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Plasmid#128487PurposeAllelic exchange plasmid to to integrate firefly luciferase gene into S. pyogenes for biophotonic imagingDepositorInsertfirefly luciferase gene
UseAllelic replacement vectorTagsspy0535 downstream flanking region and spy0535 up…ExpressionMutationPromotersyntheticAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCVD402
Plasmid#66148Purposeto make a probe to detect Heat stable enterotoxin gene (human)DepositorInsertSTh
UseCloning vectorTagsExpressionMutationPromoternoneAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCVD404
Plasmid#66149Purposeto make a probe to detect Heat stable enterotoxin gene (porcine)DepositorInsertSTp
UseCloning vectorTagsExpressionMutationPromoternoneAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLIC-SM-Myc
Plasmid#111189PurposeHigh affinity Myc tagging of weakly expressed genes (optimized for Toxoplasma gondii)DepositorTypeEmpty backboneUseToxoplasma gondii expressionTagsSpaghetti Monster Myc TagExpressionMutationPromoterEndogenousAvailable sinceAug. 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC18/promEco/Lysostaphin
Plasmid#125765PurposeProduction of staphylolytic protein lysostaphin from a constitutive promoterDepositorInsertlysostaphin
UseTagsExpressionBacterialMutationsequence corresponding to the mature form proteinPromoterpemIK-Sa1 promoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLIC-SM-HA
Plasmid#111188PurposeHigh affinity HA Tagging of weakly expressed genes (optimized for Toxoplasma gondii)DepositorTypeEmpty backboneUseToxoplasma gondii expressionTagsSpaghetti Monster HA TagExpressionMutationPromoterEndogenousAvailable sinceAug. 2, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC18/promSau/Lysostaphin
Plasmid#125766PurposeProduction of staphylolytic protein lysostaphin from a constitutive promoterDepositorInsertlysostaphin
UseTagsExpressionBacterialMutationsequence corresponding to the mature form proteinPromoterpemIK constitutive promoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMK077: CaMKIIa QuasAr2-TS-GCaMP6f
Plasmid#72304PurposeCombines a gCaMP-based calcium indicator and an Arch-based voltage indicatorDepositorInsertCaViar
UseLentiviralTagsTSExpressionMammalianMutationPromoterCaMKIIaAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJB158
Plasmid#202601PurposeRP4-based mobilizable plasmid for the IPTG inducible expression of inserts that can be introduced using BsaI-based Golden Gate cloning. Vector has two origins of replication.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGCaMP5
Plasmid#78705PurposeGCaMP5 under the Toxoplasma SAG1 5' UTR. No Toxoplasma selectable markerDepositorInsertGCaMP5
UseTagsExpressionMutationPromoterSAG1 5' UTRAvailable sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac1-bullfrog saxiphilin
Plasmid#140595Purposeprotein expressionDepositorInsertsaxiphilin bullfrog
UseTagsGFP-His10ExpressionInsectMutationDeletion of M20 - please see depositor commentPromoterpolyhedrin promoterAvailable sinceNov. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMK074: CMV QuasAr2-TS-GCaMP6f
Plasmid#72303PurposeCombines a gCaMP-based calcium indicator and an Arch-based voltage indicatorDepositorInsertCaViar
UseLentiviralTagsTSExpressionMammalianMutationPromoterCMVAvailable sinceMay 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFCIV-CEACAM6
Plasmid#149303Purposelentiviral packaging vector for CEACAM6 expression.DepositorInsertCEACAM6 (CEACAM6 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterubiquitin CAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pU6-Decoy
Plasmid#80324PurposeEncodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in Toxoplasma gondiiDepositorInsertDecoy sgRNA
UseCRISPRTagsExpressionMutationPromoterU6Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSAG1::CAS9-U6::sg290860-6
Plasmid#59855PurposeEncodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.DepositorInsertCRISPR sg290860-6
UseCRISPR; ; toxoplasma gondiiTagsExpressionMutationPromoterAvailable sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-mTSP15
Plasmid#236542PurposeFor expression of Tspan15 in mammalian cellsDepositorInsertTSPAN15 (Tspan15 Mouse)
UseTagsGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-mTSP33
Plasmid#236541PurposeFor expression of Tspan33 in mammalian cellsDepositorInsertTSPAN33 (Tspan33 Mouse)
UseTagsGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
Pt NaV1.4
Plasmid#200233PurposeExpression in mammalian cells or oocytesDepositorInsertPhyllobates terriblis NaV1.4 – muscle channel
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
Dt NaV1.4
Plasmid#200250PurposeExpression in mammalian cells or oocytesDepositorInsertDendrobates tinctorius NaV1.4 – muscle channel
UseTagsExpressionMammalianMutationPromoterCMV and T7Available sinceSept. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pV5endoNA2
Plasmid#174729PurposeExpresses V5-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.DepositorInsertV5 epitope tag
UseTagsHistagExpressionBacterialMutationPromoterT5Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only