We narrowed to 17,819 results for: Mut

1. Progerin-S22D-CS-pLPC

   Plasmid

   #73810

   Purpose

   Progerin mutant where Serine 22 is mutated to aspartic acid and where there is a substitution of cysteine in CaaX-motif by a serine

   Depositor

   Gerardo Ferbeyre

   Article

   Moiseeva et al Aging (Albany NY). 2016 Feb;8(2):366-81.

   Insert

   Progerin (LMNA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Progerin with substitution of cysteine in CaaX-mo…

   Promoter

   CMV

   Available Since

   April 1, 2016

   Availability

   Academic Institutions and Nonprofits only

2. pLPC-S22AProgerin-CS

   Plasmid

   #69063

   Purpose

   encodes a Progerin mutant with serine 22 mutated to an alanine and with a substitution of cysteine in CaaX-motif by a serine

   Depositor

   Gerardo Ferbeyre

   Article

   Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

   Insert

   Progerin (LMNA Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Progerin with serine 22 mutated to alanine and wi…

   Promoter

   CMV

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

3. CA-RIT-NFAT1 (IRES-GFP)

   Plasmid

   #85181

   Purpose

   Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interaction. Co-expresses EGFP for selection

   Depositor

   Anjana Rao

   Article

   Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15

   Insert

   CA-RIT-NFAT1 (Nfatc2 Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Amino acids 1-3 removed; CA: PASSGSSASF mutated t…

   Promoter

   Available Since

   April 12, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pDEST53-LRRK2-3XKD

   Plasmid

   #25049

   Depositor

   Mark Cookson

   Article

   Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

   Insert

   LRRK2 (LRRK2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   "Kinase Dead" Lysine 1906 mutated to A…

   Promoter

   Available Since

   June 17, 2010

   Availability

   Academic Institutions and Nonprofits only

5. G protein alpha-s(alpha3beta5/G226A)

   Plasmid

   #55798

   Purpose

   In addition to the mutations in alpha-s(alpha3beta5), this dominant negative G protein alpha-s mutant contains the G226A mutation, which impairs activating conformation changes in Switch II.

   Depositor

   Catherine Berlot

   Article

   Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

   Insert

   alpha-s (alpha3beta5/G226A) (Gnas Rat)
   

   Use

   Tags

   internal EE epitope (residues 189-194 in alpha-s …

   Expression

   Mammalian

   Mutation

   N271K, K274D, R280K, T284D, I285T, G226A in alpha…

   Promoter

   CMV

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. CA-RIT-NFAT1 (PGK-Puro)

   Plasmid

   #63214

   Purpose

   Constitutively active NFAT1, mutated to interfere selectively with the NFAT:AP-1 interactio. Contains Puromycin selection marker

   Depositor

   Anjana Rao

   Article

   Martinez et al Immunity. 2015 Feb 6. pii: S1074-7613(15

   Insert

   CA-RIT-NFAT1 (Nfatc2 Mouse)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Amino acids 1-3 removed; CA: PASSGSSASF mutated t…

   Promoter

   Available Since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBABE-hygro-BAP1-G312G-HA

   Plasmid

   #154022

   Purpose

   Mammalian Expression of HA-tagged BAP1 with the Synonymous Mutation p.G312G

   Depositor

   Samuel Peña-Llopis

   Article

   Niersch et al iScience. 2021 Feb 12;24(3):102173. doi:

   Insert

   BAP1 (BAP1 Human)
   

   Use

   Retroviral

   Tags

   Hemagglutinin (HA)

   Expression

   Mammalian

   Mutation

   c.936T>G, p.G312G (Synonymous Mutation) by Sit…

   Promoter

   Available Since

   Feb. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLX313-TP53-P278A

   Plasmid

   #118015

   Purpose

   Constitutive expression of mutant human Tumor Protein p53 (TP53)

   Depositor

   William Hahn, David Root

   Article

   Giacomelli et al Nat Genet. 2018 Oct;50(10):1381-1387. do

   Insert

   TP53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   p.Pro278Ala

   Promoter

   EF1alpha

   Available Since

   Oct. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. G protein alpha-s(alpha3beta5/G226A/A366S)

   Plasmid

   #55797

   Purpose

   This highly effective dominant negative G protein alpha-s mutant contains, in addition to the mutations in alpha-s(alpha3beta5/G226A), the A366S mutation, which increases GDP release.

