2,384 results
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Plasmid#184504PurposeMammalian expression of Synaptotagmin 3 with pH-sensitive fluorescent proteinDepositorAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only
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pZac2.1 gfaABC1D-BioID2-BioID2-Kir4.1-HA
Plasmid#176746PurposeExpresses Kir4.1 fused with BioID2 at the N-terminus in astrocytesDepositorInsertHA-BioID2-BioID2-Kir4.1 (Kcnj10 Aquifex aeolicus, Rat)
UseAAVTagsHAExpressionBacterialPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGADT7-αC
Plasmid#197650PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-2 αC fusion protein in yeast (yeast two-hybrid or yeast three-hybrid assays)DepositorInsertAP-2 αC
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationSer677T substitutionPromoterADH1Available sinceMarch 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
F3/pRC-CMV
Plasmid#197326PurposePlasmid expressing an antigen targeting the Contactin/F3 scaffold proteinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertContactin/F3 (Cntn1 Rat)
ExpressionMammalianAvailable sinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
GIT1-Flag/pBK
Plasmid#197313PurposePlasmid expressing an antigen targeting GIT1DepositorAvailable sinceMarch 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ3A
Plasmid#198174PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 μ3A fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 μ3A
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastMutationsilent substitution in codon 225PromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pACT2-μ3B
Plasmid#198175PurposeExpression of GAL4 transcriptional activation domain (AD)-AP-3 μ3B fusion protein in yeast (yeast two-hybrid assays)DepositorInsertAP-3 μ3B
TagsGAL4 transcriptional activation domain (AD) fragm…ExpressionYeastPromoterADH1Available sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLAMP1-miRFP718nano
Plasmid#195749PurposeLAMP1 (Lysosome Membrane) labelled with near-infrared fluorescent protein miRFP718nanoDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLAMP1-miRFP718nano
TagsmiRFP718nanoExpressionMammalianPromoterCMVAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
PH-miRFP-FRB
Plasmid#193568PurposeExpresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPH domain from PLC delta1 (Plcd1 Rat)
TagsFRB (T2098L) and miRFP703ExpressionMammalianMutationPH domainPromoterCMVAvailable sinceFeb. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pEGFP-C1-mScarlet-AMPKα2
Plasmid#192451PurposemScarlet-tagged alpha2 isoform of AMPK catalytic subunit.DepositorAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
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pLexm-gfp-Pept2
Plasmid#167988Purposeexpresses rat PepT2 with a c terminal cleavable GFP-His tag.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPepT2 (Slc15a2 Rat)
Tagscleavable GFP-his8ExpressionMammalianPromoterCMVAvailable sinceOct. 7, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
VAMP8
Plasmid#186689PurposeExpresses VAMP8 in E. ColiDepositorInsertVAMP8
Tags3xFLAG, GST, TEV, and ThrombinExpressionBacterialPromoterT7Available sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
Stx3
Plasmid#186685PurposeExpresses Syntaxin 3 in E. ColiDepositorInsertSyntaxin 3
Tags6 his tagExpressionBacterialAvailable sinceAug. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFUGW-shRIIα-RIIαWT-IRES-EGFP
Plasmid#183456PurposeReplacement of endogenous rat PKA-RIIα subunits with wild-type RIIαDepositorInsertsgRNA and Prkar2a (Prkar2a Rat)
UseLentiviralMutationshRNA-resistant mutations: C135>G, G138>A, …PromotershRNA: H1 / gene: ubiquitinAvailable sinceJune 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-AC8(1-106)
Plasmid#181958PurposeResidues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertEGFP-AC8(1-106) (Adcy8 Rat)
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
iLID-mCherry-iTrkA
Plasmid#136509PurposeMammalian expression of iLID fused to mCherry and intracellular signaling domain of TrkA (aa 450-799)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertiLID mCherry iTrkA (Ntrk1 Rat)
ExpressionMammalianPromoterCMVAvailable sinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSL007_PB_pCMV-H2B-mIFP-T2A-rTetR-ratKRAB-zeo
Plasmid#179438PurposeExpresses rTetR-KRAB(rat) for gene silencing, integration by PiggybacDepositorInsertH2B-mIFP-T2A-rTetR-KRAB
ExpressionMammalianPromoterpCMVAvailable sinceMay 24, 2022AvailabilityAcademic Institutions and Nonprofits only