We narrowed to 851 results for: gfp cassette

1. pLib6.4-Cquin_801

   Plasmid

   #176657

   Purpose

   Expression of sgRNA under C. quinquefasciatus U6 (CPIJ039801) promoter & delivery of D.mel-Actin5C::EGFP-2A-puro|sgRNA cassette through PhiC31 RCME to AttP acceptor cell line/organism

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Type

   Empty backbone

   Use

   Crispr

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. AAT-PB-CG2APtk

   Plasmid

   #86003

   Purpose

   homology donor for AAT locus with piggyBac-flanked eGFP_T2A_puroTK cassette

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2016 Dec 2;6:38198. doi: 10.103

   Insert

   eGFP_T2A_puro_dTK
   

   Use

   Piggybac exciseable

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV-EF1A-LivePAR-Hygro

   Plasmid

   #176063

   Purpose

   EGFP fused to the C-terminus of a WWE domain & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   LivePAR
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1A

   Available since

   Jan. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pLV-U6-UL29-U3-UL8

   Plasmid

   #166685

   Purpose

   To express gRNA expression cassette simultaneously targeting two genes: UL8 and UL29 (non-EGFP version).

   Depositor

   Yujia Cai

   Article

   Yin et al Nat Biotechnol. 2021 Jan 11. pii: 10.103

   Insert

   UL8 and UL29 gRNA (UL29, UL8 HSV-1)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLV-EF1A-LivePAR(Y107A)-Hygro

   Plasmid

   #176073

   Purpose

   EGFP fused to the C-terminus of a WWE domain containing the mutation Tyr107Ala & a hygromycin resistance cassette

   Depositor

   Robert Sobol

   Article

   Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:

   Insert

   LivePAR (Y107A)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Point mutation to convert Try107 to Ala

   Promoter

   EF1A

   Available since

   Jan. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pXPR_071

   Plasmid

   #164558

   Purpose

   Higher titer version of pXPR_053. Lentiviral vector that enables constitutive sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

   Insert

   sgRNA cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHDR_G3BP1-V5-APEX2-mScarlet-I EF1a-Puro

   Plasmid

   #196196

   Purpose

   homologous recombination donor top insert V5-APEX2-mScarlet-I into the C-terminus of the human G3BP1 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   G3BP1 homology arms flanking V5-APEX2-mScarlet-I and EF1a-puro selection cassette (G3BP1 Human)
   

   Use

   Homology repair donor

   Tags

   V5-APEX2-mScarlet-I

   Expression

   Mutation

   Promoter

   Available since

   April 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pXPR_070

   Plasmid

   #164265

   Purpose

   Lentiviral vector that enables Cre-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

   Depositor

   Arlene Sharpe

   Article

   LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

   Insert

   sgRNA cassette
   

   Use

   CRISPR, Cre/Lox, and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Nov. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pPL001

   Plasmid

   #184750

   Purpose

   Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD.

   Depositor

   Jef Boeke

   Article

   Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

   Insert

   pEF1a-eGFP-T2A-BSD-bGHpA
   

   Use

   Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pXPR_068

    Plasmid

    #164555

    Purpose

    Lentiviral vector that enables Flp-mediated sgRNA expression. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.

    Depositor

    Arlene Sharpe

    Article

    LaFleur et al Nat Immunol. 2023 Nov 27. doi: 10.1038/s

    Insert

    sgRNA cassette
    

    Use

    CRISPR and Lentiviral; Flp/frt

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U6

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge