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pMSCV-HA-human Cyclin L2 alpha-IRES-EYFP

Plasmid

#240142

Purpose

Express Cyclin L2 alpha

Depositor

Vincent Kidd, Jill Lahti, Martine Roussel

Article

Loyer et al J Biol Chem. 2008 Mar 21;283(12):7721-32

Insert

Cyclin L2 alpha (CCNL2 Human)

Use

Retroviral

Tags

HA

Promoter

MESVΨ

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-deltaExon15-III

Plasmid

#209567

Purpose

Mammalian expression of Shtn1 long isoform with E15 deletion III

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with E15 deletion III (4930506M07Rik Mouse)

Expression

Mammalian

Mutation

Exon15 deletion III

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

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An HA CAAX pMT2

Plasmid

#206116

Purpose

expression of Cav2.1 N-terminus (amino acids 1-100) with a C-terminal CAAX (last 10aa of H.Ras) motif and a HA tag

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

Ad MLP/TPL/SV40

Available Since

Nov. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGIG-FLAG-SHTN1LRRR>GGG

Plasmid

#209570

Purpose

Mammalian expression of Shtn1 long isoform with RG mutation

Depositor

Sika Zheng

Article

Zhang et al Neuron. 2019 Feb 20;101(4):690-706.e10.

Insert

Shtn1 long isoform with RG mutation (4930506M07Rik Mouse)

Expression

Mammalian

Mutation

RRR to GGG

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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An CAAX pMT2

Plasmid

#206118

Purpose

expression of Cav2.1 N-terminus (amino acids 1-100) with C-terminal CAAX (last 10 aa of H.Ras) motif

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

Ad MLP/TPL/SV40

Available Since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 D122A pcDNA3

Plasmid

#206068

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a D122A mutation that disrupts the interaction with ?2?-1

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-SHV_3CL

Plasmid

#203473

Purpose

Expresses SHV 3CL protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

SHV 3CL protease

Expression

Yeast

Promoter

GAL10

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-Bat-SL-CoVZC45_PLP

Plasmid

#201939

Purpose

Expresses Bat-SL-CoVZC45 papain-like protease from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

Bat-SL-CoVZC45 PLP

Expression

Yeast

Promoter

GAL1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-NWV_3CL

Plasmid

#203470

Purpose

Expresses NWV 3CL protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

NWV 3CL protease

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG426GAL-HCV_NS3-GS-NS4A

Plasmid

#203504

Purpose

Expresses HCV NS3-NS4A protease (with GS linker) from a GAL promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HCV NS3-GS-NS4A

Expression

Yeast

Promoter

GAL1

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-Bat-SL-CoVZC45_3CL

Plasmid

#201944

Purpose

Expresses Bat-SL-CoVZC45 3CL protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

Bat-SL-CoVZC45 3CL protease

Expression

Yeast

Promoter

GAL10

Available Since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL-3x-uC-HCoV-229E_3CL

Plasmid

#201937

Purpose

Expresses HCoV-229E 3CL protease from a GAL-3x promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HCoV-229E 3CL protease

Expression

Yeast

Promoter

GAL1

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAG416GAL10-HPeV_3C

Plasmid

#203465

Purpose

Expresses HPeV 3C protease from a GAL10 promoter with a URA3 marker

Depositor

Alejandro Chavez

Article

Hong et al Mol Syst Biol. 2025 Feb;21(2):158-172. d

Insert

HPeV 3C protease (polyprotein gene )

Expression

Yeast

Promoter

GAL10

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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pET21a Hp-iPGM-bio

Plasmid

#179615

Purpose

Expresses H. pylori iPGM containing a C-terminal biotinylation site and His tag in E. coli

Depositor

James Inglese

Article

van Neer et al ACS Chem Biol. 2022 Aug 19;17(8):2284-22

Insert

H. pylori iPGM bio-6His

Tags

His tag and biotinylation sequence

Expression

Bacterial

Promoter

T7

Available Since

Sept. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC-mTRF1deltaBLM

Plasmid

#64163

Purpose

Retroviral vector expressing mouse TRF1 lacking BLM-binding motifs with N-terminal Myc tag

Depositor

Titia de Lange

Article

Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

Insert

mTRF1 (Terf1 Mouse)

Use

Retroviral

Tags

Myc

Expression

Mammalian

Mutation

Deleted amino acids 313-316 and 339-342 (both BLM…

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-CHRFAM7ADelta2bp-YFP

Plasmid

#62637

Purpose

mammalian expression of human duplicated Alpha7-2 bp deletion (CHRFAM7A-∆2bp) with YFP fused into M3-M4 loop

Depositor

Henry Lester

Article

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRFAM7A (CHRFAM7A Human)

Expression

Mammalian

Mutation

there is a 2-bp deletion in exon 6 of CHRFAM7A an…

Promoter

CMV

Available Since

March 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV2.1-CMV-BD lacZ_3' Cer-bGHpA

Plasmid

#216319

Purpose

Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

Split Cerulean + splice acceptor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV2.1-CMV-5'Cer-BDlacZ-bGHpA

Plasmid

#216318

Purpose

Split fluorophore assay to test reconstitution via mRNA trans-splicing (REVeRT system).

Depositor

Elvir Becirovic

Article

Riedmayr et al Nat Commun. 2023 Oct 18;14(1):6578. doi:

Insert

split cerulean fluorescent protein + splice donor site

Use

AAV

Promoter

CMV

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57-mCherry-2A-BRD4 Iso C

Plasmid

#137721

Purpose

Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 short isoform

Depositor

Scott Floyd

Article

Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

Insert

BRD4 short isoform (BRD4 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

Tight TRE promoter

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLX317-SNAI1

Plasmid

#115446

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

SNAI1 (SNAI1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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