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pAAV_S34F_U2AF1

Plasmid

#176592

Purpose

Adenoviral construct to change S to F at 34th amino acid (S34F) in human U2AF1

Depositor

Manoj Pillai

Article

Boddu et al Commun Biol. 2021 Oct 13;4(1):1184. doi:

Insert

U2AF1 (U2AF1 Human)

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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NarCas9_AAV

Plasmid

#192145

Purpose

Expresses NarCas9?and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

Insert

NarCas9

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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AiP1343 - pAAV-AiE2051m-minBG-iCre

Plasmid

#220705

Purpose

AiE2051m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

iCre

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1344 - pAAV-AiE2052m-minBG-iCre

Plasmid

#220706

Purpose

AiE2052m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

iCre

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP1345 - pAAV-AiE2057m-minBG-iCre

Plasmid

#220707

Purpose

AiE2057m is an enhancer sequence, designed to induce cre-dependent recombination in specific populations of brain cells

Depositor

Bosiljka Tasic, The Allen Institute for Brain Science

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

iCre

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-AAV.MaCPNS2

Plasmid

#185137

Purpose

non-standard AAV2 rep-AAV-MaCPNS2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

Insert

Synthetic construct isolate AAV-MaCPNS2 VP1 gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

p41

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pUCmini-iCAP-AAV.MaCPNS1

Plasmid

#185136

Purpose

non-standard AAV2 rep-AAV-MaCPNS1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Article

Chen et al Neuron. 2022 Jul 20;110(14):2242-2257.e6

Insert

Synthetic construct isolate AAV-MaCPNS1 VP1 gene

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

p41

Available since

June 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV CMV GCGR-V5-sTurboID (N)

Plasmid

#153009

Purpose

Expresses split-TuboID N-terminal fragment fused to GCGR

Depositor

Alice Ting

Article

Cho et al Proc Natl Acad Sci U S A. 2020 May 18. p

Insert

GCGR-sTurboID (N) (L73)

Use

AAV

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mTagBFP2-WPRE

Plasmid

#193339

Purpose

AAV-mediated expression of mTagBFP2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mTagBFP2

Use

AAV

Tags

Expression

Mammalian

Mutation

A XhoI site in the coding sequence is silenced

Promoter

TRE

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMK231 (AAVS1 CMV-MCS-PURO)

Plasmid

#105924

Purpose

Donor for integration at the human AAVS1 locus

Depositor

Masato Kanemaki

Article

Okumura et al Elife. 2018 May 31;7. pii: 36559. doi: 1

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pEH_AAV_TERT_prom_-124C>T_C7C_1.8kb

Plasmid

#183080

Purpose

rAAV transfer plasmid with ITRs flanking: (1) TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

Depositor

Aviv Regev

Article

Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

Insert

TERT -124C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)

Use

AAV; Homologous recombination donor template

Tags

Expression

Mutation

-124C>T promoter, Exon 1 C7C (C21>T21) sile…

Promoter

None for primary insert (Though partial TERT prom…

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEH_AAV_TERT_prom_-146C>T_C7C_1.8kb

Plasmid

#183081

Purpose

rAAV transfer plasmid with ITRs flanking: (1) TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms, and (2) PGK-Puro.

Depositor

Aviv Regev

Article

Hodis et al Science. 2022 Apr 29;376(6592):eabi8175.

Insert

TERT -146C>T promoter and exon 1 C7C (C21>T21), 1.8kb homologous recombination donor template with ~900 bp homology arms (TERT Human)

Use

AAV; Homologous recombination donor template

Tags

Expression

Mutation

-146C>T promoter, Exon 1 C7C (C21>T21) sile…

Promoter

None for primary insert (Though partial TERT prom…

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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AAV2_HLF-ZE

Plasmid

#175034

Purpose

HLF targeting construct introducing an ires ZsG P2A tEGFR cassette downstream of the human HLF stop codon

Depositor

Guy Sauvageau

Article

Lehnertz et al Blood. 2021 Sep 9. pii: S0006-4971(21)01

Insert

HLF ires ZsGreen P2A tEGFR

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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AAV2_HLF-ZP

Plasmid

#175035

Purpose

HLF targeting construct introducing an ires ZsG P2A Puro cassette downstream of the human HLF stop codon

Depositor

Guy Sauvageau

Article

Lehnertz et al Blood. 2021 Sep 9. pii: S0006-4971(21)01

Insert

HLF ires ZsGreen P2A Puro

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

Oct. 26, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1α-DIO-CFL-SN

Plasmid

#181740

Purpose

AAV vector for expressing CFL-SN

Depositor

Yasunori Hayashi

Article

Goto et al Science. 2021 Nov 12;374(6569):857-863.

Insert

CFL-SN

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1alpha

Available since

April 6, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_CRF1.0

Plasmid

#208658

Purpose

Expresses the genetically-encoded fluorescent corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0 in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based corticotropin-releasing factor (CRF) sensor GRAB_CRF1.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_UCN1.0

Plasmid

#208669

Purpose

Expresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in a cre-dependent manner

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based urocortin (UCN) sensor GRAB_UCN1.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-miRFP

Plasmid

#108416

Purpose

Monomeric near-infrared fluorescent protein miRFP in mammalian-expressing AAV production plasmid

Depositor

Edward Boyden

Article

Piatkevich et al Nat Chem Biol. 2018 Feb 26. pii: 10.1038

Insert

miRFP

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_SST1.0

Plasmid

#208665

Purpose

Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based somatostatin (SST) sensor GRAB_SST1.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB_NPY1.0

Plasmid

#208676

Purpose

Expresses the genetically-encoded fluorescent neuropeptide Y (NPY) sensor GRAB_NPY1.0 in neurons

Depositor

Yulong Li

Article

Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

Insert

GPCR activation based neuropeptide Y (NPY) sensor GRAB_NPY1.0

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

Dec. 20, 2023

Availability

Academic Institutions and Nonprofits only

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