We narrowed to 15,024 results for: pET

1. YjdC-R82A_pET-14b

   Plasmid

   #233276

   Purpose

   Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 82 was substituted with alanine (R82A)

   Depositor

   Andrew Emili, Mohan Babu

   Article

   Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

   Insert

   YjdC (yjdC )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Arginine was replaced with Alanine at position 82…

   Promoter

   T7 promoter

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. YjdC-R64A_pET-14b

   Plasmid

   #233263

   Purpose

   Overexpress his-tag YjdC protein with a site directed mutagenesis, arginine at position 64 was substituted with alanine (R64A)

   Depositor

   Andrew Emili, Mohan Babu

   Article

   Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

   Insert

   YjdC (yjdC )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Arginine was replaced with Alanine at position 64…

   Promoter

   T7 promoter

   Available Since

   March 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pETG20a-Salro-HMG

   Plasmid

   #231543

   Purpose

   Expresses the HMG of Salro Sox-like, for protein purification

   Depositor

   Ralf Jauch

   Article

   Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

   Insert

   Sox-Iike HMG
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pETG20a-hCic-HMG

   Plasmid

   #231545

   Purpose

   Expresses the HMG of human Cic, for protein purification

   Depositor

   Ralf Jauch

   Article

   Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

   Insert

   Cic HMG
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pETG20a-Pchi-HMG

   Plasmid

   #231538

   Purpose

   Expresses the HMG of Pchi Sox, for protein purification

   Depositor

   Ralf Jauch

   Article

   Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

   Insert

   Sox HMG
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETG20a-Monbr-HMG

   Plasmid

   #231544

   Purpose

   Expresses the HMG of Monbr Sox-like, for protein purification

   Depositor

   Ralf Jauch

   Article

   Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

   Insert

   Sox-Iike HMG
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pETG20a-Salhel-HMG

   Plasmid

   #231537

   Purpose

   Expresses the HMG of Salhel Sox-I, for protein purification

   Depositor

   Ralf Jauch

   Article

   Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

   Insert

   Sox-I HMG
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   March 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HemH-R212A_pET-14b

   Plasmid

   #233262

   Purpose

   Overexpress his-tag HemH protein with a site directed mutagenesis, arginine at position 212 was substituted with alanine (R121A)

   Depositor

   Andrew Emili, Mohan Babu

   Article

   Peng et al Cell. 2025 Jan 22:S0092-8674(25)00032-7.

   Insert

   HemH (hemH )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Arginine was replaced with Alanine at position 21…

   Promoter

   T7 promoter

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET-Impact-T4gp41

   Plasmid

   #228207

   Purpose

   Expression in BL21(DE3) of T4 helicase

   Depositor

   Stephen Benkovic

   Insert

   41 (41 Escherichia phage T4)
   

   Use

   Tags

   Sce VMA intein-chitin binding domain

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   March 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET-Impact-T4gp59

    Plasmid

    #228206

    Purpose

    Expression in BL21(DE3) of WT T4 helicase loader

    Depositor

    Stephen Benkovic

    Insert

    59 (59 Escherichia phage T4)
    

    Use

    Tags

    Sce VMA intein-chitin binding domain

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET28-NFHΔ462-638

    Plasmid

    #232061

    Purpose

    Expresses construct for surface tethering: R. norvegicus NFH tail domain lacking residues 462-638

    Depositor

    Sanjay Kumar

    Article

    Ding et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

    Insert

    Neurofilament-Heavy tail domain lacking residues 462-638
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    added N-terminal tryptophan and tyrosine, and cys…

    Promoter

    Available Since

    March 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET14-H2Kb-ST3

    Plasmid

    #223221

    Purpose

    Expression of mouse MHC H2Kb heavy chain fused to Spytag003 under T7 promoter for expression in bacteria

    Depositor

    Omer Dushek

    Article

    Patel et al EMBO J. 2024 Jan;43(1):132-150. doi: 10.

    Insert

    Expression of soluble mouse MHC H2KB heavy chain (HLA-A Mouse)
    

    Use

    Tags

    Spytag

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pU6-BoxB-petracrRNA

    Plasmid

    #207625

    Purpose

    Expresses BoxB-petracrRNA in mammalian cells (with a human-U6 promoter)

    Depositor

    Jay Shendure

    Article

    Choi et al Elife. 2025 Feb 12;13:RP98110. doi: 10.7

    Insert

    BoxB-petracrRNA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available Since

    Feb. 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET28_Strep-Psc-pScaC

    Plasmid

    #228834

    Purpose

    E. coli expression of the ScaC passenger domain

    Depositor

    Andrew Carter

    Article

    Manigrasso et al bioRxiv 2024.04.11.589026

    Insert

    ScaC
    

    Use

    Tags

    Strep

    Expression

    Bacterial

    Mutation

    aa 33-223

    Promoter

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-OuIAD(E502Q)

    Plasmid

    #226973

    Purpose

    Expressing Olsenella uli indoleacetate decarboxylase (IAD) E502Q variant in E. coli

    Depositor

    Emily Balskus

    Article

    Fu et al J Am Chem Soc. 2022 Jun 29;144(25):11110

    Insert

    OuIAD(E502Q)
    

    Use

    Tags

    His6 (on backbone)

    Expression

    Bacterial

    Mutation

    E502Q

    Promoter

    Available Since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET21-pelB-GST-7

    Plasmid

    #228989

    Purpose

    For bacterial expression of anti-GST nanobody GST-7, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    GST-7
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21-pelB-LaG94-9

    Plasmid

    #228968

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-9, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-9
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET21-pelB-LaG94-10

    Plasmid

    #228969

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-10, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-10
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21-pelB-LaG94-12

    Plasmid

    #228970

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-12, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-12
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET21-pelB-LaG94-3

    Plasmid

    #228963

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-3, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-3
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET21-pelB-LaTdT-38

    Plasmid

    #228979

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-38, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-38
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET21-pelB-LaG94-8

    Plasmid

    #228967

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-8, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-8
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET21-pelB-LaG94-6

    Plasmid

    #228966

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-6, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-6
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET21-pelB-LaG94-18

    Plasmid

    #228972

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-18, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-18
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET21-pelB-LaTdT-2

    Plasmid

    #228975

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-2
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pET21-pelB-LaGIgG-4

    Plasmid

    #228998

    Purpose

    For bacterial expression of anti-mouse IgG nanobody LaMIgG-4, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaGIgG-4
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pET21-pelB-LaG94-2

    Plasmid

    #228962

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-2, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-2
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET21-pelB-LaG94-1

    Plasmid

    #228961

    Purpose

    For bacterial expression of anti-GFP nanobody LaG94-1, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaG94-1
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET21-pelB-LaMIgG-9

    Plasmid

    #228993

    Purpose

    For bacterial expression of anti-mouse IgG nanobody LaMIgG-9, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaMIgG-9
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pET21-pelB-LaTdT-44

    Plasmid

    #228981

    Purpose

    For bacterial expression of anti-tdTomato nanobody LaTdT-44, with pelB leader for periplasmic secretion, and C-term 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

    Insert

    LaTdT-44
    

    Use

    Tags

    6xHIS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge