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Plasmid#121783PurposepMVP L3-L2 entry plasmid, contains Zeo-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Zeo selection marker linked by P2A to gene of interest.DepositorInsertP2A-Zeo + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KZ901: pMVP (L3-L2) P2A-eGFP + WPRE
Plasmid#121772PurposepMVP L3-L2 entry plasmid, contains eGFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest in lentivirus vectors.DepositorInsertP2A-eGFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KO401: pMAGIC (R4-R3) NLS-x Cas9(3.7)-NLS
Plasmid#121834PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS xCas9(3.7) (nuclease) fusion for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-Cas9(3.7) (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KZ001: pMVP (L3-L2) P2A-eYFP + polyA
Plasmid#121770PurposepMVP L3-L2 entry plasmid, contains eYFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eYFP linked by P2A to gene of interest.DepositorInsertP2A-eYFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LA601: pMVP (L3-L2) P2A-Puro + WPRE
Plasmid#121785PurposepMVP L3-L2 entry plasmid, contains Puro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to a gene in lentivirus vectorsDepositorInsertP2A-Puro + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KZ501: pMVP (L3-L2) P2A-Puro + polyA
Plasmid#121780PurposepMVP L3-L2 entry plasmid, contains Puro-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Puro selection marker linked by P2A to gene of interest.DepositorInsertP2A-Puro + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KY901: pMVP (L3-L2) P2A-mCherry + polyA
Plasmid#121766PurposepMVP L3-L2 entry plasmid, contains mCherry-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest.DepositorInsertP2A-mCherry + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HD201: pMVP (L3-L2) IRES-eGFP + polyA
Plasmid#121747PurposepMVP L3-L2 entry plasmid, contains IRES2-eGFP+ polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of eGFP reporter from IRES sequence.DepositorInsertIRES2-eGFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LA001: pMVP (L3-L2) P2A-mCherry + WPRE
Plasmid#121774PurposepMVP L3-L2 entry plasmid, contains mCherry-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term mCherry linked by P2A to gene of interest in lentivirus vectors.DepositorInsertP2A-mCherry + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IA201: pMAGIC (R4-R3) NLS-Sa dCas9-NLS
Plasmid#121821PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertSa-dCas9 (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA501: pMVP (L3-L2) pA; CMV::TETa-pA
Plasmid#121801PurposepMVP L3-L2 entry plasmid, contains polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds CMV-driven TETa downstream of gene of interest.DepositorInsertpolyA + CMV::TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LA201: pMVP (L3-L2) P2A-eCFP + WPRE
Plasmid#121776PurposepMVP L3-L2 entry plasmid, contains eCFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eCFP linked by P2A to gene of interest.DepositorInsertP2A-eCFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBCMV-MS2CP-eDHFR
Plasmid#167309PurposeTemplate pDNA to prepare a split CaVT component mRNADepositorInsertMS2CP-eDHFR
UseSynthetic BiologyTagsDYK-tagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
KN701: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS
Plasmid#121828PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) (nuclease-dead) fusion for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertx-dCas9(3.7) (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KO701: pMVP (L3-L2) HA tag + pA; EF1a::TETa-pA
Plasmid#121806PurposepMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven TETaDepositorInsertHA epitope tag-polyA + EF1a::TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KH001: pMVP (L3-L2) V5 epitope tag + WPRE
Plasmid#121762PurposepMVP L3-L2 entry plasmid, contains V5 epitope tag + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows C-term epitope fusion to a gene of interest in lentivirus vectors.DepositorInsertV5 epitope tag + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KZ701: pMVP (L3-L2) AID + polyA
Plasmid#121791PurposepMVP L3-L2 entry plasmid, contains AID + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term Auxin Induced Degron (AID) domain (activated by auxin) to geneDepositorInsertAID + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KY801: pMVP (L3-L2) P2A-eGFP + polyA
Plasmid#121764PurposepMVP L3-L2 entry plasmid, contains eGFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eGFP linked by P2A to gene of interest.DepositorInsertP2A-eGFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LA102: pMVP (L3-L2) P2A-eYFP + WPRE
Plasmid#121778PurposepMVP L3-L2 entry plasmid, contains eYFP-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eYFP linked by P2A to gene of interest in lentivirus vectors.DepositorInsertP2A-eYFP + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LA301: pMVP (L3-L2) P2A-Hygro + WPRE
Plasmid#121787PurposepMVP L3-L2 entry plasmid, contains Hygro-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Hygro selection marker linked by P2A to gene in lentivirus vectorsDepositorInsertP2A-Hygro + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LA802: pMVP (L3-L2) AID + WPRE
Plasmid#121794PurposepMVP L3-L2 entry plasmid, contains AID + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows fusion of minimal C-term AID domain (activated by auxin) to gene in lentivirus vectorsDepositorInsertAID + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KO801: pMVP (L5-L4) Hygro-P2A
