We narrowed to 2,522 results for: plenti

1. pLenti CMV TRE3G Puro FLAG-DD-ER-mCherry-TRF1-FokI D450A

   Plasmid

   #207953

   Purpose

   nuclease inactive control for TRF1FokI WT

   Depositor

   Roger Greenberg

   Article

   Dilley et al Nature. 2016 Nov 3;539(7627):54-58. doi:

   Insert

   TRF1 (TERF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag, DD, ER, mCherry and FokI (D450A)

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Oct. 19, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-CMV-Myr-Pink Flamindo-p2a-EGFP-bPAC-CAAX

   Plasmid

   #202719

   Purpose

   Mammalian co-expression of membrane-bound Pink Flamindo and membrane-bound bPAC for cAMP perturbation and imaging

   Depositor

   Adam Cohen

   Article

   Xiang et al bioRxiv 2023

   Inserts

   Myr-Pink Flamindo
   EGFP-bPAC-CAAX
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-mCherry

   Plasmid

   #122959

   Purpose

   Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with mCherry expression

   Depositor

   Pierre Vanderhaeghen

   Article

   Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

   Insert

   NOTCH2NL-delta C terminus (NOTCH2NLB Human)
   

   Use

   Lentiviral

   Tags

   6xmyc tag

   Mutation

   deletion of C terminus from full length NOTCH2NLB

   Promoter

   CAG promoter

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-CAG-NOTCH2NL-delta C terminus (N2NL-delta C)-ires-EGFP

   Plasmid

   #122956

   Purpose

   Lentiviral expression of NOTCH2NL delta C terminus under CAG promoter with EGFP expression

   Depositor

   Pierre Vanderhaeghen

   Article

   Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

   Insert

   NOTCH2NL-delta C terminus (NOTCH2NLB Human)
   

   Use

   Lentiviral

   Tags

   6xhis tag

   Mutation

   deletion of C terminus from full length NOTCH2NLB

   Promoter

   CAG promoter

   Available Since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti EFS-GFP-pa (opposite orientation)- TRE3g-Flag-GluN2B-WPRE

   Plasmid

   #233047

   Purpose

   To express GFP from an EFS promoter in the 3'-5' direction and Flag-GluN2B from a TRE3g promoter in the 5'-3' direction

   Depositor

   Jonathan Ploski

   Insert

   GFP and GluN2B
   

   Use

   Lentiviral

   Tags

   Flag and GFP

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLentiRNAGuide_004-hU6-RfxCas13d-DR1-BsmBI-EFS-EGFP-P2A-Puro-WPRE

   Plasmid

   #223175

   Purpose

   For cloning of guide RNAs libraries compatible with RfxCas13d. Contains a 5' modified direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa

   Depositor

   Neville Sanjana

   Article

   Hart et al bioRxiv [Preprint]. 2024 May 14:2024.05.

   Insert

   hU6_Rfxcas13d-DR1_BsmBI
   

   Use

   Lentiviral

   Mutation

   DR1 A>T

   Available Since

   Jan. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-Ef1a-mClover-W377A/W428A YTHDC1-T2A-BSD-siRNA-Resistant

   Plasmid

   #177130

   Purpose

   Generate lentivirus to introduce mClover tagged m6A binding null mutant W377A/W428A YTHDC1

   Depositor

   Wenbo Li

   Article

   Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e

   Insert

   YTHDC1 (YTHDC1 Human)
   

   Use

   Lentiviral

   Tags

   mClover3

   Mutation

   W428A/W377A (m6A binding defects), and siRNA resi…

   Promoter

   Ef1a

   Available Since

   July 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

   Plasmid

   #163643

   Purpose

   Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.

   Depositor

   Chang Liu

   Article

   Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d

   Inserts

   pU6-CHYRON17 hgRNA
   Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
   

   Use

   Lentiviral

   Tags

   Luc2-P2A-tdTomato-T2A

   Mutation

   The SpCas9 sgRNA constant region is mutated to ma…

   Promoter

   EFS and pU6

   Available Since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiRNACRISPR_010-TetO-NLS-RfxCas13d-2A-mCherry-NLS-WPRE-EFS-rtTA3-2A-Blast

   Plasmid

   #228555

   Purpose

   Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

   Depositor

   Neville Sanjana

   Article

   Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

   Insert

   RfxCas13d
   

   Use

   Lentiviral

   Tags

   NLS-HA and P2A-mCherry

   Available Since

   Aug. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiRNACRISPR_011-TetO-NLS-inactive-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #228557

