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We narrowed to 9,134 results for: Pol

Showing: 1601 - 1620 of 9134 results

  6. pET21b(+)-Is-PETase-W159H-S238F

    Plasmid
    #112203
    Purpose
    pET-21b(+) based plasmid for expression of PETase from Ideonella sakaiensis 201-F6 (Genbank GAP38373.1) with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Depositor
    Christopher Johnson, Gregg Beckham
    Article
    Austin et al Proc Natl Acad Sci U S A. 2018 May 8;115
    Insert
    PETase gene from Ideonella sakaiensis 201-F6 with W159H and S238F mutations, codon optimized for expression in E. coli K12
    Tags
    6XHIS
    Expression
    Bacterial
    Mutation
    W159H, S238F
    Promoter
    N/A
    Available Since
    July 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    112203-plasmid-map-sequence-id-473446

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  8. pEN487 - Sororin-AID[71-114]-eGFP-FRT-Blast-FRT targeting conuct

    Plasmid
    #156433
    Purpose
    Targeting vector to introduce an AID-eGFP cassette at the mouse Cdca5 (SORORIN) locus using BLASTICIDIN selection. Auxin-inducible degron system. Designed to be used with sgRNA GGGATGCCCGTCATTAAGTG
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    SORORIN, AID (Cdca5 Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    none
    Available Since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    156433-plasmid-map-sequence-id-310157

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  9. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-P90C

    Plasmid
    #114606
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P90C cysteine mutations and a 42 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, P…
    Available Since
    Oct. 25, 2018
    Availability
    Academic Institutions and Nonprofits only
    114606-plasmid-map-sequence-id-225647

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  10. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-P80C

    Plasmid
    #114602
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P80C cysteine mutations and a 22 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, P…
    Available Since
    Oct. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114602-plasmid-map-sequence-id-224743

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  11. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-A60C

    Plasmid
    #114601
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & A60C cysteine mutations and a 22 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, A…
    Available Since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114601-plasmid-map-sequence-id-222795

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  12. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-P70C

    Plasmid
    #114605
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P70C cysteine mutations and a 42 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, P…
    Available Since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114605-plasmid-map-sequence-id-222758

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  13. pTWIN1-His6-Ssp-Htt18-90(Q18C)-42Q-A60C

    Plasmid
    #114604
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & A60C cysteine mutations and a 42 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, A…
    Available Since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114604-plasmid-map-sequence-id-222736

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  14. pTWIN1-His6-Ssp-Htt18-90(Q18C)-22Q-P90C

    Plasmid
    #114603
    Purpose
    Bacterial expression of Htt fragment containing amino acids 18-90, with Q18C & P90C cysteine mutations and a 22 polyQ repeat
    Depositor
    Hilal Lashuel
    Article
    Warner JB et al J Am Chem Soc. 2017 Oct 18;139(41):14456
    Insert
    Huntingtin Exon1 fragment (HTT Human)
    Tags
    His6-Ssp DnaB intein
    Expression
    Bacterial
    Mutation
    dNt17 (deletion of first 17 amino acids), Q18C, P…
    Available Since
    Sept. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    114603-plasmid-map-sequence-id-222733

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  15. pAU002 - pTRE3G-CTCF(DELTA1-265)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156429
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    DELTA1-265
    Available Since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156429-plasmid-map-sequence-id-310209

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  16. pEN515 - pTRE3G-CTCF(ZFmut)-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156434
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA (all Zinc fingers point mutated), targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    All zinc fingers mutated
    Available Since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156434-plasmid-map-sequence-id-310270

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  17. pAU003 - pTRE3G-Cterm_Nterm_switch_CTCF-mRuby2-CAGGS-rtta3G-Frt-PGK-PuroR-bpA-Frt TIGRE

    Plasmid
    #156430
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible mutant CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    Cterm_Nterm_switched
    Available Since
    Sept. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    156430-plasmid-map-sequence-id-310203

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  19. pEN366 - pTRE3G-CTCF-mRuby2-BGHpA-CAGGS-rtta3G-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt TIGRE donor

    Plasmid
    #156432
    Purpose
    Vector to introduce a constitutive rtta3G cassette and a DOX-inducible wild-type CTCF mouse cDNA, targeting the mouse TIGRE acceptor locus. Use with Addgene #92144 sgRNA. Use puromycine selection.
    Depositor
    Elphege Nora
    Article
    Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:
    Insert
    CTCF, rtta3G, TetO3G (Ctcf Mouse)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Mutation
    none
    Available Since
    Sept. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    156432-plasmid-map-sequence-id-310156

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Showing: 1601 - 1620 of 9134 results