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pCellFree_G03 BTF3L4

Plasmid

#67051

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

BTF3L4 (BTF3L4 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
NET31 mRFP-N2 (490)

Plasmid

#61984

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Malik et al PLoS One. 2014 Nov 11;9(11):e111851. doi

Insert

NET31 (TMEM209 Human)

Use

Tags

mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pAN-OR-MalT-3NT

Plasmid

#62309

Purpose

synthetic circuit: OR gate, the sgRNA used for the NOT gate was substituted with one designed to target the malT gene in the E. coli genome

Depositor

Christopher Voigt

Article

Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

Inserts

A2NT sgRNA
Malt-3NT sgRNA
A2NT sgRNA

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

PA2, PPhlF, and pBAD

Available since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
tdEos-Cx43-7

Plasmid

#57603

Purpose

Localization: Gap Junctions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

Cx43

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pCMV-myc-hIFITM3-deltaY20

Plasmid

#58463

Purpose

Expresses human IFITM3-deltaY20 with an N-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

IFITM3 (IFITM3 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Tyrosine 20 deleted

Promoter

CMV IE

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-D92N

Plasmid

#58412

Purpose

Expresses murine IFITM3-D92N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Apartate 92 to Asparagine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K24,88,104A

Plasmid

#58406

Purpose

Expresses murine IFITM3-K24,88,104A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysines 24, 88, and 104 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-N2A

Plasmid

#58410

Purpose

Expresses murine IFITM3-N2A with an N-terminal HA tag in mammalian cells (mutates possible glycosylation site)

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Asparagine 2 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K24,83,104A

Plasmid

#58407

Purpose

Expresses murine IFITM3-K24,83,104A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysines 24, 83, and 104 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-S38N

Plasmid

#58411

Purpose

Expresses murine IFITM3-S38N with an N-terminal HA tag in mammalian cells (adds a possible glycosylation site)

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Serine 38 to Asparagine

Promoter

CMV IE

Available since

Sept. 5, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K24,83,88A

Plasmid

#58404

Purpose

Expresses murine IFITM3-K24,83,88A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysines 24, 83, and 88 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K88A

Plasmid

#58402

Purpose

Expresses murine IFITM3-K88A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysine 88 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-K104A

Plasmid

#58403

Purpose

Expresses murine IFITM3-K104A with an N-terminal HA tag in mammalian cells

Depositor

Howard Hang, Jacob Yount

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysine 104 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pET12a-AL-103 Y32F Y96F

Plasmid

#47092

Depositor

Marina Ramirez-Alvarado

Article

DiCostanzo et al J Biol Chem. 2012 Aug 10;287(33):27997-8

Insert

AL-103 Y32F Y96F (IGKV3-20 Human)

Use

Tags

ompT

Expression

Bacterial

Mutation

Y32F Y96F

Promoter

T7

Available since

Aug. 2, 2013

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-E222A-E272A

Plasmid

#35060

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Use

Tags

His

Expression

Mutation

Contains HinA domain: aa 192-393; E222A and E272A…

Promoter

T7

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-Y218A-Y267A

Plasmid

#35059

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Use

Tags

His

Expression

Mutation

Contains HinA domain: aa 192-393; Y218A and Y267A…

Promoter

T7

Available since

April 6, 2012

Availability

Academic Institutions and Nonprofits only
IFI16-(192-393)-HinA-E222A-E272A-E381A

Plasmid

#35061

Depositor

Cheryl Arrowsmith

Article

Liao et al Structure. 2011 Mar 9;19(3):418-29.

Insert

IFI16-(192-393)-HinA (IFI16 Human)

Use

Tags

His

Expression

Mutation

Contains HinA domain: aa 192-393; E222A, E272A, a…

Promoter

T7

Available since

April 4, 2012

Availability

Academic Institutions and Nonprofits only
pKD068

Plasmid

#136471

Purpose

SSB-GFP IDL fusion expression plasmid

Depositor

James Keck

Article

Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

Insert

SSB-GFP (ssb E. coli)

Use

Tags

superfolder GFP

Expression

Bacterial

Mutation

superfolder GFP inserted between Phe148 and Ser149

Promoter

T7 promoter

Available since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAIP hIL2 co

Plasmid

#90513

Purpose

Lentiviral vector for expression of human codon optimized IL2 with puromycin resistance.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

IL2 (IL2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEF-GFP

Plasmid

#11154

Purpose

Mammalian expression vector for expression of GFP (EF1a promoter)

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

EF1 alpha promoter (EEF1A1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1581 - 1600 of 4082 results

Results per page:

Sort results by:

pCellFree_G03 BTF3L4

NET31 mRFP-N2 (490)

pAN-OR-MalT-3NT

tdEos-Cx43-7

pCMV-myc-hIFITM3-deltaY20

pCMV-HA-mIFITM3-D92N

pCMV-HA-mIFITM3-K24,88,104A

pCMV-HA-mIFITM3-N2A

pCMV-HA-mIFITM3-K24,83,104A

pCMV-HA-mIFITM3-S38N

pCMV-HA-mIFITM3-K24,83,88A

pCMV-HA-mIFITM3-K88A

pCMV-HA-mIFITM3-K104A

pET12a-AL-103 Y32F Y96F

IFI16-(192-393)-HinA-E222A-E272A

IFI16-(192-393)-HinA-Y218A-Y267A

IFI16-(192-393)-HinA-E222A-E272A-E381A

pKD068

pAIP hIL2 co

pEF-GFP

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