Addgene

We narrowed to 305 results for: staphylococcus

Showing: 141 - 160 of 305 results

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pRN11

Plasmid

#84455

Purpose

shuttle plasmid for sarA P1-mCherry expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mCherry

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pCN-EF2132tet

Plasmid

#107191

Purpose

Expresses EF2132 recombinase for performing recombineering in S. aureus

Depositor

Steve Salipante

Article

Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

Inserts

EF2132
tetR

Use

Tags

Expression

Bacterial

Mutation

Promoter

p23

Available since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pTH3

Plasmid

#84453

Purpose

shuttle plasmid for sarA P1-YFP expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

YFP venus

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pUC18/promEco/Lysostaphin

Plasmid

#125765

Purpose

Production of staphylolytic protein lysostaphin from a constitutive promoter

Depositor

Benedykt Wladyka

Article

Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

Insert

lysostaphin

Use

Tags

Expression

Bacterial

Mutation

sequence corresponding to the mature form protein

Promoter

pemIK-Sa1 promoter

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pUC18/promSau/Lysostaphin

Plasmid

#125766

Purpose

Production of staphylolytic protein lysostaphin from a constitutive promoter

Depositor

Benedykt Wladyka

Article

Madry et al Mol Biotechnol. 2019 Oct;61(10):774-782.

Insert

lysostaphin

Use

Tags

Expression

Bacterial

Mutation

sequence corresponding to the mature form protein

Promoter

pemIK constitutive promoter

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pRN10

Plasmid

#84454

Purpose

shuttle plasmid for sarA P1-mKate expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mKate2

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pTH2

Plasmid

#84452

Purpose

shuttle plasmid for sarA P1-CFP expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

CFP Cerulean

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRN12

Plasmid

#84456

Purpose

shuttle plasmid for sarA P1-mAmetrine expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

mAmetrine

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pGBMCS-SortA

Plasmid

#21931

Depositor

Fuyuhiko Inagaki

Article

Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

Insert

sortase (srtA Staphylococcus aureus (strain N315))

Use

Tags

GB1 and His-tag

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only
pTH1

Plasmid

#84451

Purpose

shuttle plasmid for sarA P1-dsRED expression

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

DsRed.T3(DNT)

Use

Shuttle vector e.coli-s.aureus, expression of sgf…

Tags

Expression

Mutation

Promoter

Available since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pGBHPS-SortA

Plasmid

#21930

Depositor

Fuyuhiko Inagaki

Article

Kobashigawa et al J Biomol NMR. 2009 Mar . 43(3):145-50.

Insert

sortase

Use

Tags

GB1, HRV3C cleagage site, and His

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

Available since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0020

Plasmid

#117531

Purpose

Level0 Golden Gate part: CDS, SaCas9

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (with stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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Mammalian_SaAID

Plasmid

#188653

Purpose

Expresses SaAID in mammalian cells

Depositor

Keiji Nishida

Article

Li et al Nat Commun. 2022 Aug 8;13(1):4531. doi:

Insert

nSaCas9-AID-UGI

Use

Tags

UGI

Expression

Mammalian

Mutation

D10A for SaCas9

Promoter

pCMV

Available since

Nov. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD009

Plasmid

#116850

Purpose

Expresses araC constitutively and alpha-hemolysin under a pBAD promoter; alternate name pSB1A2-AD009

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
alpha-HL

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pSB1A3-AD010

Plasmid

#116851

Purpose

Expresses araC constitutively. Expresses alpha-hemolysin and mScarletI (RFP) under a pBAD promoter; alternate name pSB1A2-AD010

Depositor

Friedrich Simmel

Article

Dupin et al Nat Chem. 2018 Nov 26. pii: 10.1038/s415

Inserts

AraC
alpha-HL
mScarletI

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCF823_EFS-Cas9-PuroR_lenti

Plasmid

#138317

Purpose

Lenti expression of SpyCas9 with Puro selection

Depositor

Jennifer Doudna

Article

Watters et al Proc Natl Acad Sci U S A. 2020 Mar 10. p

Insert

SpyCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

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SAPDF-pET21a(+)

Plasmid

#12649

Depositor

SeWon Suh

Article

Yoon et al Proteins. 2004 Nov 15. 57(3):639-42.

Insert

SA Peptide deformylase

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0021

Plasmid

#117532

Purpose

Level0 Golden Gate part: CDS, SaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

SaCas9 (no stop codon)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pCN34e-yfp

Plasmid

#227641

Purpose

Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and erythromycin resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

yfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCN38-yfp

Plasmid

#227637

Purpose

Negative control plasmid encoding yfp downstream of an attenuated ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

yfp

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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