We narrowed to 12,851 results for: LIC

1. pAAV-SEPT_Cdk2-T160A-siR

   Plasmid

   #73975

   Purpose

   HDR template to generate Cdk2-T160A-siR

   Depositor

   Steven Dowdy, Manuel Kaulich

   Article

   Kaulich et al Nucleic Acids Res. 2015 Apr 20;43(7):e45

   Inserts

   Cdk2-LHA-siR (CDK2 Human)
   Cdk2-RHA-T160A (CDK2 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   T160 mutated to Ala and siRNA target site mutated

   Promoter

   Available since

   March 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCW57-mCherry-2A-BRD4 Iso AdelCTD

   Plasmid

   #137722

   Purpose

   Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long isoform missing its C-terminal, P-TEFb interacting domain (CTD)

   Depositor

   Scott Floyd

   Article

   Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

   Insert

   BRD4 long isoform without its C-terminal, P-TEFb interacting domain (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Truncated at amino acid 1328

   Promoter

   Tight TRE promoter

   Available since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW57-mCherry-2A-BRD4 Iso CdelET

   Plasmid

   #137723

   Purpose

   Doxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)

   Depositor

   Scott Floyd

   Article

   Edwards et al Cell Rep. 2020 Sep 22;32(12):108166. doi

   Insert

   BRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deleted amino acids 600-682

   Promoter

   Tight TRE promoter

   Available since

   Jan. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLPC-NMYC-hTRF1deltaBLM

   Plasmid

   #64165

   Purpose

   Retroviral vector expressing human TRF1 delta aa317-374 with N-terminal MYC tag

   Depositor

   Titia de Lange

   Article

   Zimmermann et al Genes Dev. 2014 Nov 15;28(22):2477-91. d

   Insert

   hTRF1 (TERF1 Human)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   deleted amino acids aa317-374 of human TRF1

   Promoter

   CMV

   Available since

   May 29, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pAAV-SEPT_Cdk2-T160E-siR

   Plasmid

   #73976

   Purpose

   HDR template to generate Cdk2-T160E-siR

   Depositor

   Steven Dowdy, Manuel Kaulich

   Article

   Kaulich et al Nucleic Acids Res. 2015 Apr 20;43(7):e45

   Inserts

   Cdk2-LHA-siR (CDK2 Human)
   Cdk2-RHA-T160E (CDK2 Human)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   T160 mutated to Glu and siRNA target site mutated

   Promoter

   Available since

   March 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLX209-neo-WRN K577M

   Plasmid

   #125790

   Purpose

   constitutive, ectopic expression of a helicase-dead mutant of human WRN (K577M)

   Depositor

   Francisca Vazquez

   Article

   Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

   Insert

   myc-WRN K577M (WRN Human)
   

   Use

   Tags

   5x myc

   Expression

   Mammalian

   Mutation

   K577M (helicase-dead)

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLX209-neo-WRN E84A K577M

   Plasmid

   #125791

   Purpose

   constitutive, ectopic expression of an endonuclease/helicase-dead mutant of human WRN

   Depositor

   Francisca Vazquez

   Article

   Chan et al Nature. 2019 Apr 10. pii: 10.1038/s41586

   Insert

   myc-WRN E84A/K577M (WRN Human)
   

   Use

   Tags

   5x myc

   Expression

   Mammalian

   Mutation

   E84A/K577M (exonuclease/helicase-dead)

   Promoter

   Available since

   June 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pWZL Neo Myr Flag CAMK1G

   Plasmid

   #20438

   Depositor

   Jean Zhao, William Hahn

   Article

   Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

   Insert

   CAMK1G (CAMK1G Human)
   

   Use

   Retroviral

   Tags

   Flag and Myr

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2009

   Availability

   Academic Institutions and Nonprofits only

9. pLV-hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110)-IRES-Blast

   Plasmid

   #193139

   Purpose

   Expresses bicistronic P2A construct of C-CRL4Cdt2 reporter(hCdt1(1-100)ΔCy-mCherry-) and APC/C reporter (hGeminin(1-110)) with blasticidin resistance

   Depositor

   Tobias Meyer

   Article

   Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

   Insert

   hCdt1(1-100)ΔCy-mCherry-P2A-mVenus-hGeminin(1-110) (CDT1, GMNN Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   human Cdt1 amino acids 1-100 w/ ΔCy mutation (aa6…

   Promoter

   EF1a

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. SRC_HUMAN_D0

    Plasmid

    #79700

    Purpose

    This plasmid encodes the kinase domain of SRC. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    SRC (SRC Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1 6B codon-optimized NLRP1 CARD-mCherry WT

    Plasmid

    #163991

    Purpose

    Mammalian expression vector encoding codon-optimized human NLRP1 CARD with a C-terminal mCherry tag for imaging

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. ABL1_HUMAN_D0

    Plasmid

    #79727

    Purpose

    This plasmid encodes the kinase domain of ABL1. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    ABL1 (ABL1 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA3.1 6A NLRP1 CARD-FLAG WT

    Plasmid

    #163978

    Purpose

    Mammalian expression vector encoding human NLRP1 CARD with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 CARD (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. OA-1136J

    Plasmid

    #153023

    Purpose

    Express His-MBP-TEV-CasRx recombinant protein

    Depositor

    Omar Akbari

    Article

    Brogan et al ACS Sens. 2021 Nov 26;6(11):3957-3966. d

    Insert

    CasRx
    

    Use

    Tags

    His-MBP-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DYR1A_HUMAN_D0

    Plasmid

    #79690

    Purpose

    This plasmid encodes the kinase domain of DYR1A. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    DYRK1A (DYRK1A Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pET His6 MBP TEV HDAC8_374

    Plasmid

    #122174

    Purpose

    Expresses a truncated construct of histone deacetylase 8 for crystallography bearing an N-terminal tobacco etch virus protease-cleavable hexahistidine-maltose binding protein tag

    Depositor

    David Christianson

    Article

    Porter et al ACS Chem Biol. 2017 Sep 15;12(9):2281-22

    Insert

    His6 MBP TEV HDAC8_374 (HDAC8 Human)
    

    Use

    Tags

    hexahistidine tag and tobacco etch virus protease…

    Expression

    Bacterial

    Mutation

    Residues Met1–Pro7 and H375-V377 have been delete…

    Promoter

    T7

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AURKA_HUMAN_D0

    Plasmid

    #79739

    Purpose

    This plasmid encodes the kinase domain of AURKA. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    AURKA (AURKA Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

18. pcDNA3.1 6A NLRP1 UPA-CARD-FLAG M1457A

    Plasmid

    #163988

    Purpose

    Mammalian expression vector encoding human NLRP1 UPA-CARD (dimer interface mutant) with a C-terminal FLAG tag

    Depositor

    Hao Wu

    Article

    Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

    Insert

    NLRP1 UPA-CARD (NLRP1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    M1457A

    Promoter

    Available since

    May 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. NEK1_HUMAN_D0

    Plasmid

    #79725

    Purpose

    This plasmid encodes the kinase domain of NEK1. Intended for co-expression with Lambda Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    NEK1 (NEK1 Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

20. MERTK_HUMAN_D0

    Plasmid

    #79705

    Purpose

    This plasmid encodes the kinase domain of MERTK. Intended for co-expression with YopH Phosphatase that accompanies this set to enhance bacterial kinase expression.

    Depositor

    John Chodera, Nicholas Levinson, Markus Seeliger

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    MERTK (MERTK Human)
    

    Use

    Tags

    His10-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only