet-I

Depositor

Paul Freemont, Simon Moore

Article

Moore et al ACS Synth Biol. 2021 Feb 19;10(2):402-41

Insert

mScarlet-I

Use

Synthetic Biology

Tags

Flag and FlAsH tags

Expression

Bacterial

Mutation

Promoter

SP44

Available since

July 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2FA-H2B-mScarlet3

Plasmid

#224436

Purpose

Construct for labelling cell nuclei in mammalian cells using Histon protein 2B fused to mScarlet3

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

H2B-mScarlet3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV & SP6

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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PGK mScarlet-Synaptophysin

Plasmid

#206145

Purpose

PGK mScarlet-Synaptophysin

Depositor

Erika Holzbaur

Article

Dou et al J Cell Biol. 2024 Jun 3;223(6):e20230709

Insert

synaptophysin (Syp Rat)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

PGK

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV_3xFLAG-ATP6V0A3-mScarlet

Plasmid

#228867

Purpose

Stable expression of V-ATPase subunit ATP6V0A3 with N-terminal 3xFLAG and C-terminal mScarlet

Depositor

Wilhelm Palm

Article

Ratto et al Nat Commun. 2022 Aug 17;13(1):4848. doi:

Insert

ATP6V0A3 (TCIRG1 Human)

Use

Lentiviral

Tags

3xFLAG and mScarlet

Expression

Mutation

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA-Malibu-mScarlet

Plasmid

#229554

Purpose

Fluorescent reporter for malonyl-CoA

Depositor

Danielle Schmitt

Article

Ranzau et al bioRxiv [Preprint]. 2024 Sep 28:2024.09.

Insert

Malibu-mScarlet

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA-deadMalibu-mScarlet

Plasmid

#229555

Purpose

Fluorescent reporter for malonyl-CoA that does not bind malonyl-CoA (control)

Depositor

Danielle Schmitt

Article

Ranzau et al bioRxiv [Preprint]. 2024 Sep 28:2024.09.

Insert

Malibu-mScarlet

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet3

Plasmid

#189752

Purpose

Produces an mTurquoise-antiFRET-mScarlet3 fusion protein in bacteria and in mamallian cells it produces 1:1 unfused mTurquoise2 and mScarlet3 FPs for ratiometric analysis

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mTurquoise2_spatial-linker-P2A_mScarlet3

Use

Tags

6xHis

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV & Rha

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-C-mScarlet-Puro

Plasmid

#207722

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Puro cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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qTAG-C-mScarlet-Blast

Plasmid

#207711

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-2A-Blast cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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