Plasmid#121718PurposepMVP L5-L4 entry plasmid, contains Hygro-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term Hygro selection marker linked by P2A to gene of interest.DepositorInsertHygro-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscAAV.CAG-EGFP-SV40pA-T7-T3-BC(p1-14)
Plasmid#231346PurposeSelf complementary AAV genome with components for tracking self-complementary AAV genomes via AAV-Zombie. Also expresses EGFP from CAG promoter.DepositorInsertT7/T3-BC(p1-14)
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCas3-Apr
Plasmid#208000PurposeExpresses all necessary components of Type I-C CRISPR-Cas systemDepositorUseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRa_all-in-one_Bip gRNA1
Plasmid#183696PurposeExpresses gRNA targeting chinise hamster Bip (Hspa5) with CRISPRa components and mCherry, blasticidin selectiveDepositorInsertgRNA targeting chinese hamster Bip (Hspa5)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCas9FnCpf1GG
Plasmid#131013PurposeBackbone plasmid for generating CRISPR arrays for composite array utilized by SpCas9 and FnCas12a using CRATES. Contains a GFP-dropout cassette and two direct repeats.DepositorInsertsdirect repeat of SpCas9
GFP expression cassette
direct repeat of FnCas12a
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
IF405: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-p300core
Plasmid#121825PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the p300 catalytic core for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertSa-dCas9/p300core (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IP803: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-Dnmt3a3L
Plasmid#121826PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertSa-dCas9/Dnmt3a-3L (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KO602: pMVP (L3-L2) HA tag + pA; CMV::TETa-pA
Plasmid#121802PurposepMVP L3-L2 entry plasmid, contains HA tag-polyA + CMV-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream CMV-driven TETaDepositorInsertHA epitope tag-polyA + CMV::TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA601: pMVP (L3-L2) pA; EF1a::TETa-pA
Plasmid#121805PurposepMVP L3-L2 entry plasmid, contains polyA + EF1a-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds EF1a-driven TETa downstream of gene of interest.DepositorInsertpolyA + EF1a::TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA401: pMVP (L3-L2) pA; RIP::TETa-pA
Plasmid#121809PurposepMVP L3-L2 entry plasmid, contains polyA + RIP-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds RIP-driven TETa downstream of gene of interest.DepositorInsertpolyA + RIP::TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KO502: pMVP (L3-L2) HA tag + pA; RIP::TETa-pA
Plasmid#121810PurposepMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven TETaDepositorInsertHA epitope tag-polyA + RIP::TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IA304: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-VPR
Plasmid#121822PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the VPR transcriptional activator for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertSa-dCas9/VPR (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IB801: pMAGIC (R4-R3) NLS-Sa dCas9-NLS-KRAB
Plasmid#121823PurposepMAGIC R4-R3 entry plasmid, contains 2x NLS Sa-dCas9 fused to the KRAB transcriptional repressor for 3 or 4-component MultiSite Gateway Pro assembly.DepositorInsertSa-dCas9/KRAB (open)
UseSynthetic Biology; Pmagic gateway entry plasmidTagsNLSExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KZ101: pMVP (L3-L2) P2A-eCFP + polyA
Plasmid#121768PurposepMVP L3-L2 entry plasmid, contains eCFP-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term eCFP linked by P2A to gene of interest.DepositorInsertP2A-eCFP + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KZ301: pMVP (L3-L2) P2A-Blast + polyA
Plasmid#121779PurposepMVP L3-L2 entry plasmid, contains Blast-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Blast selection marker linked by P2A to gene of interest.DepositorInsertP2A-Blast + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KZ201: pMVP (L3-L2) P2A-Hygro + polyA
Plasmid#121782PurposepMVP L3-L2 entry plasmid, contains Hygro-P2A + polyA for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Hygro selection marker linked by P2A to gene of interest.DepositorInsertP2A-Hygro + polyA
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LA401: pMVP (L3-L2) P2A-Blast + WPRE
Plasmid#121784PurposepMVP L3-L2 entry plasmid, contains Blast-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Blast selection marker linked by P2A to gene in lentivirus vectorsDepositorInsertP2A-Blast + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LA501: pMVP (L3-L2) P2A-Neo + WPRE
Plasmid#121786PurposepMVP L3-L2 entry plasmid, contains Neo-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Neo selection marker linked by P2A to a gene in lentivirus vectorsDepositorInsertP2A-Neo + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
LA701: pMVP (L3-L2) P2A-Zeo + WPRE
Plasmid#121788PurposepMVP L3-L2 entry plasmid, contains Zeo-P2A + WPRE for 3- or 4-component MultiSite Gateway Pro assembly. Allows expression of C-term Zeo selection marker linked by P2A to a gene in lentivirus vectors.DepositorInsertP2A-Zeo + WPRE
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only