We narrowed to 596 results for: pFAST

1. pFastBac1_GI.1_Q141V-P221L

   Plasmid

   #162584

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations Q141V-P221L in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Expression

   Insect

   Mutation

   Glutamine 141 changed to Valine and Proline 221 c…

   Promoter

   polyhedrin

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFastBac1_GI.1_L144C-P221C

   Plasmid

   #162585

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations L144C-P221C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Expression

   Insect

   Mutation

   Leucine 144 changed to Cysteine and Proline 221 c…

   Promoter

   polyhderin

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFastBac1_GI.1_G131C-N172C

   Plasmid

   #162586

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations G131C-N172C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Expression

   Insect

   Mutation

   Glycine 131 changed to Cysteine and Asparagine 17…

   Promoter

   polyhedrin

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFastBac1_GI.1_N167C-L169C

   Plasmid

   #162587

   Purpose

   Expresses norovirus GI.1 VP1 protein with mutations N167C-L169C in Sf9 insect cells

   Depositor

   Peter Kwong

   Article

   Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

   Insert

   VP1 protein from human norovirus GI.1
   

   Expression

   Insect

   Mutation

   Asparagine 167 changed to Cysteine and Leucine 16…

   Promoter

   polyhedrin

   Available Since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFast-dCry-V5His

   Plasmid

   #136240

   Purpose

   Protein expression of D. melanogaster (Dm) cytochrome with C-terminal V5 and His tags

   Depositor

   Aziz Sancar

   Article

   Ozturk et al Biochemistry. 2009 Sep 15;48(36):8585-93

   Insert

   DmCry
   

   Tags

   His and V5

   Expression

   Insect

   Available Since

   Jan. 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFastBac-GST-Mps1-KD

   Plasmid

   #114066

   Purpose

   protein production

   Depositor

   Geert Kops

   Article

   Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

   Insert

   Mps1-KD (TTK Human)
   

   Tags

   GST

   Expression

   Insect

   Mutation

   Mps1 KD (D664A)

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFastBac-GST-Mps1-WT

   Plasmid

   #114065

   Purpose

   protein production

   Depositor

   Geert Kops

   Article

   Jelluma et al PLoS One. 2008 Jun 11;3(6):e2415. doi: 1

   Insert

   Mps1-WT (TTK Human)
   

   Tags

   GST

   Expression

   Insect

   Mutation

   none

   Available Since

   Sept. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. Plekhm1 - C terminal pFastHFTb

   Plasmid

   #73829

   Purpose

   Express human Plekhm1 C-part in insect cells

   Depositor

   Paul Odgren

   Insert

   Plekhm1 (PLEKHM1 Human)
   

   Tags

   6xHis and Flag

   Expression

   Insect

   Mutation

   cloned C-terminal part of human Plekhm1 (aa 493- …

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

9. Plekhm1-N terminal pFastHFTb

   Plasmid

   #73828

   Purpose

   Express human Plekhm1 N-part in insect cells

   Depositor

   Paul Odgren

   Insert

   Plekhm1 (PLEKHM1 Human)
   

   Tags

   6xHis and Flag

   Expression

   Insect

   Mutation

   cloned N- terminal part of human Plekhm1 (aa 1- 4…

   Available Since

   March 15, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pFastBac-Gm129-Flag

    Plasmid

    #53766

    Purpose

    baculovirus expression of Gm129

    Depositor

    Aziz Sancar

    Article

    Annayev et al J Biol Chem. 2014 Feb 21;289(8):5013-24.

    Insert

    Gm129 (Ciart Mouse)
    

    Tags

    FLAG

    Expression

    Insect

    Promoter

    Polyhedrin

    Available Since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pFAST-BAC-Cdc20wt (2193)

    Plasmid

    #39850

    Depositor

    Jonathon Pines

    Article

    Nilsson et al Nat Cell Biol. 2008 Dec . 10(12):1411-20

    Insert

    Cdc20 (CDC20 Human)
    

    Use

    Baculovirus expression

    Tags

    His

    Available Since

    Oct. 31, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pFastBac-ATG101-ATG13 HORMA

    Plasmid

    #99332

    Purpose

    Mammalian expression of one subcomplex from the Human ULK1 complex

    Depositor

    James Hurley

    Article

    Qi et al Structure. 2015 Oct 6;23(10):1848-57. do

    Inserts

    ATG101 (ATG101 Human)
    ATG13 (ATG13 Human)
    

    Tags

    GST

    Expression

    Insect

    Promoter

    p10 and pH

    Available Since

    Aug. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFastBac Dual wt p110α/p85α

    Plasmid

    #199302

    Purpose

    co-expression of His-tagged p110α wt and Avi-tagged p85α wt proteins

    Depositor

    Peter Vogt

    Article

    Favalli et al Nat Chem. 2021 Jul;13(7):714. doi: 10.10

    Insert

    His-tagged p110α wt and Avi-tagged p85α wt (PIK3CA, PIK3R1 Human)
    

    Tags

    Avi and His

    Expression

    Insect

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFastBac Dual α1B/β3 Tubulin

    Plasmid

    #170552

    Purpose

    Encodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.

    Depositor

    Tarun Kapoor

    Article

    Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

    Inserts

    TUBA1B (TUBA1B Human)
    TUBB3 (TUBB3 Human)
    

    Tags

    AP linker, GS linker (GGSGG), His10 tag, L21 enha…

    Expression

    Insect

    Available Since

    June 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pFastBac_His-ZZ-TEV-Lis1-SNAPf

    Plasmid

    #133073

    Purpose

    Plasmid for insect cell expression of human full-length LIS1 with an N terminal His-ZZ-LTLT tag and C terminal SNAPf tag

    Depositor

    Andrew Carter, Simon Bullock

    Article

    Baumbach et al Elife. 2017 Apr 13;6. pii: e21768. doi:

    Insert

    Lis1 (PAFAH1B1 Human)
    

    Tags

    8x His, Linker-TEV-linker_TEV, SNAPf, and ZZ

    Expression

    Insect

    Available Since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFastBac1-OSF-TRIM5alpha(rhesus)

    Plasmid

    #79033

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    OSF-TRIM5alpha(rhesus)
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pFastBac1-Kozak-rhTRIM5alpha-PFOS

    Plasmid

    #79038

    Purpose

    Baculoviral entry vector to produce Bac-to-bac baculoviruses

    Depositor

    Wesley Sundquist

    Article

    Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

    Insert

    rhesus TRIM5alha-PFOS
    

    Tags

    OneSTrEP-FLAG

    Expression

    Insect

    Promoter

    polyhedrin

    Available Since

    Jan. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. 891 pFastBac GST PTEN

    Plasmid

    #10937

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Tags

    GST

    Expression

    Insect

    Available Since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

19. pFastBac-GST-DAXX-His

    Plasmid

    #175781

    Purpose

    DAXX protein for baculovirus expression system

    Depositor

    Xiaolu Yang

    Article

    Huang et al Nature. 2021 Aug 18. pii: 10.1038/s41586

    Insert

    death domain associated protein (DAXX Human)
    

    Tags

    GST and TEV

    Expression

    Insect

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFastBac_HT_B_6xHis-TEV-GFP-NBR1

    Plasmid

    #190928

    Purpose

    Plasmid for the expression and purification of GFP labelled NBR1. Internal reference: SMC 914

    Depositor

    Sascha Martens

    Article

    Turco et al Nat Commun. 2021 Sep 1;12(1):5212. doi:

    Insert

    NBR1 (NBR1 Human)
    

    Tags

    Strep-TEV-GFP

    Expression

    Insect

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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