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ptdEos-CLC

Plasmid

#38009

Depositor

Xiaowei Zhuang

Article

Jones et al Nat Methods. 2011 Jun;8(6):499-508. Epub

Inserts

Clathrin, light polypeptide (LCa) (Clta Mouse)
Eos FP, tandem dimer

Expression

Bacterial and Mammalian

Promoter

CMV

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) EPHB4 Antisense

Plasmid

#60640

Purpose

Negative Control for the pcDNA3.1(+) EPHB4 Sense plasmid

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

Ephrin type B receptor 4 (EPHB4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pEGFP-hSyntenin2b

Plasmid

#89437

Purpose

mammalian expression of EGFP tagged human Syntenin2b

Depositor

Fritz Rathjen

Article

Koroll et al J Biol Chem. 2001 Apr 6;276(14):10646-54

Insert

SDCBP2 (SDCBP2 Human)

Tags

EGFP

Expression

Mammalian

Mutation

*see below

Promoter

CMV

Available Since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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pAcUW21-CqJHE

Plasmid

#84213

Purpose

baculovirus transfer vector containing cqjhe coding sequence from Culex quinquefasiatus

Depositor

Bruce Hammock

Article

Kamita et al PLoS One. 2011;6(12):e28392. doi: 10.137

Insert

JHE

Expression

Insect

Promoter

p10

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only
pFBOH-MHL_EIF3B:127-814

Plasmid

#210909

Purpose

Insect expression of human EIF3B fragment D127 to E814. N-terminal 6X His tag with TEV cleavage.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

EIF3B:D127-E814 (EIF3B Human)

Tags

MHHHHHHSSGRENLYFQGMQ

Expression

Insect

Mutation

Y760C sequence conflict

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCEP4-myc-EphB4

Plasmid

#200983

Purpose

Mammalian expression plasmid for myc-tagged EphB4

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB4 (EPHB4 Human)

Tags

Extracellular c-myc epitope tag and Signal peptid…

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EphB4-Fc

Plasmid

#200986

Purpose

Mammalian expression plasmid for soluble EphB4 fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EphB4 (EPHB4 Human)

Tags

Fc region of human IgG1

Expression

Mammalian

Mutation

Soluble ectodomain of EphB4

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pBABR IRFP-Bcd-LEXY

Plasmid

#182595

Purpose

Drosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.

Depositor

Jared Toettcher

Article

Singh et al Cell Rep. 2022 Mar 22;38(12):110543. doi

Insert

Bcd (bcd Fly)

Tags

IRFP and LEXY

Expression

Insect

Promoter

maternal tubulin

Available Since

Aug. 22, 2022

Availability

Academic Institutions and Nonprofits only

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EPHB4 gRNA (BRDN0001145313)

Plasmid

#76354

Purpose

3rd generation lentiviral gRNA plasmid targeting human EPHB4

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

EPHB4 (EPHB4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-3A2-mCherry

Plasmid

#73425

Purpose

Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3A2.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

Promoter 3A2 (orthogonal T7-lac variant)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

P3A2 (orthogonal T7-lac variant)

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-3F2-mCherry

Plasmid

#73418

Purpose

Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 3F2.

Depositor

Mattheos Koffas

Article

Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

Insert

Promoter 3F2 (orthogonal T7-lac variant)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Promoter

P3F2 (orthogonal T7-lac variant)

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pFB-Flag-MyoD-MyoD

Plasmid

#25992

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD-MyoD homodimer (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only
pFHMSPN-avi-TEV-LIC V

Plasmid

#220885

Purpose

Donor vector to generate recombinant baculovirus by site-specific transposition in an E. coli host.

Depositor

Cheryl Arrowsmith

Type

Empty backbone

Use

Baculovirus expresssion

Tags

Honeybee melittin signal followed by His, avi and…

Available Since

June 20, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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p3xFLAG-CMV TIP60 390-513

Plasmid

#78797

Purpose

To overexpress TIP60 390-513 in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al Cell Death Differ. 2013 Apr;20(4):620-9.

Insert

TIP60 (KAT5 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

aa390-513

Promoter

CMV

Available Since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pFBOH-LIC_DDB1:1-1140_delta:396-689

Plasmid

#210897

Purpose

Insect expression of full-length human DDB1 with a loop deletion I396 to D689. Untagged.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

DDB1:M1-H1140_delta:I396-D689 (DDB1 Human)

Expression

Insect

Mutation

loop deletion amino acids I396 to D689

Promoter

Polyhedrin

Available Since

March 19, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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MIS18A_pLENTI-CAG-IRES-GFP

Plasmid

#176996

Purpose

Mammalian lentiviral expression vector encoding MIS18A

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

MIS18A (MIS18A Human)

Use

Lentiviral

Promoter

CAG

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDGE1176

Plasmid

#202774

Purpose

CRISPR-KO in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:zCas9_tNbEU
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pVMG0193_Cq-Rpl40-Cas9

Plasmid

#169346

Purpose

Expression of Cas9 in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0193_Cq-Rpl40-Cas9

Use

CRISPR

Expression

Insect

Promoter

CPIJ002413

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pVMG0217_Cq-U6-2b_2xBbsI-gRNA

Plasmid

#169339

Purpose

Expression of gRNA in Culex quinquefasciatus or other mosquitoes

Depositor

Valentino Gantz

Article

Feng et al Nat Commun. 2021 May 20;12(1):2960. doi:

Insert

pVMG0217_Cq-U6-2b_2xBbsI-gRNA

Use

CRISPR

Expression

Insect

Promoter

CPIJ039728

Available Since

May 21, 2021

Availability

Academic Institutions and Nonprofits only

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pDGE1177

Plasmid

#202775

Purpose

CRISPR-KO with TRE2 exonuclease co-expression in Arabidopsis thaliana

Depositor

Johannes Stuttmann

Article

Ordon et al Plant Methods. 2023 Mar 28;19(1):30. doi

Inserts

FAST marker
pRPS5a:TREX2-zCas9_tNbEU
ccdB cassette (BsaI-excisable)

Use

CRISPR

Expression

Plant

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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