   Depositor

   Catherine Berlot

   Article

   Berlot et al J Biol Chem. 2002 Jun 7;277(23):21080-5.

   Insert

   alpha-s (alpha3beta5/G226A/A366S) (Gnas Rat)
   

   Use

   Tags

   internal EE epitope (residues 189-194 in alpha-s …

   Expression

   Mammalian

   Mutation

   N271K, K274D, R280K, T284D, I285T, G226A, A366S i…

   Promoter

   CMV

   Available Since

   Aug. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Flag_HsIRE1a_deltaP29_D408_K599A_pBabePuro

    Plasmid

    #58492

    Purpose

    retroviral pBabe puro vector encoding N-terminally FLAG tagged mutant human IRE1a deleted from amino acids P28 to D408 and bearing mutation K599A (kinase domain mutant)

    Depositor

    David Ron

    Article

    Volmer et al Proc Natl Acad Sci U S A. 2013 Mar 19;11

    Insert

    IRE1a (ERN1 Human)
    

    Use

    Retroviral

    Tags

    FLAG and preprotrypsin signal sequence

    Expression

    Mammalian

    Mutation

    deleted amino acids P29 to D408 and bearing mutat…

    Promoter

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKan cpRBP-FL P53

    Plasmid

    #174504

    Purpose

    Express Full-length P53 in E.coli with cleavable expression/purification tag (ribose binding protein circular permutant)

    Depositor

    Stewart Loh

    Article

    Ha et al Bio Protoc. 2021 Oct 20;11(20):e4188. do

    Insert

    P53 gene (TP53 Human)
    

    Use

    Tags

    20 amino acid linker containing HRV3C protease si…

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Oct. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHSS6

    Plasmid

    #37060

    Depositor

    Hank Seifert, Fred Heffron

    Article

    Seifert et al Proc Natl Acad Sci U S A. 1986 Feb;83(3)

    Type

    Empty backbone

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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13. pxCas9CR4

    Plasmid

    #111656

    Purpose

    PCas9CR4 with the xCas9 mutations allowing NG PAM sites

    Depositor

    Christopher Reisch

    Insert

    TetR and xCas9
    

    Use

    Synthetic Biology

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    xCas9 mutations

    Promoter

    Available Since

    July 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. PRS303-240xtetO

    Plasmid

    #44754

    Depositor

    Narendra Maheshri

    Article

    Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

    Insert

    240x tetO array and CHRI homology
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Oct. 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBS-85xtetO-KlURA3

    Plasmid

    #44757

    Depositor

    Narendra Maheshri

    Article

    Finney-Manchester et al Nucleic Acids Res. 2013 Mar 7.

    Insert

    85x tetO array + KlURA3 marker + CHRI1 homology
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pJK626

    Plasmid

    #72461

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Cys/Tyr154>Phe mutant (AaPurE1-S57C,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutation changes serine-57 to cysteine, tyrosine-…

    Promoter

    T7

    Available Since

    March 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pJK627

    Plasmid

    #72462

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Asp/Tyr154>Phe mutant (AaPurE1-S57D,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutation changes serine-57 to aspartate, tyrosine…

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJK628

    Plasmid

    #72463

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Ala/Tyr154>Phe mutant (AaPurE1-S57A,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutation changes serine-57 to alanine, tyrosine-1…

    Promoter

    T7

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pJK632

    Plasmid

    #72465

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Val/Tyr154>Phe mutant (AaPurE1-S57V,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutation changes serine-57 to valine, tyrosine-15…

    Promoter

    T7

    Available Since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pJK629

    Plasmid

    #72464

    Purpose

    Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide mutase, Ser57>Thr/Tyr154>Phe mutant (AaPurE1-S57T,Y154F)

    Depositor

    T. J. Kappock

    Insert

    N5-carboxyaminoimidazole ribonucleotide mutase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Mutation changes serine-57 to threonine, tyrosine…

    Promoter

    T7

    Available Since

    Feb. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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