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pMT2 Alpha2 delta-1 V6

Plasmid

#89894

Purpose

Mammalian expression of Alpha2 delta-1 V6

Depositor

Annette Dolphin

Article

Kadurin et al Elife. 2016 Oct 26;5. pii: e21143. doi:

Insert

Cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Mutation

Amino acids L943V, E944V, A945V, E947V, M948V are…

Promoter

adenovirus major late promoter

Available Since

April 25, 2017

Availability

Academic Institutions and Nonprofits only

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Myc-PCDHGC5

Plasmid

#119935

Purpose

Protocadherin gammaC5 expression (Myc-tag is at the linker between EC1 and EC2 extracellular cadherin repeats)

Depositor

Angel de Blas

Article

Li et al J Neurosci. 2012 Aug 22;32(34):11780-97.

Insert

PCDHGC5 (Pcdhgc5 Rat)

Tags

9E10 cMyc epitope (EQKLISEEDL) was inserted betwe…

Expression

Mammalian

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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mRuby-Cx43-7

Plasmid

#55856

Purpose

Localization: Gap Junctions, Excitation: 558, Emission: 605

Depositor

Michael Davidson

Insert

Cx43

Tags

mRuby

Expression

Mammalian

Promoter

CMV

Available Since

March 12, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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pBS rat IRS-1

Plasmid

#11027

Depositor

Ronald Kahn

Insert

IRS-1 (Irs1 Rat)

Expression

Bacterial

Available Since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
pPRIME-CMV-GFP-shDyn1

Plasmid

#132711

Purpose

Encodes short hairpin RNA (shRNA) #1 that targets the 3’-untranslated region of the rat Pdyn gene

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shDyn(1) (Pdyn Rat)

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pCNL-N1_ITPKA

Plasmid

#65688

Purpose

Expresses ITPKA-CNL in mammalian cells

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

inositol triphosphate 3-kinase A

Use

Luciferase

Tags

CNL

Expression

Mammalian

Mutation

contains residues 9-52

Promoter

CMV

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Cacnb1 KI

Plasmid

#139660

Purpose

Endogenous tagging of CaVβ1: N-terminal (amino acid position: S8)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE Cacnb2-GFP KI

Plasmid

#139661

Purpose

Endogenous tagging of CaVβ2: C-terminal (amino acid position: Q655)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pORANGE C9orf4-GFP KI

Plasmid

#131472

Purpose

Endogenous tagging of C9orf4: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Frrs1l Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-CaMKKbeta rat S100A, S495A and S511A

Plasmid

#33320

Depositor

Anthony Means

Article

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S99A, S494A, S510A

Promoter

SV40

Available Since

April 23, 2012

Availability

Academic Institutions and Nonprofits only
pFSW Syt1-C2A IRES GFP

Plasmid

#133818

Purpose

Encodes the C2A domain of synaptotagmin 1 for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Promoter

hSyn (human synapsin I promoter)

Available Since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

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Citrine-CathespinB-6

Plasmid

#56554

Purpose

Localization: Lysosomes, Excitation: 516, Emission: 529

Depositor

Michael Davidson

Insert

CathespinB

Tags

Citrine

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pPRIME-CMV-GFP-shCrh1

Plasmid

#132709

Purpose

Encodes short hairpin RNA (shRNA) #1 that targets the 3’-untranslated region of the rat Crh gene

Depositor

Robert Messing

Article

Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

Insert

shCrh(1) (Crh Rat)

Use

Lentiviral

Promoter

CMV

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pJC20-Tpm4.2 (Rn)

Plasmid

#99359

Purpose

Bacterial expression vector containing cDNA encoding for the Rattus norvegicus tropomyosin, Tpm4.2 under the control of the pRhamnose promoter

Depositor

Dan Mulvihill

Article

Eastwood et al FEBS Lett. 2017 Feb 18. doi: 10.1002/187

Insert

Tpm4.2 (Tpm4 Rat)

Expression

Bacterial

Promoter

Rhamnose

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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Septin 11 isoform II

Plasmid

#120315

Purpose

Septin 11 isoform II mammalian expression

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pGEM rat islet1 (phoII) [TJ#1]

Plasmid

#16268

Depositor

Thomas Jessell

Insert

islet1 (Isl1 Rat)

Expression

Bacterial

Available Since

Feb. 4, 2009

Availability

Academic Institutions and Nonprofits only
mTFP1-Cx26-7

Plasmid

#55473

Purpose

Localization: Gap Junctions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Cx26

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
pRK5-TGF beta type I receptor (mL45TD)

Plasmid

#34702

Depositor

Ying Zhang

Article

Yu et al EMBO J. 2002 Jul 15;21(14):3749-59.

Insert

TGF beta type I receptor (Tgfbr1 Rat)

Tags

Flag

Expression

Mammalian

Mutation

T202D, N265A, D267A, N268A

Promoter

CMV

Available Since

June 22, 2012

Availability

Academic Institutions and Nonprofits only
pGL2-HKII-D

Plasmid

#64516

Purpose

AS-30D hepatoma hexokinase II 1.0 kbp promoter-luciferase reporter vector

Depositor

Peter Pedersen

Article

Mathupala et al J Biol Chem. 2001 Nov 16;276(46):43407-1

Insert

Type II hexokinase gene, partial cds and promoter region (Hk2 Rat)

Use

Luciferase

Tags

firefly luciferase

Mutation

Isolated from AS-30D rat hepatoma

Promoter

Hexokinase II promoter

Available Since

June 23, 2015

Availability

Academic Institutions and Nonprofits only
mCit-Snap25b D179K A199R

Plasmid

#53259

Purpose

SNAP25B double mutant insensitive to both Botulinum toxin C1 & E; N-terminal mCitrine tag connected by 4 amino acid linker. For mammalian cells, used with mCer-4aa-VAMP2 to measure FRET & dispersion.

Depositor

Robert Zucker

Article

Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

Insert

SNAP-25B (Snap25 Rat)

Tags

4aa-linker and mCitrine

Expression

Mammalian

Mutation

D179K and A199R

Promoter

CMV

Available Since

April 21, 2015

Availability

Academic Institutions and Nonprofits only