Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 6,740 results for: tac

Showing: 3951 - 4000 of 6740 results

  1. LT3GEPIR-NEK9shRNA2

    Plasmid
    #220555
    Purpose
    To inducibly knockdown NEK9 expression
    Depositor
    Sean Lee
    Insert
    NEK9 shRNA2 (NEK9 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    TRE3G (TetOP) promoter
    Available since
    Nov. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    220555-plasmid-map-sequence-id-453888

    Enlarge

  2. pCfb13210

    Plasmid
    #219885
    Purpose
    The base plasmid of TUNEYALI for TF20
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF20 (YALI1_D16173g) and homologous arm matching TF20
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Nov. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219885-plasmid-map-sequence-id-435623

    Enlarge

  3. pCfb13204

    Plasmid
    #219879
    Purpose
    The base plasmid of TUNEYALI for TF14
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF14 (YALI1_F21261g) and homologous arm matching TF14
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    219879-plasmid-map-sequence-id-436035

    Enlarge

  4. pCfb13200

    Plasmid
    #219875
    Purpose
    The base plasmid of TUNEYALI forTF10
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF10 ( YALI1_C16204g) and homologous arm matching TF10
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    219875-plasmid-map-sequence-id-435956

    Enlarge

  5. pCfb13193

    Plasmid
    #219868
    Purpose
    The base plasmid of TUNEYALI for TF03
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF03 (YALI1_B05350g) and homologous arm matching TF03
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    219868-plasmid-map-sequence-id-435948

    Enlarge

  6. pCfb13225

    Plasmid
    #219900
    Purpose
    The base plasmid of TUNEYALI for TF35
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF35 (YALI1_E34586g) and homologous arm matching TF35
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    219900-plasmid-map-sequence-id-435505

    Enlarge

  7. pCfb13203

    Plasmid
    #219878
    Purpose
    The base plasmid of TUNEYALI forTF13
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF13 (YALI1_F21261g) and homologous arm matching TF13
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    219878-plasmid-map-sequence-id-435471

    Enlarge

  8. pCfb13224

    Plasmid
    #219899
    Purpose
    The base plasmid of TUNEYALI for TF34
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF34 (YALI1_E37455g) and homologous arm matching TF34
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219899-plasmid-map-sequence-id-435425

    Enlarge

  9. pCfb13205

    Plasmid
    #219880
    Purpose
    The base plasmid of TUNEYALI for TF15
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF15 (YALI1_B29562g) and homologous arm matching TF15
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219880-plasmid-map-sequence-id-435423

    Enlarge

  10. pCfb13235

    Plasmid
    #219908
    Purpose
    The base plasmid of TUNEYALI for TF45
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF45 ( YALI1_B23373g) and homologous arm matching TF45
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219908-plasmid-map-sequence-id-435389

    Enlarge

  11. pCfb13248

    Plasmid
    #219919
    Purpose
    The base plasmid of TUNEYALI for TF58
    Depositor
    Irina Borodina
    Insert
    Contains gRNA targeting TF58 (YALI1_F37742g) and homologous arm matching TF58
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    June 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    219919-plasmid-map-sequence-id-435379

    Enlarge

  12. SDCBPA-c012

    Plasmid
    #205120
    Purpose
    SDCBP protein expression
    Depositor
    Paul Brennan
    Insert
    SDCBP (SDCBP Human)
    Use
    Tags
    6His-TEV and AviTag
    Expression
    Bacterial
    Mutation
    WT
    Promoter
    Available since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    205120-plasmid-map-sequence-id-411022

    Enlarge

  13. pT2/shαSMA/GFP4_Seq2/clone1

    Plasmid
    #201402
    Purpose
    Knockdown of alpha-SMA. Construct has inverted repeats to be used in Sleeping Beauty transposon system.
    Depositor
    Maria Castro
    Insert
    alpha-SMA
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    201402-plasmid-map-sequence-id-394260

    Enlarge

  14. SGEP-sh-Hs-PHGDH-1968

    Plasmid
    #188673
    Purpose
    shRNA
    Depositor
    Lewis Cantley
    Insert
    shPHGDH (PHGDH Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188673-plasmid-map-sequence-id-375111

    Enlarge

  15. mU6-sgSik1_2nd-hU6-sgNT

    Plasmid
    #177213
    Purpose
    Expresses a Sik1-targeting (mU6) and a non-targeting (hU6) gRNAs and Cre recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_2nd/sgNT1
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    mU6/hU6
    Available since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177213-plasmid-map-sequence-id-349895

    Enlarge

  16. pPHTF2.1.0-gDNA

    Plasmid
    #132465
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    PHTF2 (PHTF2 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    132465-plasmid-map-sequence-id-267085

    Enlarge

  17. pZNF253.1.0-gDNA

    Plasmid
    #132461
    Purpose
    CRISPR/Cas9 plasmid to create GFP fusion proteins
    Depositor
    Kevin White
    Insert
    ZNF253 (ZNF253 Human)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 19, 2019
    Availability
    Academic Institutions and Nonprofits only
    132461-plasmid-map-sequence-id-267028

    Enlarge

  18. T2m

    Plasmid
    #121029
    Purpose
    MoClo golden gate assembly DE part for Eck120033736 (164.6x-strength natural terminator; see DOI: 10.1038/nmeth.2515) - Please see Supplemental Documents for annotated Genbank file
    Depositor
    Richard Murray
    Insert
    ECK120033736 (164.6x Voigt terminator), short attachment
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    121029-plasmid-map-sequence-id-238076

    Enlarge

  19. SPLICSFAST-LDh-MT-P2A

    Plasmid
    #214896
    Purpose
    Expresses equimolar Lipid Droplet- and Mitochondria- targeted split-FAST fragments for Short-range LD-MT Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-LDh-MT-P2A
    Use
    Tags
    none
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214896-plasmid-map-sequence-id-423482

    Enlarge

  20. SPLICSFAST-LDh-LY-P2A

    Plasmid
    #214895
    Purpose
    Expresses equimolar Lipid Droplet- and Lysosome- targeted split-FAST fragments for Short-range LD-LY Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-LDh-LY-P2A
    Use
    Tags
    none
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214895-plasmid-map-sequence-id-423481

    Enlarge

  21. SPLICSFAST-ER-MT-P2A

    Plasmid
    #214892
    Purpose
    Expresses equimolar Outer mitochondrial membrane- and Endoplasmic Reticulum- targeted split-FAST fragments for Short-range ER-Mito Contact sites
    Depositor
    Tito Calì
    Insert
    SPLICSFAST-ER-MT-P2A
    Use
    Tags
    none
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    214892-plasmid-map-sequence-id-423478

    Enlarge

  22. pHTganA-Cpf1

    Plasmid
    #158647
    Purpose
    Constitutive transcription of FnCpf1 and ganA crRNA
    Depositor
    Wenjing Cui
    Insert
    Cpf1, ganA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    P43
    Available since
    Oct. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    158647-plasmid-map-sequence-id-314144

    Enlarge

  23. pADsacA

    Plasmid
    #158649
    Purpose
    Constitutive transcription of sacA crRNA and GFP
    Depositor
    Wenjing Cui
    Insert
    sacA crRNA
    Use
    CRISPR and Synthetic Biology; Shuttle vector baci…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Pveg
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    158649-plasmid-map-sequence-id-310569

    Enlarge

  24. pX601-miniCMV-SaCas9-U6-LacZvsSaCas9 sgRNA

    Plasmid
    #107049
    Purpose
    All-in-one vectors expressing both SaCas9 and LacZ sgRNA
    Depositor
    Alex Hewitt
    Insert
    SaCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107049-plasmid-map-sequence-id-208585

    Enlarge

  25. pX601-miniCMV-SaCas9-U6-YFPvsSaCas9 sgRNA

    Plasmid
    #107050
    Purpose
    All-in-one vectors expressing both SaCas9 and YFP sgRNA
    Depositor
    Alex Hewitt
    Insert
    SaCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107050-plasmid-map-sequence-id-208586

    Enlarge

  26. pENTRY4_ Pi21

    Plasmid
    #126904
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126904-plasmid-map-sequence-id-249827

    Enlarge

  27. pHnS KI;Gria1 Donor;3xV5 KO;Template

    Plasmid
    #240293
    Purpose
    KI:Gria1 Donor:3xV5 KO:Template
    Depositor
    Yuki Ogawa
    Insert
    KI gRNA for Gria1
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    240293-plasmid-map-sequence-id-478520

    Enlarge

  28. pHnS 2comp Donor;smFP-V5 KO;Gphn#2

    Plasmid
    #240308
    Purpose
    Donor:smFP-V5 KO:Gphn#2
    Depositor
    Yuki Ogawa
    Insert
    KO gRNAs for Gphn
    Use
    AAV
    Tags
    Expression
    Mutation
    NA
    Promoter
    Available since
    July 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    240308-plasmid-map-sequence-id-478515

    Enlarge

  29. pICSL4723_ Pi21

    Plasmid
    #126892
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126892-plasmid-map-sequence-id-249677

    Enlarge

  30. pICSL4723_ HDT701

    Plasmid
    #126886
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126886-plasmid-map-sequence-id-249696

    Enlarge

  31. pICSL4723_ Rac5

    Plasmid
    #126885
    Purpose
    Genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 module, nptII resistance gene, and specific sgRNA modules on the binary plasmid pICSL4723.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    126885-plasmid-map-sequence-id-249710

    Enlarge

  32. pENTRY4_Rac5

    Plasmid
    #126897
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    126897-plasmid-map-sequence-id-250096

    Enlarge

  33. pENTRY4_ HDT701

    Plasmid
    #126898
    Purpose
    Gateway pENTR-plasmid to generate a binary construct for genome editing of the wheat homolog of a susceptibility gene of O. sativa. Encodes wheat optimized Cas9 and gene specific sgRNA modules.
    Depositor
    Sophien Kamoun
    Insert
    Wheat_live_Cas9
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    126898-plasmid-map-sequence-id-249718

    Enlarge

  34. CFP(159-238)Stop in pcDNA1/amp

    Plasmid
    #60893
    Purpose
    This vector attached CFP(159-238) to the C-terminus of a protein.
    Depositor
    Catherine Berlot
    Insert
    CFP(159-238)Stop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn-164 was changed to His
    Promoter
    CMV
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    60893-plasmid-map-sequence-id-118771

    Enlarge

  35. pNSU299_GAL2

    Plasmid
    #166088
    Purpose
    Plasmid for constituive spCas9 and tet-inducible GAL2 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    GAL2 (GAL2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166088-plasmid-map-sequence-id-329112

    Enlarge

  42. ipUSEPR-sg-Hs-TMPRSS2-A6

    Plasmid
    #188690
    Purpose
    sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgTMPRSS2 (TMPRSS2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188690-plasmid-map-sequence-id-375121

    Enlarge

  43. bU6-sgSik1_1st-mU6-sgSik2-hU6-sgSik3_1st

    Plasmid
    #177232
    Purpose
    Expresses Sik1(bU6), Sik2 (mU6) and Sik3 (hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgSik1_1st/sgSik2/sgSik3_1st
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    June 9, 2022
    Availability
    Academic Institutions and Nonprofits only
    177232-plasmid-map-sequence-id-363978

    Enlarge

  44. bU6-sgNeo1-mU6-sgNuak1-hU6-sgNuak2

    Plasmid
    #177234
    Purpose
    Expresses Neomycin (bU6), Nuak1 (mU6) and Nuak2(hU6) gRNAs and Cre-recombinase
    Depositor
    Monte Winslow
    Insert
    sgNeo1/sgNuak1/sgNuak2
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    bU6/mU6/hU6
    Available since
    Jan. 24, 2022
    Availability
    Academic Institutions and Nonprofits only
    177234-plasmid-map-sequence-id-352695

    Enlarge

  46. Scramble_gRNA_puro

    Plasmid
    #234999
    Purpose
    scramble sgRNA
    Depositor
    Samrat Roy Choudhury
    Insert
    scramble sgRNA
    Use
    Lentiviral
    Tags
    EGFP:T2A:Puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    234999-plasmid-map-sequence-id-477921

    Enlarge

  47. pML107-RER2

    Plasmid
    #232884
    Purpose
    Plasmid expressing Cas9 and gRNA AGAATCGCATCTCTACACGG which targets the RER2 gene.
    Depositor
    Jolanda Van Leeuwen
    Insert
    RER2 gRNA (RER2 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    snR52
    Available since
    Feb. 26, 2025
    Availability
    Academic Institutions and Nonprofits only
    232884-plasmid-map-sequence-id-462289

    Enlarge

  49. ipUSEPR-sg-Hs-TMPRSS2-A5

    Plasmid
    #188689
    Purpose
    sgRNA
    Depositor
    Lewis Cantley
    Insert
    sgTMPRSS2 (TMPRSS2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mutation
    WT
    Promoter
    Available since
    Oct. 21, 2022
    Availability
    Academic Institutions and Nonprofits only
    188689-plasmid-map-sequence-id-375120

    Enlarge

  50. pNSU299_CPR1_1

    Plasmid
    #166074
    Purpose
    Plasmid for constituive spCas9 and tet-inducible CPR1 targeting sgRNA expression for double stranded break formation in yeast
    Depositor
    Rebecca Burgess
    Insert
    CPR1 (CPR1 Budding Yeast)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Tet-inducible
    Available since
    April 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    166074-plasmid-map-sequence-id-330357

    Enlarge

Showing: 3951 - 4000 of 6740 results