    Purpose

    Expresses inactive RfxCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

    Insert

    dRfxCas13d
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Mutation

    R239A/H244A/R858A/H863A

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiRNACRISPR_012-TetO-NLS-inactive-hfCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

    Plasmid

    #228558

    Purpose

    Expresses inactive hfCas13d in mammalian cells for binding of target RNAs in combination with compatible crRNAs. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Hart et al Nat Biotechnol. 2025 Feb 11. doi: 10.103

    Insert

    dhfCas13d (N2V8)
    

    Use

    Lentiviral

    Tags

    NLS-HA

    Mutation

    R239A/H244A/R858A/H863A

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiX1-[TRE3G-EGFP-FMRP-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

    Plasmid

    #235306

    Purpose

    Lentiviral expression of ComMAND base gene regulating EGFP-FMRP (therapeutically relevant gene)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    EGFP-Fmr1 (Fmr1 Mouse)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti_CHyMErA.v2_U6(SpCas9_I-SceI)-(AsCas12a(dual-gRNA)_PacI-PacI)_PGK-puro

    Plasmid

    #189636

    Purpose

    Lentiviral expression of single SpCas9 and dual AsCas12a gRNAs for generating combinatorial CHyMErA.v2 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    hU6 Cas9-Cas12a-Cas12a gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_mpx_AsCas12a(dual-gRNA)_h7SK(PacI-ClaI)_U6(I-SceI-NheI)_PGK-puro

    Plasmid

    #189635

    Purpose

    Lentiviral expression of double dual-AsCas12a gRNAs for generating combinatorial AsCas12a 3Cs libraries

    Depositor

    Manuel Kaulich

    Article

    Cetin et al Sci Rep. 2023 May 6;13(1):7405. doi: 10.

    Insert

    h7SK arrayed Cas12a gRNA cassette, hU6 arrayed Cas12a gRNA cassette, PGK puro cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-CAG-NOTCH2NL-delta EGF repeats (N2NL-delta EGF)-ires-EGFP

    Plasmid

    #122955

    Purpose

    Lentiviral expression of NOTCH2NL delta EGF repeats under CAG promoter with EGFP expression

    Depositor

    Pierre Vanderhaeghen

    Article

    Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

    Insert

    NOTCH2NL-delta EGF repeats (NOTCH2NLB Human)
    

    Use

    Lentiviral

    Tags

    6xhis tag

    Mutation

    deletion of EGF repeats from full length NOTCH2NLB

    Promoter

    CAG promoter

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiX1-[TRE3G-FXN-P2A-mRuby2-bGH]-EFS-rtTA-P2A-mGL-WPRE

    Plasmid

    #235303

    Purpose

    Lentiviral expression of ComMAND base gene regulating FXN-P2A-mRuby2 (therapeutically relevant gene)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    FXN-P2A-mRuby2 (FXN Human)
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLentiX1-[TRE3G-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

    Plasmid

    #235268

    Purpose

    Lentiviral expression of ComMAND base gene regulating mRuby2

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti_EF1α-NES-Kinprola_PKA-mEGFP_bGH-PA term_EF1α_FLAG-SV40NLS-Cas9-NLS-T2A-BSD-WPRE-SV40

    Plasmid

    #233371

    Purpose

    EF1α driven co-expression of the PKA activity recorder Kinprola_PKA fused to mEGFP and Cas9 expression through lentivirus transduction

    Depositor

    Kai Johnsson

    Article

    Sun et al Nat Chem Biol. 2025 Jul 10. doi: 10.1038

    Inserts

    NES-Kinprola_PKA-mEGFP
    FLAG-SV40NLS-Cas9-NLS-T2A-BSD
    

    Use

    Lentiviral

    Tags

    FLAG, NES, and mEGFP

    Promoter

    EF1α

    Available Since

    March 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLentiX1-[TRE3G-TS.FF4x1-mRuby2-bGH]-EFS-rtTA-T2A-mGL-P2A-PuroR-WPRE

    Plasmid

    #235316

    Purpose

    Lentiviral expression of mRuby2 with 5' microRNA target site only (closed-loop)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    mRuby2
    

    Use

    Lentiviral and Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiX1-[TRE3G-EGFP(miRE.FF4)-FMRP-TS.FF6x1-bGH]-EFS-rtTA-P2A-mRuby2-WPRE

    Plasmid

    #235307

    Purpose

    Lentiviral expression of ComMAND open-loop circuit regulating EGFP-FMRP (therapeutically relevant gene)

    Depositor

    Kate Galloway

    Article

    Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

    Insert

    EGFP-Fmr1 (Fmr1 Mouse)
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    TRE3G (3rd-generation Tet system)

    Available Since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge